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| Number of genes | 40 | |
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| Low probe on gel - still good in situ! |
| Good example of 'muscle podia' ! |
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| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -appears ubiquitously throughout development with some mesoectodermal enrichment during the germ band elongation phases (appears as stripes) |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -Based on previous experiments and literature, ventral spots observed at stages 10 and 11 are likely neuroblast or other neural precursors |
| -BDGP did not observe this pattern, and there is no characterization of this gene on FlyBase |
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| Polarized perinuclear with some 'rings' as well at the pole cells. Apical enriched anterior. |
| Polarized perinuclear |
| Interesting pattern. |
| Good probe on gel. Strong. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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| Nice insitu. No specific sub-cellular localization. |
| Good probe on gel. |
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| Need more images to get the full story. In situ done in 2006, before we started screening late stage embryos. |
| Re-annotated |
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| -performed via HCR-FISH using 3 probe sets targeting the RC isoform transcript |
| -the gene is a lncRNA with quite interesting patterns in the CNS (brain and VNC) |
| -seems to be expressed in the anterior transverse furrow, shown in stage 6-7 images |
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| Good probe on gel. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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| Salivary glands, probably non-specific. |
| Low probe on gel. Good in situ. |
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| Important note: These images come from plate AM05 (or RA.304) B12. Same CG number as His3.3A different probe (RE14004) ------- The probe looks good on gel. |
| Salivary glands, probably non-specific. |
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| Low probe, nice insitu... |
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| Ventral nerve cord, midgut, muscles, etc. |
| Posterior gradient, enriched ventrally. Strong expression. |
| Good probe on gel. |
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| Salivary glands, probably non-specific. |
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| Good probe on gel Goes from all-over to 'posterior enriched'. |
| Anterior zygotic and posterior maternal expression. |
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| Re-annotated |
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| Low probe (good in situ hybridization) |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -very weak signal in the PNS |
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| Good probe on gel Maybe some small foci. |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -neat marker for neural precursor formation, appears to not be expressed past stage 13-14 except for 4 small points near the dorsal-posterior region of the ectoderm (possibly tip cells of the Malpighian tubules); clear stomatogastric nervous system primordium expression |
| -images are from two separate experiments under identical conditions for better coverage |
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| Re-annotated |
| Neuroblasts |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
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| Re-annotated 2014 |
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| -performed via HCR-FISH (3 probe sets) |
| -isoform corn-RC transcript was targeted |
| -weak expression in the late embryos that appear as small points along the ectoderm of the each segment (trunk and abdominal) located dorsolateral. |
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| -probes were designed against RB isoform using HCR-FISH probes (3 probe sets) |
| -expression appears ubiquitous at all stages but shows clear CNS enrichment in later stages |
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| Allover with foci. |
| Good probe on gel. |
| Salivary glands, probably non-specific. Interesting patterns. |
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| Clear patches of cells close to the invaginating tracheal placodes. |
| Good probe on gel. RNA is allover, with some small but clear foci. |
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| Low expression. Some 'foci'. |
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| Nice apical localizatin in neuroblasts during and after delamination. |
| Zygotically expressed RNA localizes apically and along the lateral membrane. RNA is likely cell junction associated. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -Somatic muscle pattern observed aligned with BDGP but may alternatively be PNS signal |
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| Low probe on gel. |
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| Small foci. |
| Small foci. |
| Re-annotated |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -expression seen in the stomatogastric sensory primordium, and the anterior and posterior midgut primordium; forms a complex along with ac (CG3796) so should colocalize in neuronal regions |
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| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
| -good marker for the early neuroectoderm |
| -RNAseq data and experiments done by BDGP suggest no maternal expression, however we observe what appears to be maternal expression that borders between expressed and not expressed |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -very diverse and neat patterns at all stages |