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Result Summary
| Number of genes | 46 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 7 Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in neuroblasts Expression in amnioserosa Degraded Degraded completely stage 9 Degradation maternal transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic PNS CNS Ventral nerve cord Axons Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Foci Small Foci Small Foci in PNS Small Foci in CNS Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus - axons |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs few cells - dots Leg discs allover Expressed in eye disc Eye disc photo-receptors Lateral photo-receptor area Eye disc few cells - dots Expressed in antennal disc Antennal disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Localized to axons Expressed in brain lobes Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions |
| Sometimes one larval brain lobe reacts to the in situ hybridization and the other one does not - it may be that one of the reagents was not exposed properly - This in situ may have to be redone in larval brains for absolute confirmation. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Sensory system Salivary glands Posterior spiracles Specific posterior spiracle pattern PNS Hindgut Specific hindgut area or cells Midgut hindgut border Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in other cells Accumulation entire nucleus Foci Small Foci Small Foci in tracheal cells |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern PNS Head Ectoderm Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Foci Small Foci Small Foci in PNS Small Foci in ectoderm Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body Borderline between 'not expressed' and 'low expression' |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes - Foci or dots |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Exclusionary patterns Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Pole cell localization Pole cell enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Germ cell localization Germ cell enrichment Embryonic localization patterns Degraded Degraded partially stage 9 Degradation maternal transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands Salivary glands lumen Posterior spiracles PNS Oenocytes Muscles-embryo Midgut Hindgut Germ cell nuclei Gonads Specific Gonad pattern CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around midgut nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Subcell gonads (maybe). |
| Low probe on gel. Good in situ. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Localized Subcellular localization patterns Membrane associated Basal localization Apical localization |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Membrane associated Basal localization Basal enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic PNS Head CNS Ventral nerve cord Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Foci Small Foci Small Foci in PNS Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe on gel. Beautiful neuronal pattern in embryos! |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands PNS Midgut Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Foci Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Probe is a bit low on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in ectoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Cytoplasmic foci Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands lumen PNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Foci Small Foci Small Foci in PNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Probe looks good on gel. Maybe some small foci... |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in other cells Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Borderline between 'not expressed' and 'low expression' |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Exclusionary patterns Yolk cortex exclusion |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Yolk cortex localization Yolk cortex enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Expression in API Localized Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Exclusionary patterns Apical exclusion Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands PNS Oenocytes Midgut Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Foci Small Foci Small Foci in PNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands PNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Foci Small Foci Small Foci in PNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe, a bit weak. |
| Borderline between 'not expressed' and 'low expression' |
| Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Foci Small Foci Small Foci in PNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Not sure if this is 'specific' expression... |
| Nice nervous system. Salivary glands, probably non-specific. |
| Low probe on gel (the late stage expression shows that the probe works fine) |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 5 Degradation maternal transcripts |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Salivary glands Posterior spiracles Oenocytes Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Cytoplasmic foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern PNS Dorsal vessel CNS Ventral nerve cord Polarized VNC Foci - VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around dorsal vessel nuclei Foci Small Foci Small Foci in PNS Small Foci in dorsal vessel Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Interesting. |
| Low low probe on gel. Nice small foci. |
| Small foci |
| Small foci. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Gap-gene pattern Dorsal blastoderm nuclei Weak expression (>41ms exp) Degraded Degraded completely stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Expression in amnioserosa Dorsal blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands PNS CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Foci Small Foci Small Foci in PNS Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe. |
| Perinuclear. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Non-Expressed |
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Sensory system PNS Oenocytes CNS Ventral nerve cord Foci - VNC Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around CNS nuclei Foci Small Foci Small Foci in PNS Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Sensory system Salivary glands Posterior spiracles PNS Pharynx Hindgut Dorsal vessel Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around pharynx nuclei Nuclei-associated localization Nuclear in PNS Accumulation entire nucleus Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Nuclear Sensory system. Salivary glands, probably non-specific. |
| Small foci. |
