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Stage 10-17 / Expressed / Zygotic / Tracheal system / Specific tracheal branches / Dorsal trunk

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Result Summary

Number of genes	46
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Probe CG12519 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Non-Expressed
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Posterior spiracles Localized Subcellular localization patterns Perinuclear localization Perinuclear around tracheal nuclei

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

-via HCR-FISH (3 probe sets)

-tracheal expression may be non-specific

Probe GH05754 Links: Permalink

Genes: Ance-5

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Cytoplasmic foci Apical localization Apical enrichment Discrete apical foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Apical localization Apical enrichment Discrete apical foci Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Dorsal trunk Dorsal trunk foci Posterior spiracles Muscles-embryo Muscles - subregion Hemocytes Hemocytes specific area Ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around hemocytes Foci Small Foci Small Foci in tracheal cells Small Foci in hemocytes Small Foci in ectoderm Bigger Foci

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Partly apical and basal excluded, partly apical foci...

Good, strong probe on gel.

Probe RE18408 Links: Permalink

Genes: Best2

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Cell division apparatus Other cell division pattern Embryonic localization patterns
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Cytoplasmic foci Cell division apparatus Other cell division pattern Anterior localization
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Posterior spiracles Specific posterior spiracle pattern Pharynx Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in Pharynx Small Foci in ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Very low probe on gel.

Very interesting patterns.

Salivary glands, probably non-specific.

Probe RE29777 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 8
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Tracheal system Tracheal 'podia' Specific tracheal branches Dorsal trunk Dorsal trunk foci Salivary glands Proventriculus Posterior spiracles Pharynx Malpighian tubules Head Foregut Esophagus Ectoderm CNS Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small foci in Proventriculus Small Foci in Pharynx Small Foci in other tissues Small Foci in Malpighian tubules Small Foci in foregut Small Foci in ectoderm Small Foci in CNS

Larva

Area	Images	Terms
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Midgut allover Expressed in hindgut Hindgut allover Expressed in gastric caeca Gastric caeca allover Borderline between 'not expressed' and 'low expression'
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs particular pattern Leg discs allover
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes allover Brain lobes - Significant area Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Expressed in salivary glands Salivary glands allover
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Muscles allover - Larva
tracheal system		3rd Instar Larval Tissues Tracheal System Expressed in tracheal tubes
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' Lymph Glands Not expressed in lymph glands

Adult

Area	Images	Terms

Comments

Good probe on gel.

Probe CG1440-RB Links: Permalink

Genes: CG1440

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Ubiquitous Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk cortex localization Yolk cortex enrichment Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Ubiquitous Maternal Localized Yolk plasm localization Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Basal localization Basal enrichment Apical localization Apical enrichment
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in API Expression in amnioserosa All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 8
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Segmented pattern Pharynx Neuroblasts Midgut Head Head epidermis Ectoderm Polarized Ectoderm CNS Ventral nerve cord Clypeo-labral bud Brain Atrium Anal pad Ubiquitous

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

-performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform

-appears ubiquitously throughout development with some mesoectodermal enrichment during the germ band elongation phases (appears as stripes)

Probe LD43683 Links: Permalink

Genes: obst-A

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 6-7		Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Salivary glands Salivary glands lumen Ring gland Esophagus Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around tracheal nuclei Perinuclear around salivary gland nuclei Perinuclear around esophagus nuclei Perinuclear 'rings'

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel, a bit low Low expression.

-seems to be missing a few expression patterns reported on BDGP such as ring gland

-possible expression in garland cells?

Probe GH01149 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Apical exclusion
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Basal localization Basal enrichment
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Dorsal trunk Sensory system Salivary glands Midgut Head Ectoderm CNS Ventral nerve cord Foci - VNC Localized Subcellular localization patterns Nuclei-associated localization Foci Small Foci Small Foci in ectoderm Small Foci in CNS

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel

Data from probe RE20382

Antennal primordium.

Probe LD24702 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Cytoplasmic foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Ubiquitous
stage 8-9		Stage 8-9 Expressed Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Salivary glands Posterior spiracles Specific posterior spiracle pattern Germ cell nuclei Gonads CNS Ventral nerve cord Foci - VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in head Small Foci in CNS Small Foci in Brain

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Nice in situ!

