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Result Summary
| Number of genes | 28 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Malpighian tubules Head Head epidermis Ectoderm Anal pad |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Cell division apparatus Other cell division pattern Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Cytoplasmic foci Cell division apparatus Other cell division pattern Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Posterior spiracles Specific posterior spiracle pattern Pharynx Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in Pharynx Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Very low probe on gel. |
| Very interesting patterns. |
| Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Pole cell nuclei Blastoderm nuclei All blastoderm nuclei Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei Maternal Localized Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Maternal Localized Germ cell localization Germ cell enrichment Embryonic localization patterns |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Probe CG16815-RB Links: Permalink
Genes: CG16815
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Single stripe Posterior blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Posterior localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Exclusionary patterns Yolk cortex exclusion Pole cell exclusion Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Degraded Degraded partially stage 7 Degraded partially stage 6 |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Degraded Degraded partially stage 8 Degraded completely stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Head Head epidermis Ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
Probe CG31131-ex Links: Permalink
Genes: CG31131
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Posterior localization Embryonic localization patterns Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Weak expression (>41ms exp) Ubiquitous Localized Embryonic localization patterns Posterior localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Ectoderm Weak expression (>41ms exp) Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
| -modENCODE RNAseq data on FlyBase shows low/no expression, however, we observed strong expression patterns throughout development |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization RNA islands Pole plasm Anterior localization Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Basal localization Basal enrichment Apical localization Apical enrichment Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cell junction associated Basal localization Basal enrichment Apical localization Apical enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Pharynx Muscles-embryo Visceral mesoderm Muscles - subregion Midgut Midgut subset Hindgut Fat body Ectoderm Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around pharynx nuclei Perinuclear around muscle nuclei Perinuclear around midgut nuclei Basal enrichment Basal in midgut Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Weak probe, good insitu. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei All blastoderm nuclei Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Cell division apparatus Other cell division pattern Basal localization Basal enrichment Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in all nuclei Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in ectoderm Expression in amnioserosa Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Ectoderm CNS Ventral nerve cord Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Nuclear in CNS Nuclear in amnioserosa |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Midgut allover Expressed in hindgut Hindgut allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Cytoplasmic foci Expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Distal tip region-TE Not Expressed in distal tip region -TE Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Individualizing Spermatids - TE Waste Bag - TE Cytoplasmic in individualizing spermatids - TE Cyst cells -TE Tail cyst cell - TE Retracting/Coiling Tail Cyst Cell - TE Retracting/Coiling Tail Cyst Cell Cytoplasm - TE Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed High - TE Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Expression level-SV Expressed High-SV Cell type - SV Cytoplasmic-SV Membrane enriched - SV Cytoplasmic Foci - SV Ejaculatory Duct Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Cytoplasmic foci-SC Main cells -AG Cytoplasmic-MC |
Comments
| All nuclei. Strong in the Amnioserosa. |
| Enriched in the amnioserosa, the central nervous system, the ventral midline, and maybe the tracheal pits. |
| Anterior to posterior gradient Subnuclear localization. |
| The probe is low, but good. Nice insitu data!!! |
Probe LP10071 Links: Permalink
Genes: Ef1alpha48D
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Ventral localization Posterior localization Other subcellular pattern Anterior localization Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Strong expression (1-20ms exp) Localized Subcellular localization patterns Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Strong expression (1-20ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Ubiquitous Localized Subcellular localization patterns Membrane associated |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus - Few foci Particular proventriculus area Expressed in midgut Subcellular localization in midgut Midgut - foci Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc few cells - dots Eye disc basal Eye disc - center round cells Expressed in antennal disc Antennal disc few cells - dots Antennal disc - center round cells |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Maybe membranes... need better data. |
Probe Hsromega-ex Links: Permalink
Genes: Hsr-omega
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Yolk nuclei Subset yolk nuclei Ventral yolk nuclei Posterior yolk nuclei Anterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Ventral localization Posterior localization Exclusionary patterns Pole cell exclusion Basal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Localized Posterior localization Embryonic localization patterns Ventral localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Strong expression (1-20ms exp) Localized Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Midgut Head Head epidermis Ectoderm CNS Ventral nerve cord Brain Amnioserosa Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
