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| Number of genes | 35 | |
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| Subcell gonads (maybe). |
| Low probe on gel. Good in situ. |
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| Good probe, a bit weak. |
| small foci. |
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| Anterior and posterior midgut |
| Good probe, nice in situ. |
| Anterior and posterior midgut |
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| Good probe on gel. |
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| Good probe on gel- strong. |
| Salivary glands, probably non-specific. |
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| Localized to pole cells, and pole plasm. |
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| Polarized perinuclear with some 'rings' as well at the pole cells. Apical enriched anterior. |
| Polarized perinuclear |
| Interesting pattern. |
| Good probe on gel. Strong. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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| The probe looks good on gel. |
| Dynamic expression. Salivary glands, probably non-specific |
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| Low probe on gel. Nice insitu. Interesting patterns of localization. |
| Asymmetric perinuclear localization in pole cells. |
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| Good probe. |
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| Good probe on gel. |
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| Salivary glands, muscles, ectoderm stripes, head. |
| Pole cells. |
| The probe looks good on gel Enriched at the pole plasm by stage 3. |
| Pole cells. |
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| Cytoplasmic foci around pole cells. |
| Pole plasm and pole buds enriched. |
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| Good probe on gel Goes from all-over to 'posterior enriched'. |
| Anterior zygotic and posterior maternal expression. |
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| Good probe. |
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| Re-annotated |
| Small 'foci'. |
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| Home-made probe to exon 3. Good probe on gel. |
| Interesting patterns! |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions |
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| Good probe on gel Pole plasm enriched, very strong expression (3ms photos). Mild gradient Posterior to Anterior. |
| Posterior to anterior gradient. |
| Very specific and dynamic expression. Perinuclear. Salivary glands, probably non-specific. |
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| Very low probe on gel. Posterior enriched transcript. |
| Salivary glands, probably non-specific. |
| All over, with low pole cell enrichment. |
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| Amnioserosa cells, cytoplasmic foci. And prinuclear around specific yolk nuclei. |
| Good probe, nice insitu. |
| Nuclear localization in ventral nerve cord. Salivary glands, probably non-specific. |
| Amnioserosa foci, peri-pole cells, head. |
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| Important note: this data was produced with probe 'RE23969'. Consistent with BDGP in situ data Good probe on gel. |
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| The probe is good but low' Good insitu ------ Intersting expression. |
| Partly nascent, partly sub-nuclear. |
| Partly nascent, partly sub-nuclear. |
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| Salivary glands, probably non-specific. |
| Good probe on gel. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -interesting subcellular localization patterns in the pole/germ cells; makes a great marker for tracking the development of germ line cells throughout the entirety of embryogenesis |
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| Again, perinuclear pole cell asymmetric expression. |
| Pole cell asymmetric expression. I see small 'dots' around the pole cells, stronger in one 1/2 of the pole nuclei. |
| Good probe on gel |
| Posterior localization, even in unfertilized eggs. RNA accumulates asymmetrically around the pole buds with a 'fiber-like' texture. When nuclei divide, the RNA will stay in the nuclei that are closer to the posterior end. Very interesting! |
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| Good probe Very interesting localization! |
| Nice! |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -neat expression patterns in early embryos and early germ band elongation stages; expression is no longer detectable after stage 9-10 (fits modENCODE RNA-seq data well) |