Probe LD24679 Links: Permalink

Genes: CG2767

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Dorsal trunk Proventriculus Posterior spiracles Specific posterior spiracle pattern Pharynx Muscles-embryo Muscles - subregion Hindgut Foregut Esophagus Ectoderm Polarized Ectoderm Blastoderm nuclei Atrium Localized Subcellular localization patterns Cell junction associated Apical enrichment Apical ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe.

Probe RE11181 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Non-Expressed Degraded Degraded completely stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Cell junction associated Basal localization Basal enrichment
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Posterior spiracles Hindgut Midgut hindgut border Head Ectoderm Blastoderm nuclei Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in ectoderm Apical enrichment Apical ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Few hindgut foci Expressed in gastric caeca Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc few cells - dots Expressed in leg discs Leg discs particular pattern Leg discs few cells - dots Expressed in eye disc Eye disc few cells - dots Expressed in antennal disc Antennal disc few cells - dots
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes - Significant area Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Cytoplasmic foci Salivary gland - Small foci Salivary gland - apical Salivary gland - lumen Expressed in salivary glands Salivary gland base
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Expressed in heart
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatocytes -TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Distal tip region-TE Not Expressed in distal tip region -TE Seminal Vesicle Expression level-SV Not detected - SV Ejaculatory Duct Expression level-ED Not detected - ED Accessory Gland Expression level-AG Not detected - AG

Comments

More 'foci'

Good probe on gel. Allover with some 'small foci'...still ubiquitous.

More 'foci'

Probe RE24343 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Apical localization Apical enrichment Discrete apical foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Basal localization Basal enrichment
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Salivary glands Salivary glands lumen Muscles-embryo Muscles - subregion Midgut Hindgut Specific hindgut area or cells Hemocytes Head Ectoderm Brain Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in midgut Small Foci in hemocytes Small Foci in amnioserosa

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus - Few foci Expressed in hindgut Subcellular localization in hindgut Hindgut foci Hindgut Axons Particular hindgut area Hindgut - Midgut border Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Morphogenetic furrow Eye disc basal Expressed in antennal disc Antennal disc few cells - dots Antennal disc - center round cells
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Foci in larval muscles edge Expressed in heart
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover

Adult

Area	Images	Terms

Comments

Often, one dot outside the nuclei (perinuclear, but just one 'foci')

Good probe.

Probe RE10255 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Apical localization Apical enrichment Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed
stage 8-9		Stage 8-9 Expressed Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Polarized tracheal localization Salivary glands Salivary glands lumen Pharynx Hemocytes Hemocytes specific area Head Fat body Fat body- specific region Fat body polarized Ectoderm Polarized Ectoderm Blastoderm nuclei Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around hemocytes Membrane associated Foci Small Foci Small Foci in head Small Foci in ectoderm Apical enrichment Apical trachea Apical ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Proventriculus allover Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules few foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Few hindgut foci Hindgut allover Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Few gastric caeca foci Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc few cells - dots Eye disc allover Expressed in antennal disc Antennal disc few cells - dots Antennal disc allover Borderline between 'not expressed' and 'low expression'
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Cytoplasmic foci Salivary gland - Small foci Expressed in salivary glands Salivary glands allover
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Spermatocyte Tail Cyst Cell - TE Spermatocyte Tail Cyst Cell Cytoplasm - TE Round Tail Cyst Cell - TE Round Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Early cyst cell Cytoplasmic in early cyst cell Cyst stem cell Cytoplasmic in cyst stem cell Seminal Vesicle Subcellularly localized - SV Lumenal-SV Non-particulate - SV Cell type - SV Cytoplasmic-SV Membrane enriched - SV Cytoplasmic Foci - SV Ejaculatory Duct Expression level-ED Expressed Low - ED Subcellularly localized - ED Cell type - ED Cytoplasmic-ED Membrane enriched - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Clusters-AG Expression level-AG Expressed Low - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC Main cells -AG Cytoplasmic-MC

Comments

Good probe on gel.