| -lcnRNA |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole plasm Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Posterior localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Cell junction associated Basal localization Basal enrichment Apical localization Apical enrichment Anterior localization Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cell junction associated Apical localization Apical enrichment Diffuse apical localization Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in neuroblasts Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Other subcellular pattern Cytoplasmic foci Germ cell localization Germ cell enrichment Embryonic localization patterns Ventral localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern Salivary glands Posterior spiracles PNS Neuroblasts Polarized Neuroblasts Midgut Midgut subset Head CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around midgut nuclei Perinuclear around CNS nuclei Perinuclear 'rings' Foci Small Foci Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Perinuclear localization Half moon perinuclear Expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Expressed in midgut Subcellular localization in midgut Midgut - Perinuclear Midgut - foci Expressed in gastric caeca Nascent transcript in gastric caeca Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs few cells - dots Leg discs - outside Expressed in eye disc Eye disc basal Eye disc - center round cells Expressed in antennal disc Antennal disc - localized outside Antennal disc - center round cells |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Perinuclear Salivary gland - Half moon perinuclear Nascent transcript in salivary gland Expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Perinuclear Ring gland - Cytoplasmic foci Nascent transcript in ring gland Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel Goes from all-over to 'posterior enriched'. |
| Anterior zygotic and posterior maternal expression. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Posterior localization Embryonic localization patterns Exclusionary patterns Pole cell exclusion Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Posterior localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Segmented pattern Midgut Midgut subset Head Ectoderm Polarized Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around tracheal nuclei Perinuclear around ectoderm nuclei |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Possible peripodial membrane expression Expressed in leg discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva Specific ovary expression localization Ovary nuclear 'dot' Ovary foci Expressed in Ovary |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Sub and peri nuclear with apical enrichment. Anterior cells only. Very specific and interesting. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Embryonic localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Posterior spiracles Muscles-embryo Muscles - subregion Maxillary complex Head Head epidermis Ectoderm Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -There is no entry for this gene on BDGP, and modENCODE RNAseq data on FlyBase indicates that it is not expressed in the embryo. However, we observed obvious patterns during embryogenesis. Additionally, FlyBase does not have any in-depth characterizations of this gene. |
| -Osi21 does not overlap with any other genes, ruling out the possibility of the targeting of alternative transcripts. BLAST also confirms the specificity of our probes to Osi21. Additionally, no other genes for which probes were designed for on this plate show patterns such as those observed here, ruling out the possibility of cross-contamination during probe preparation. |
| -This may be a result of Osi21's poor characterization or incorrect RNAseq data |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Yolk plasm exclusion Pole cell exclusion Cytoplasmic foci Apical localization Apical enrichment Discrete apical foci Diffuse apical localization Anterior localization Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in API Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Note: this in situ was done twice with the same result The next gene on the plate has a similar expression pattern |
| Nice anterior to posterior gradient. Apical expression. Cytoplasmic foci also in a gradient anterior to posterior. Very interesting. |
Probe LD29301 Links: Permalink
Genes: Su(var)2-HP2
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Anterior localization Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Salivary glands Proventriculus Head Fat body Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| re-annotated |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Head Head epidermis Ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Terminal-gene pattern Gap-gene pattern Localized Subcellular localization patterns Ventral localization Posterior localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Membrane associated Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Exclusionary patterns Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Other subcellular pattern Nuclei-associated localization Intranuclear accumulation |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Ring gland Proventriculus PNS Neuroblasts Polarized Neuroblasts Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Muscles - subregion Midgut Midgut subset Midgut ICPs Hindgut Head Fat body Ectoderm Polarized Ectoderm CNS Ventral nerve cord Nuclear - VNC Axons Brain Blastoderm nuclei Atrium Anal pad Localized Subcellular localization patterns Perinuclear localization Nuclei-associated localization Nuclear in PNS Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus Cell extensions or 'podia' Apical enrichment Apical enriched midgut Apical enriched hindgut |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel Maybe some small foci. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Segmented pattern Terminal-gene pattern Posterior cortical nuclei Anterior cortical nuclei Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Yolk cortex exclusion Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Posterior localization Exclusionary patterns Pole cell exclusion Dorsal localization Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Other subcellular pattern Nuclei-associated localization Cell junction associated Apical localization Apical enrichment Apical Localization Neuroblasts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Proventriculus |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Lymph Glands Not expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Degrades. |
| Very interesting. |
| Perinuclear around neuroblasts. |
| Anterior dorsal and posterior dorsal enriched and apically enriched on these cells as well. |