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands Posterior spiracles Specific posterior spiracle pattern PNS Head Head epidermis Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Not expressed in larval muscles - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Not expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe on gel. |
| Interesting anterior late embryo expression and sensory neurones. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS CNS Ventral nerve cord Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Maternal Degraded Degraded partially stage 9 Degradation maternal transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS CNS Ventral nerve cord Polarized VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around CNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in ectoderm |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Dorsal trunk Dorsal trunk foci Segmented pattern Salivary glands Salivary glands lumen Proventriculus PNS Neuroblasts Polarized Neuroblasts Midgut Midgut subset Hindgut Head Fat body Ectoderm Polarized Ectoderm CNS Ventral nerve cord Blastoderm nuclei Atrium Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around ectoderm nuclei Foci Small Foci Small Foci in tracheal cells Small foci in Proventriculus Small Foci in PNS Small Foci in hindgut Small Foci in head Small Foci in ectoderm Small Foci in CNS Cell junction associated |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing discs particular pattern Wing disc few cells - dots Expressed in eye disc Eye disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Ventral nerve cord - allover Localized to axons Expressed in brain lobes Brain lobes allover |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' Lymph Glands Expressed in lymph glands Lymph glands all over |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern PNS Head Ectoderm Dorsal vessel CNS Ventral nerve cord Polarized VNC Axons Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around dorsal vessel nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in CNS Accumulation nuclear subregion Foci Small Foci Small Foci in PNS Small Foci in dorsal vessel Small Foci in CNS Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel Reannotated. |
| Leading edge, ectoderm. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Basal localization Basal enrichment Apical localization Apical enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Medium expression (21-40ms exp) Localized Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic PNS Neuroblasts Hindgut CNS Ventral nerve cord Perinuclear VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe, nice insitu... |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole plasm Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Posterior localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Cell junction associated Basal localization Basal enrichment Apical localization Apical enrichment Anterior localization Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cell junction associated Apical localization Apical enrichment Diffuse apical localization Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in neuroblasts Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Other subcellular pattern Cytoplasmic foci Germ cell localization Germ cell enrichment Embryonic localization patterns Ventral localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern Salivary glands Posterior spiracles PNS Neuroblasts Polarized Neuroblasts Midgut Midgut subset Head CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around midgut nuclei Perinuclear around CNS nuclei Perinuclear 'rings' Foci Small Foci Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Perinuclear localization Half moon perinuclear Expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Expressed in midgut Subcellular localization in midgut Midgut - Perinuclear Midgut - foci Expressed in gastric caeca Nascent transcript in gastric caeca Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs few cells - dots Leg discs - outside Expressed in eye disc Eye disc basal Eye disc - center round cells Expressed in antennal disc Antennal disc - localized outside Antennal disc - center round cells |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Perinuclear Salivary gland - Half moon perinuclear Nascent transcript in salivary gland Expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Perinuclear Ring gland - Cytoplasmic foci Nascent transcript in ring gland Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel Goes from all-over to 'posterior enriched'. |
| Anterior zygotic and posterior maternal expression. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Basal exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands PNS Midgut Hindgut Head Ectoderm CNS Ventral nerve cord Perinuclear VNC Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Nascent transcript in hindgut Hindgut allover Hindgut - Midgut border |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc photo-receptors Eye disc particular pattern Expressed in antennal disc Antennal disc - center round cells |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area Localized to axons Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - nuclear dots Salivary gland - membrane Salivary gland - Cytoplasmic foci Salivary gland - Small foci Nascent transcript in salivary gland Expressed in salivary glands Salivary glands allover |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Lymph Glands Expressed in lymph glands Lymph glands all over |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel |
| Not enough larval fat body images. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole plasm Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Cytoplasmic foci Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation nuclear subregion Cytoplasmic foci Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands PNS CNS Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Foci Small Foci Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel Pole plasm enriched, very strong expression (3ms photos). Mild gradient Posterior to Anterior. |
| Posterior to anterior gradient. |