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions'

Probe LD16982 Links: Permalink

Genes: CG4040

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization RNA islands Pole plasm Pole buds Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus Other cell division pattern Embryonic localization patterns Yolk cortex localization Yolk cortex enrichment
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment Posterior localization Pole cell localization Pole cell enrichment Other subcellular pattern Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Maternal Localized Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns Degraded Degraded partially stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Degraded Degraded completely stage 8 Degradation maternal transcripts
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Salivary glands Proventriculus Muscles-embryo Muscles - subregion Midgut Midgut subset Ectoderm CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around midgut nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in muscle cells Small Foci in midgut Small Foci in ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

RNA exhibits cortical enrichment and posterior pole plasm localization.

RNA exhibits pole cell enrichment and is enriched apically in the posterior blastoderm nuclei.

Salivary glands, my be non-specific.

Probe LP07955 Links: Permalink

Genes: CG4655

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Proventriculus Pharynx Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small foci in Proventriculus Small Foci in Pharynx

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe. Small foci.

Probe GH02976 Links: Permalink

Genes: CG4778

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Ubiquitous
stage 8-9		Stage 8-9 Expressed Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Polarized tracheal localization Salivary glands Posterior spiracles Muscles-embryo Muscles - subregion Midgut Midgut subset Fat body Fat body- specific region Ectoderm CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around tracheal nuclei Membrane associated Foci Small Foci Small Foci in tracheal cells Small Foci in muscle cells Small Foci in midgut Small Foci in fat body Small Foci in ectoderm Small Foci in CNS

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Dorsal trunk.

Good probe

Probe LP10922 Links: Permalink

Genes: CG5618

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Non-Expressed
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Dorsal trunk Posterior spiracles Specific posterior spiracle pattern Blastoderm nuclei Localized

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe. Low expression.

Probe GH10366 Links: Permalink

Genes: CG5687

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Non-Expressed
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Dorsal trunk Dorsal trunk foci Salivary glands Salivary glands - specific region Ectoderm Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in salivary glands Small Foci in ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel.

I see foci inside the tracheal lumen and at other locations as well.

Probe RE19838 Links: Permalink

Genes: CG5756

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Non-Expressed
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Dorsal trunk Salivary glands Salivary glands lumen Head Ectoderm Polarized Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in ectoderm Apical enrichment Apical ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Not expressed in fat body
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Nascent transcript in proventriculus Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva

Adult

Area	Images	Terms

Comments

Low probe on gel Borderline between 'not expressed' and 'low expression'

Salivary glands, probably non-specific.

Borderline between 'not expressed' and 'low expression'

Probe RE26705 Links: Permalink

Genes: CG5873

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Yolk cortex exclusion Pole cell exclusion Basal exclusion Apical exclusion
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Polarized tracheal localization Segmented pattern Salivary glands Salivary glands lumen Proventriculus Posterior spiracles Specific posterior spiracle pattern Hindgut Midgut hindgut border Head Foregut Fat body Ectoderm Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in hindgut Small Foci in ectoderm Bigger Foci

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel

Very interesting tracheal patterns. Some tracheal segments have a 'hat', a 'shoe' and a 'belt' - very cool!

Probe RE23942 Links: Permalink

Genes: CG7884

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Gap-gene pattern Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Cytoplasmic foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Gap-gene pattern Random blastoderm nuclei
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Dorsal trunk Posterior spiracles Hindgut Specific hindgut area or cells Head Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Low probe on gel.

Probe RE20575 Links: Permalink

Genes: CG9192

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Non-Expressed
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Muscles-embryo Visceral mesoderm Midgut Hindgut Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues

Larva

Area	Images	Terms
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in leg discs
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area Brain lobes - Foci or dots
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Not expressed in lymph glands

Adult

Area	Images	Terms

Comments

Good probe on gel.

Probe RE31492 Links: Permalink

Genes: vari

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Apical exclusion Cytoplasmic foci Basal localization Basal enrichment
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Segmented pattern Proventriculus Hindgut Head Head epidermis Foregut Esophagus Ectoderm Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small foci in Proventriculus Small Foci in hindgut Small Foci in ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - Nuclear Midgut - Subnuclear Midgut - foci Specific midgut cells Nascent transcript in midgut Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Hindgut allover Hindgut - Midgut border Expressed in gastric caeca Subcellular localization in gastric caeca Perinuclear gastric caeca Half moon perinuclear gastric caeca Gastric caeca - nuclear localization Gastric caeca - subnuclear localization Gastric caeca allover

Adult

Area	Images	Terms

Comments

Good probe on gel.