| Good probe on gel. Amazing in situ. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Pronucleus-associated Anterior localization Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern All blastoderm nuclei Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Apical localization Apical enrichment Diffuse apical localization Local terms for zygotic transcripts Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Localized Subcellular localization patterns Apical localization Apical enrichment Diffuse apical localization Apical Localization Neuroblasts Local terms for zygotic transcripts Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Neuroblasts Polarized Neuroblasts CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in CNS Apical enrichment Apical in other tissues |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Re-annotated |
| Neuroblasts |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Anterior localization Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland |
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Anterior localization Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization Exclusionary patterns Yolk plasm exclusion Basal exclusion Apical localization Apical enrichment Diffuse apical localization Apical clusters Anterior localization Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in ectoderm Expression in API Localized |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Ectoderm CNS Ventral nerve cord Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Foci Small Foci Small Foci in other tissues Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Central nervous system |
| Anterior gradient. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Anterior localization Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Apical clusters Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Strong expression (1-20ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in API Expression in amnioserosa Strong expression (1-20ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Muscles-embryo Ectoderm CNS Ventral nerve cord Axons Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Apical, anterior to posterior gradient. |
| Anterior to posterior gradient! |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Terminal-gene pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization Membrane associated Exclusionary patterns Pole cell exclusion Dorsal localization Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Salivary glands Salivary glands lumen Posterior spiracles PNS Ectoderm Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel |
| It may be membrane associated... a closer look is required to determine this Interesting zygotic expression. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Strong expression (1-20ms exp) Localized Subcellular localization patterns Yolk plasm localization Ventral localization Dorsal localization Cytoplasmic foci Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Expression in ectoderm Strong expression (1-20ms exp) Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Strong expression (1-20ms exp) Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Germ band localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Hindgut Specific hindgut area or cells Head Head epidermis Ectoderm Anal pad Ring around anal pad Strong expression (1-20ms exp) |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization RNA islands Pole plasm Pole buds Anterior localization Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei All blastoderm nuclei Maternal Localized Subcellular localization patterns Ventral localization Exclusionary patterns Pole cell exclusion Anterior localization Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern All blastoderm nuclei Localized Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole plasm Anterior localization Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Random blastoderm nuclei Dorsal blastoderm nuclei All blastoderm nuclei Transiently widespread Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Anterior localization Embryonic localization patterns Degraded Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Re-annotated |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Anterior localization Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Membrane associated Exclusionary patterns Pole cell exclusion Cell junction associated Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in API Weak expression (>41ms exp) Localized |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Expression in mesoderm Expression in ectoderm Weak expression (>41ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Head CNS Blastoderm nuclei Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Anterior ot posterior gradient and apical to apical/lateral enrichment in epithelial cells. |
| Anterior to posterior gradient! |
| Segmented zygotic expression. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Ventral localization Posterior localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Yolk plasm exclusion Pole cell exclusion Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Expression in ectoderm Anterior blastoderm nuclei Localized Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Yolk cortex exclusion Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Posterior localization Embryonic localization patterns Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in mesoderm Expression in ectoderm Expression in API Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Ventral localization Germ band localization Dorsal localization Anterior localization Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Muscles - subregion Hindgut Head Foregut Esophagus Ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around muscle nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -retested using HCR-FISH 2025 for full coverage of late stage expression patterns |
| -performed using 3 probe pairs (HCR-FISH) against the RB isoform |
| -really interesting patterns! |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Ubiquitous Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment Ventral localization Posterior localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Basal localization Apical localization Apical enrichment Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Expression in API Dorsal blastoderm nuclei Localized Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Cytoplasmic foci Pole cell localization Pole cell enrichment Embryonic localization patterns Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Expression in API Expression in amnioserosa Dorsal blastoderm nuclei Localized Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Cytoplasmic foci Basal localization Germ cell localization Germ cell enrichment Embryonic localization patterns Dorsal localization Degraded Degraded partially stage 9 |
| stage 10-17 |
Stage 10-17 Non-Expressed |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -neat expression patterns in early embryos and early germ band elongation stages; expression is no longer detectable after stage 9-10 (fits modENCODE RNA-seq data well) |