| Very specific and dynamic expression. Perinuclear. Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei All blastoderm nuclei Persistent widespread Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Localized Subcellular localization patterns Exclusionary patterns Apical localization Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in mesoderm Expression in ectoderm Expression in API |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen PNS Head CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Foci Small Foci Small Foci in PNS Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus - Few foci Expressed in midgut Subcellular localization in midgut Midgut - Nuclear Midgut - Subnuclear Midgut - foci Nascent transcript in midgut Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Few gastric caeca foci |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Expressed in leg discs Leg discs few cells - dots Expressed in eye disc Morphogenetic furrow High expression in broken eye disc Eye disc photo-receptors Eye disc few cells - dots Expressed in antennal disc Antennal disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area Ventral nerve cord - nuclear localization Ventral nerve cord - foci or 'dots' Ventral nerve cord - allover Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Perinuclear Salivary gland - Half moon perinuclear Nascent transcript in salivary gland Expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Foci in larval muscles edge Muscles allover - Larva Expressed in heart |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Nascent transcript in ring gland Expressed in ring gland Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe. We use it as one positive control for the screen. |
| Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in ectoderm Ubiquitous Localized Subcellular localization patterns Perinuclear localization Perinuclear around all nuclei Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Neuroblasts CNS Ventral nerve cord Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH (3 probe sets) |
| -isoform corn-RC transcript was targeted |
| -weak expression in the late embryos that appear as small points along the ectoderm of the each segment (trunk and abdominal) located dorsolateral. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Foci Small Foci Small Foci in PNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Hindgut allover Hindgut - Midgut border Expressed in gastric caeca Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs particular pattern |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Expressed in salivary glands Salivary glands allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Posterior spiracles PNS Pharynx Muscles-embryo Muscles - subregion Fat body CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Foci Small Foci Small Foci in Pharynx Small Foci in muscle cells Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Cytoplasmic foci Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment Pole cell localization Pole cell enrichment Embryonic localization patterns Dorsal localization Degraded Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Expression in neuroblasts Expression in ectoderm Expression in amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Germ cell localization Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic PNS CNS Ventral nerve cord Perinuclear VNC Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Foci Small Foci Small Foci in PNS Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -detected using HCR-FISH protocol (3 probe sets) |
| -probes were designed against dap-RA isoform transcript |
| -really neat expression patterns |
| -images are from two separate experiments under identical conditions for better coverage |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Apical localization Apical enrichment Posterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS CNS Ventral nerve cord Perinuclear VNC Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around CNS nuclei Foci Small Foci Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous Borderline between 'not expressed' and 'low expression' |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc particular pattern |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - perinuclear localization Ventral nerve cord - foci or 'dots' Expressed in brain lobes Brain lobes allover Brain lobes - Significant area Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Pole cell localization Cytoplasmic foci Apical localization Apical enrichment Discrete apical foci Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in ectoderm Expression in API Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Sensory system Segmented pattern Salivary glands PNS Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Ectoderm CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in tracheal nuceli Nuclear in ectoderm Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus Foci Small Foci Small Foci in PNS Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Nuclear localization and other patterns. |
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Sensory system Salivary glands Salivary glands lumen PNS Head Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Accumulation nuclear subregion Accumulation entire nucleus Foci Small Foci Small Foci in other tissues Small Foci in amnioserosa |
Larva
| Area | Images | Terms |
|---|---|---|
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc particular pattern Eye disc - center round cells |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area Brain lobes - Foci or dots |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Anterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation in subset nuclei Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Dorsal blastoderm nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation nuclear subregion Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Perinuclear around yolk nuclei Sensory system PNS Head CNS Ventral nerve cord Perinuclear VNC Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Foci Small Foci Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Comments
| Re-annotated - more terms added. |
| Need more images to get the full story. In situ done in 2005-2006, before we started screening late stage embryos. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Single stripe Central band of nuclei Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Basal localization Basal enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Anterior blastoderm nuclei Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic PNS Germ cell nuclei Ectoderm CNS Ventral nerve cord Polarized VNC Perinuclear VNC Nuclear - VNC Axons Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus Foci Bigger Foci Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Single stripe Expression in ectoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Degraded Degraded partially stage 7 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Single stripe Expression in ectoderm Anterior blastoderm nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands lumen PNS Head Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Axons Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in CNS Foci Small Foci Small Foci in PNS Small Foci in head Small Foci in CNS Small Foci in Brain Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Not every embryo has a pole cell excluded pattern. |
| Head, specific. |
| Good probe on gel. Small, localized foci. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Exclusionary patterns Yolk cortex exclusion Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands Salivary glands lumen Posterior spiracles PNS CNS Ventral nerve cord Polarized VNC Perinuclear VNC Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around CNS nuclei |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