Link added manually

Probe RE09982 Links: Permalink

Genes: CG9503

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Non-Expressed Degraded Degraded completely stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Tracheal 'podia' Specific tracheal branches Dorsal trunk Dorsal trunk foci Ectoderm CNS Ventral nerve cord Foci - VNC Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in ectoderm Small Foci in CNS Small Foci in amnioserosa Cell extensions or 'podia'

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues Nervous system Not expressed in nervous system
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Expressed in heart
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Not expressed in lymph glands

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Expression level-TE Expressed Low - TE Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Early elongating spermatids-TE Cytoplasmic in early elongating spermatids Cyst cells -TE Head cyst cell - TE Cytoplasmic - Head cyst cell Distal tip region-TE Expressed in distal tip region - TE Seminal Vesicle Expression level-SV Not detected - SV Ejaculatory Duct Expression level-ED Expressed Low - ED Subcellularly localized - ED Lumenal-ED Sleeve - ED Foci - ED Scattered Foci - ED Cell type - ED Cytoplasmic-ED Apical Enrichment-ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Low - AG Cell type - AG Striated muscle cells-AG Secondary cells - AG Cytoplasmic-SC Main cells -AG Cytoplasmic-MC Clusters - AG Basal Enrichment - MC

Comments

Good probe on gel. Allover with some small foci.

Probe CG9812-RB Links: Permalink

Genes: CG9812

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Non-Expressed
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Posterior spiracles Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Apical enrichment Apical trachea

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

-performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript

-is reported by BDGP in situ database to be expressed in the entire dorsal trunk and parts of the visceral branches of the tracheal system but this particular experiment was only able to detect expression in the most immediate tracheal dorsal trunk adjacent to the posterior spiracles

Probe LP02257 Links: Permalink

Genes: sona

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Salivary glands Proventriculus Posterior spiracles Head Esophagus Ectoderm Blastoderm nuclei Localized

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel.

Salivary glands, probably non-specific.

Probe RE28434 Links: Permalink

Genes: CG18217 CR18217

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Exclusionary patterns Apical exclusion Basal localization Basal enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Midgut Hindgut Foregut Fat body Ectoderm CNS Ventral nerve cord Foci - VNC Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in midgut Small Foci in hindgut Small Foci in foregut Small Foci in fat body Small Foci in ectoderm Small Foci in CNS Small Foci in Brain

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules few foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Particular hindgut area
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Borderline between 'not expressed' and 'low expression'
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - membrane Expressed in salivary glands Salivary glands allover
tracheal system		3rd Instar Larval Tissues Tracheal System Expressed in tracheal tubes
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Cytoplasmic foci in spermatogonium - TE Spermatocytes -TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Cytoplasmic foci in round spermatids - TE Individualizing Spermatids - TE Cytoplasmic in individualizing spermatids - TE Cytoplasmic foci in individualizing spermatids - TE Early elongating spermatids-TE Cytoplasmic in early elongating spermatids Cyst cells -TE Tail cyst cell - TE Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Cytoplasmic - cyst cell Subcellularly localized - TE Expression level-TE Expressed High - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Medium expression in tip region Cytoplasmic Seminal Vesicle Subcellularly localized - SV Expression level-SV Expressed Medium - SV Cell type - SV Sperm Tail Decor - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Anterior ejaculatory duct papilla-ED Cytoplasmic in anterior ejaculatory papilla-ED Expression level-ED Expressed High - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Diffuse localization-ED Apical Enrichment-ED Accessory Gland Subcellularly localized - AG Lumenal - AG Diffuse foci-AG Expression level-AG Expressed High - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Main cells -AG Cytoplasmic-MC Cytoplasmic Foci - AG

Comments

Good probe on gel.

Probe SD09455 Links: Permalink

Genes: CG31044 CR31044

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Nuclei-associated localization Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Ectoderm CNS Ventral nerve cord Nuclear - VNC Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in tracheal nuceli Nuclear in CNS Accumulation nuclear subregion Foci Small Foci Small Foci in amnioserosa Bigger Foci

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Nascent transcript in salivary gland Expressed in salivary glands
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Specific testis expression localization Larval testis foci
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Cytoplasmic foci in spermatogonium - TE Spermatocytes -TE Cytoplasmic in Spermatocytes - TE Cytoplasmic puncta in spermatocyte-TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Cytoplasmic foci in round spermatids - TE Individualizing Spermatids - TE Waste Bag - TE Cytoplasmic in individualizing spermatids - TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Sperm Tail Decoration-TCC associated Retracting/Coiling sperm tail-TCC associated Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Cytoplasmic - Tail cyst cell Cytoplasmic - cyst cell Subcellularly localized - TE Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Early cyst cell Cytoplasmic in early cyst cell Clusters in early cyst cell-TE Cyst stem cell Cytoplasmic in cyst stem cell Higher expression in tip region Cytoplasmic Seminal Vesicle Subcellularly localized - SV Expression level-SV Expressed Low - SV Cell type - SV Sperm Tail Decor - SV Cytoplasmic-SV Ejaculatory Duct Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Diffuse localization-ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Main cells -AG Cytoplasmic-MC

Comments

Re-annotated

Probe LD06565 Links: Permalink

Genes: CR44898 robo2

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Membrane associated Exclusionary patterns Pole cell exclusion Apical exclusion Apical localization Apical enrichment Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Dorsal trunk Dorsal trunk foci Head CNS Ventral nerve cord Foci - VNC Brain Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in head Small Foci in CNS

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Cytoplasmic foci-fat body Expressed in fat body
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Optic lobe Brain lobes - Foci or dots Borderline between 'not expressed' and 'low expression'

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatocytes -TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Individualizing Spermatids - TE Waste Bag - TE Subcellularly localized - TE Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Higher expression in tip region Cytoplasmic Seminal Vesicle Expression level-SV Not Expressed - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Inner lumen - ED Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Diffuse localization-ED Basal Enrichment- ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Low - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Cytoplasmic foci-SC Main cells -AG Cytoplasmic-MC Cytoplasmic Foci - AG

Comments

Good probe on gel

Re-annotated

Probe RE05926 Links: Permalink

Genes: Cad96Ca

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Other subcellular pattern Cytoplasmic foci Embryonic localization patterns Yolk cortex localization Yolk cortex enrichment
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Cytoplasmic foci Apical localization Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Salivary glands Salivary glands lumen Proventriculus Posterior spiracles Pharynx Ectoderm Polarized Ectoderm Blastoderm nuclei Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in ectoderm Bigger Foci Apical enrichment Apical ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel. RNA enriched at the early embryo cortex - very interesting.

Salivary glands, probably non-specific.

Probe CrebA-RA Links: Permalink

Genes: CrebA

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Non-Expressed
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Yolk Nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Weak expression (>41ms exp) Localized Yolk plasm localization Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Expression in API Expression in amnioserosa Dorsal blastoderm nuclei Medium expression (21-40ms exp) Localized Yolk plasm localization Embryonic localization patterns Ventral localization Dorsal localization
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Tracheal lumen Specific tracheal branches Dorsal trunk Segmented pattern Salivary glands Proventriculus Pharynx Head Head epidermis Foregut Ectoderm Amnioserosa Amnioserosa edges Medium expression (21-40ms exp)

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript

-diverse ectoderm derived structures such as the salivary gland (and the salivary primordium), amnioserosa, proventriculus, tracheal pits, and epidermis

-prior reported expression of hemocytes were not visible in this experiment

Probe LD19584 Links: Permalink

Genes: Dl

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in ectoderm
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Dorsal trunk Dorsal trunk foci Segmented pattern Salivary glands Salivary glands lumen Proventriculus PNS Neuroblasts Polarized Neuroblasts Midgut Midgut subset Hindgut Head Fat body Ectoderm Polarized Ectoderm CNS Ventral nerve cord Blastoderm nuclei Atrium Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around ectoderm nuclei Foci Small Foci Small Foci in tracheal cells Small foci in Proventriculus Small Foci in PNS Small Foci in hindgut Small Foci in head Small Foci in ectoderm Small Foci in CNS Cell junction associated

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing discs particular pattern Wing disc few cells - dots Expressed in eye disc Eye disc few cells - dots
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Ventral nerve cord - allover Localized to axons Expressed in brain lobes Brain lobes allover
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' Lymph Glands Expressed in lymph glands Lymph glands all over

Adult

Area	Images	Terms

Comments

Good probe on gel.