Adult
| Area | Images | Terms |
|---|
Comments
| Nice nervous system. |
| Good probe on gel. Small foci. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Cell junction associated Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Exclusionary patterns Cell junction associated Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Localized Subcellular localization patterns Cell junction associated Apical localization Apical enrichment Diffuse apical localization Apical Localization Neuroblasts Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic PNS Neuroblasts Polarized Neuroblasts CNS Ventral nerve cord Polarized VNC Perinuclear VNC Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around CNS nuclei |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Proventriculus allover Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Hindgut Axons Expressed in gastric caeca Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs few cells - dots Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc few cells - dots Eye disc basal Expressed in antennal disc Antennal disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands Borderline between 'not expressed' and 'low expression' |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Nascent transcript in ring gland Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands Lymph glands all over |
Adult
| Area | Images | Terms |
|---|
Comments
| Nice apical localizatin in neuroblasts during and after delamination. |
| Zygotically expressed RNA localizes apically and along the lateral membrane. RNA is likely cell junction associated. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Anterior blastoderm nuclei Maternal Localized Subcellular localization patterns Exclusionary patterns Yolk cortex exclusion Pole cell exclusion Apical exclusion Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Anterior blastoderm nuclei Localized Subcellular localization patterns Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Sensory system Segmented pattern PNS Midgut CNS Ventral nerve cord Axons Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Foci Small Foci Small Foci in PNS Small Foci in midgut Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Gap-gene pattern Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Cytoplasmic foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Gap-gene pattern Expression in mesoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Ventral localization Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Expression in neuroblasts Expression in mesoderm Expression in ectoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern PNS CNS Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Head Ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RC transcript isoform |
| -neat PNS pattern; known to co-localize with ppk (CG3478) expression patterns |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Segment polarity pattern Pair-rule pattern Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Ventral localization Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Dorsal localization Cytoplasmic foci Apical localization Apical enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Posterior blastoderm nuclei Expression in ectoderm Dorsal blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Apical localization Apical enrichment Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Degraded Degraded partially stage 7 |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Dorsal blastoderm nuclei Degraded Degraded partially stage 8 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Maxillary complex Head Head epidermis Clypeo-labral bud Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -retested recently for late stage coverage (2025); appears to be expressed in a different program in the gnathal elements and sensory regions in late stages |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Random blastoderm nuclei Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Random blastoderm nuclei Localized Subcellular localization patterns Exclusionary patterns Cytoplasmic foci Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern PNS Neuroblasts CNS Ventral nerve cord Foci - VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around CNS nuclei Other subcellular pattern Foci Small Foci Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Small foci. |
| Small foci. |
| Re-annotated |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands PNS CNS Ventral nerve cord Foci - VNC Axons Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in CNS Accumulation nuclear subregion Foci Small Foci Small Foci in CNS Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Salivary glands and tracheal lumen, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Pole cell exclusion Cell division apparatus Chromatin associated metaphase Apical localization Apical enrichment Diffuse apical localization Local terms for zygotic transcripts Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Strong expression (1-20ms exp) Localized Subcellular localization patterns Exclusionary patterns Apical localization Apical enrichment Apical clusters Local terms for zygotic transcripts Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Strong expression (1-20ms exp) Localized Subcellular localization patterns Apical localization Apical enrichment Apical Localization Neuroblasts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern PNS CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus - Few foci Proventriculus allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover Expressed in esophagus |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Expressed in eye disc Morphogenetic furrow Eye disc few cells - dots Expressed in antennal disc Antennal disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Cytoplasmic foci Salivary gland - Small foci Expressed in salivary glands Salivary glands allover |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands Lymph glands all over |
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Localized Subcellular localization patterns Ventral localization Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Degraded Degraded partially stage 5 Degraded partially stage 4 |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Localized Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts Localized Embryonic localization patterns Ventral localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Head CNS Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| ovary and testis |
|
3rd Instar Larval Tissues Larval Ovary and Testis-Larva Not expressed in larval testis |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Interesting expression and localization patterns. |