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions'

Probe GM08552 Links: Permalink

Genes: F-element{}1209 flam

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Ubiquitous
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp)
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Tracheal system Tracheal lumen Tracheal 'podia' Specific tracheal branches Dorsal trunk Dorsal trunk foci Salivary glands Proventriculus Midgut Midgut subset Hindgut Head Ectoderm Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in midgut Small Foci in hindgut Small Foci in ectoderm Cell extensions or 'podia'

Larva

Area	Images	Terms
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Proventriculus allover Expressed in midgut Subcellular localization in midgut Midgut - Nuclear Midgut - Subnuclear Specific midgut cells Nascent transcript in midgut Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - Nuclear in Malpighian tubules - Subnuclear in Malpighian tubules - foci Nascent transcript in malpighian tubules Expressed in hindgut Subcellular localization in hindgut Nuclear in hindgut Hindgut - subnuclear localization Hindgut perinuclear Nascent transcript in hindgut
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs few cells - dots Borderline between 'not expressed' and 'low expression'
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - Perinuclear Salivary gland - nuclear dots Salivary gland - Cytoplasmic foci Cytoplasmic foci in Salivary Gland Lumen Salivary gland - apical Salivary gland - lumen Nascent transcript in salivary gland Expressed in salivary glands

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatocytes -TE Nuclear in Spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Nuclear in round spermatids-TE Cytoplasmic in round spermatids-TE Individualizing Spermatids - TE Cystic bulge - TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Sperm Tail Decoration-TCC associated Retracting/Coiling sperm tail-TCC associated Retracting/Coiling Tail Cyst Cell - TE Retracting/Coiling Tail Cyst Cell Cytoplasm - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Nuclear Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Lower expression in tip region Cytoplasmic Seminal Vesicle Cell type - SV Sperm Tail Decor - SV Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Expression level-SV Expressed Low - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Inner lumen - ED Anterior ejaculatory duct papilla-ED Cytoplasmic in anterior ejaculatory papilla-ED Apical membrane enriched-Anterior ejaculatory papilla Expression level-ED Expressed High - ED Cell type - ED Cytoplasmic-ED Membrane enriched - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG ECM like pattern-AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Main cells -AG Cytoplasmic-MC Apical Enrichment - MC

Comments

The probe recognizes the transposon and also flamenco and maybe some other areas in the genome that contain a highly homologous sequence.

Probe GH15080 Links: Permalink

Genes: Fas2

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Degraded Degraded partially stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp)
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Dorsal trunk Polarized tracheal localization Segmented pattern Ring gland Proventriculus Posterior spiracles PNS Malpighian tubules Hindgut Specific hindgut area or cells Head Foregut Fat body Fat body- specific region Fat body polarized Dorsal vessel CNS Ventral nerve cord Foci - VNC Axons Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around CNS nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in PNS Small Foci in hindgut Small Foci in fat body Small Foci in CNS Cell extensions or 'podia'

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Borderline between 'not expressed' and 'low expression'

Good probe.

Specific tracheal areas and branches.

Probe LD19609 Links: Permalink

Genes: ImpE2

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Pole plasm excluded
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Posterior localization Embryonic localization patterns Exclusionary patterns Pole cell exclusion Anterior localization
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Posterior localization Anterior localization
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Segmented pattern Midgut Midgut subset Head Ectoderm Polarized Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around tracheal nuclei Perinuclear around ectoderm nuclei

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
imaginal discs		3rd Instar Larval Tissues Imaginal discs Possible peripodial membrane expression Expressed in leg discs
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva Specific ovary expression localization Ovary nuclear 'dot' Ovary foci Expressed in Ovary
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover

Adult

Area	Images	Terms

Comments

Good probe on gel.

Sub and peri nuclear with apical enrichment. Anterior cells only. Very specific and interesting.

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions'

Probe RE13893 Links: Permalink

Genes: Osi20

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Basal localization Basal enrichment Degraded Degraded partially stage 7 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded completely stage 9 Degradation maternal transcripts
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Salivary glands Salivary glands lumen Localized

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Tracheal system.

Good probe on gel.

Probe LD24835 Links: Permalink

Genes: RhoBTB

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Medium expression (21-40ms exp) Maternal
stage 4-5		Stage 4-5 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Basal localization Basal enrichment
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 7 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Ubiquitous Maternal
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Tracheal system Tracheal lumen Specific tracheal branches Dorsal trunk Blastoderm nuclei Localized Subcellular localization patterns Foci

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Need more images to better tell the story.

Good probe on gel.

Probe RE42104 Links: Permalink

Genes: Ser

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Segmented pattern Posterior spiracles PNS CNS Ventral nerve cord Foci - VNC Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Membrane associated Foci Small Foci Small Foci in tracheal cells Small Foci in PNS Small Foci in CNS Small Foci in Brain

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Re-annotated

Atrium membrane

Probe SD02116 Links: Permalink

Genes: aft

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Non-Expressed Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Salivary glands Posterior spiracles Midgut Hindgut Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in salivary glands Small Foci in midgut

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel.

Probe RE04051 Links: Permalink

Genes: dpa

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Segmented pattern PNS Neuroblasts Midgut Hindgut Head Ectoderm Polarized Ectoderm CNS Ventral nerve cord Perinuclear VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around CNS nuclei Perinuclear 'rings' Foci Small Foci Small Foci in tracheal cells Small Foci in midgut Small Foci in hindgut Small Foci in head Small Foci in CNS Small Foci in Brain Apical enrichment Apical ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Allover with foci.

Good probe on gel.

Salivary glands, probably non-specific. Interesting patterns.

Probe RE20611 Links: Permalink

Genes: dpp

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Apical localization Apical enrichment Apical clusters Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Apical localization Apical enrichment Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Tracheal lumen Specific tracheal branches Dorsal trunk Neuroblasts Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Midgut Hindgut Head Ectoderm Blastoderm nuclei Atrium Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in other tissues Small Foci in midgut Small Foci in hindgut Small Foci in head Small Foci in ectoderm Small Foci in amnioserosa Apical enrichment Apical enriched hindgut

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Perinuclear localization Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Nascent transcript in proventriculus Expressed in midgut Subcellular localization in midgut Midgut - Nuclear Midgut - Subnuclear Nascent transcript in midgut Midgut allover Expressed in hindgut Hindgut - Midgut border Expressed in gastric caeca Gastric caeca allover
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area
ovary and testis		3rd Instar Larval Tissues Larval Ovary and Testis-Larva Ovary allover Expressed in Ovary
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva

Adult

Area	Images	Terms

Comments

Clear patches of cells close to the invaginating tracheal placodes.

Good probe on gel. RNA is allover, with some small but clear foci.

Probe GH03529 Links: Permalink

Genes: drpr

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Embryonic localization patterns Yolk plasm localization Yolk plasm enrichment
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Nuclei-associated localization Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Perinuclear around yolk nuclei Inside Yolk nuclei Tracheal system Tracheal pits Specific tracheal branches Dorsal trunk Dorsal trunk foci Salivary glands Salivary glands lumen Posterior spiracles Fat body Ectoderm CNS Ventral nerve cord Foci - VNC Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Perinuclear localization Nuclei-associated localization Accumulation nuclear subregion Foci Small Foci Small Foci in tracheal cells Small Foci in midgut Small Foci in fat body Small Foci in ectoderm Small Foci in CNS Small Foci in amnioserosa

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Muscle. Partly nascent, partly sub-nuclear.

Salivary glands, probably non-specific.

Probe LD43328 Links: Permalink

Genes: flz

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Cytoplasmic foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Weak expression (>41ms exp)
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Segmented pattern Salivary glands Salivary glands lumen Posterior spiracles Specific posterior spiracle pattern Pharynx Hindgut Foregut Fat body Esophagus Ectoderm Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in hindgut Small Foci in foregut Small Foci in ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Low expression.

All over with small foci. Low expression.

Low, low probe' All over with small foci. Low expression.

Salivary glands, probably non-specific.

Probe LD19710 Links: Permalink

Genes: ft

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei
stage 8-9		Stage 8-9 Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Dorsal trunk Dorsal trunk foci Segmented pattern Salivary glands Salivary glands lumen Posterior spiracles Hindgut Specific hindgut area or cells Ectoderm Polarized Ectoderm Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in hindgut Small Foci in ectoderm Apical enrichment Apical trachea Apical enriched hindgut Apical ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs particular pattern Leg discs few cells - dots Leg discs allover
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Ventral nerve cord - allover Expressed in brain lobes Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
ovary and testis		3rd Instar Larval Tissues Larval Ovary and Testis-Larva Not expressed in larval ovary
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression'

Adult

Area	Images	Terms

Comments

Borderline between 'not expressed' and 'low expression'

Good, strong probe on gel.

Salivary glands, probably non-specific.

Probe LD24749 Links: Permalink

Genes: jumu

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Terminal-gene pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization Membrane associated Exclusionary patterns Pole cell exclusion Dorsal localization Anterior localization Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Salivary glands Salivary glands lumen Posterior spiracles PNS Ectoderm Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in CNS

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel

It may be membrane associated... a closer look is required to determine this Interesting zygotic expression.

Probe LD32943 Links: Permalink

Genes: nop5

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Posterior spiracles Pharynx Muscles-embryo Muscles - subregion Midgut Foregut Esophagus Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in Pharynx Small Foci in foregut Small Foci in ectoderm Cell junction associated

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel, a bit low.

Probe LD36528 Links: Permalink

Genes: sug

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Pole plasm excluded
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Ubiquitous
stage 8-9		Stage 8-9 Expressed Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Dorsal trunk Salivary glands Salivary glands lumen Muscles-embryo Visceral mesoderm Muscles - subregion Midgut Midgut subset Hindgut Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Basal enrichment Basal in midgut

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Posterior midgut, basal enriched.

Good probe on gel.

Result Summary

Probe CG12519 Links: Permalink Annotated by Steven

Genes: CG12519

Embryo

Larva

Adult

Comments

Probe GH05754 Links: Permalink Annotated by Ronit

Genes: Ance-5

Embryo

Larva

Adult

Comments

Probe RE18408 Links: Permalink Annotated by Ronit

Genes: Best2

Embryo

Larva

Adult

Comments

Probe RE29777 Links: Permalink Annotated by Ronit

Genes: CG10936

Embryo

Larva

Adult

Comments

Probe CG1440-RB Links: Permalink Annotated by Steven

Genes: CG1440

Embryo

Larva

Adult

Comments

Probe LD43683 Links: Permalink Annotated by Ronit

Genes: obst-A

Embryo

Larva

Adult

Comments

Probe GH01149 Links: Permalink Annotated by Ronit

Genes: CG17274

Embryo

Larva

Adult

Comments

Probe LD24702 Links: Permalink Annotated by Ronit

Genes: CG17286

Embryo

Larva

Adult

Comments

Probe LD24679 Links: Permalink Annotated by Ronit

Genes: CG2767

Embryo

Larva

Adult

Comments

Probe RE11181 Links: Permalink 3rd Instar Larval Tissues Annotated by Indrani Annotated by Ronit

Genes: CG31121

Embryo

Larva

Adult

Comments

Probe RE24343 Links: Permalink 3rd Instar Larval Tissues Annotated by Ronit

Genes: CG32843

Embryo

Larva

Adult

Comments

Probe RE10255 Links: Permalink 3rd Instar Larval Tissues Annotated by Indrani Annotated by Ronit Annotated by Zhantao

Genes: CG33129

Embryo

Larva

Adult

Comments

Probe LD16982 Links: Permalink Annotated by Eric Annotated by Ronit

Genes: CG4040

Embryo

Larva

Adult

Comments

Probe LP07955 Links: Permalink Annotated by Ronit

Probe CG12519 Links: Permalink

Probe GH05754 Links: Permalink

Probe RE18408 Links: Permalink

Probe RE29777 Links: Permalink

Probe CG1440-RB Links: Permalink

Probe LD43683 Links: Permalink

Probe GH01149 Links: Permalink

Probe LD24702 Links: Permalink

Probe LD24679 Links: Permalink

Probe RE11181 Links: Permalink

Probe RE24343 Links: Permalink

Probe RE10255 Links: Permalink

Probe LD16982 Links: Permalink

Probe LP07955 Links: Permalink

Probe GH02976 Links: Permalink

Probe LP10922 Links: Permalink

Probe GH10366 Links: Permalink

Probe RE19838 Links: Permalink

Probe RE26705 Links: Permalink

Probe RE23942 Links: Permalink

Probe RE20575 Links: Permalink

Probe RE31492 Links: Permalink

Probe RE09982 Links: Permalink

Probe CG9812-RB Links: Permalink

Probe LP02257 Links: Permalink