For a full list of searchable terms, please see the term page.
NOTE: It takes a few seconds for the term suggestions to load.
Result Summary
| Number of genes | 47 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Cell division apparatus Other cell division pattern Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Cytoplasmic foci Cell division apparatus Other cell division pattern Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Posterior spiracles Specific posterior spiracle pattern Pharynx Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in Pharynx Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Very low probe on gel. |
| Very interesting patterns. |
| Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Posterior blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Yolk cortex localization Yolk cortex enrichment Posterior localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Dorsal localization Cell division apparatus Chromatin associated metaphase Basal localization Basal enrichment Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Dorsal blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Yolk plasm localization Yolk plasm enrichment Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Posterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Maternal Localized Yolk plasm localization Yolk plasm enrichment Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Perinuclear around yolk nuclei Inside Yolk nuclei Salivary glands Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Nuclei-associated localization Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Salivary glands, probably non-specific. |
| The probe looks good on gel The photos are from a combination of 2 experiments. One had a brighter probe than the other, which explains why some photos are brighter than others. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization Other subcellular pattern Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Cell junction associated Basal localization Basal enrichment Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 7 Degradation zygotic transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| I see sub-nuclear localization. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei All blastoderm nuclei Transiently widespread Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Cell division apparatus Spindle midzone localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion Degraded Degraded completely stage 7 Degradation maternal transcripts |
| stage 8-9 |
Stage 8-9 Non-Expressed |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Re-annotated. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Posterior localization RNA islands Pole plasm Pole buds Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei All yolk nuclei Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around all nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Apical exclusion Local terms for zygotic transcripts Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Maternal Localized Subcellular localization patterns Nuclei-associated localization Posterior localization Pole cell localization Pole cell enrichment Local terms for zygotic transcripts Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in API Anterior blastoderm nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Maternal mRNA is posteriorly localized in pole plasm and forms RNA islands around pole cell nuclei. Localization persists in pole/germ cells until 6-7. |
| Zygotic mRNA expressed from yolk nuclei accumulates in clouds surrounding the yolk nuclei. The RNA also seems to accumulate in or tightly around a few nuclei that are entering the blastoderm layer from the yolk. |
| -Missing late stages - needs to be retested for full coverage of expression patterns (2024) |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 9 Degradation maternal transcripts |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Some dots in some nuclei |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Transiently widespread Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation in subset nuclei Membrane associated Exclusionary patterns Pole cell exclusion Cell division apparatus Spindle midzone localization Apical localization Apical enrichment Diffuse apical localization Local terms for zygotic transcripts Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Localized Subcellular localization patterns Membrane associated Apical localization Apical enrichment Diffuse apical localization |
| stage 8-9 |
|
Stage 8-9 Expressed |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Zygotic RNA exhibits dynamic localization patterns. It seems to accumulate in or tightly around a subset of blastoderm nuclei. Shows spindle midzone localization during nuclear division. Also accumulates apically and seems to be membrane associated both apically and laterally. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei All cortical nuclei Ubiquitous Localized Subcellular localization patterns Nuclei associated localization Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Yolk plasm localization Ventral localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Dorsal localization Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in ectoderm Expression in API All blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in ectoderm Expression in API All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Germ cell localization Germ cell enrichment Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Non-Expressed |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -probes were designed against RA isoform |
| -tested using HCR-FISH probes (3 probe sets) |
| -expression pattern aligns well with modENCODE RNA-seq data -observed possible early lateral or dorsal-ventral localization of early ectoderm |
| -bright foci is mostly background |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Dorsal yolk nuclei Germ cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Germ cell localization Germ cell enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Salivary glands PNS Pharynx Hemocytes Germ cell nuclei Gonads Ectoderm CNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Perinuclear around hemocytes Nuclei-associated localization Nuclear in PNS Nuclear in hemocytes Nuclear in ectoderm Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| The probe looks good on gel. |
| Dynamic expression. Salivary glands, probably non-specific |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Dorsal blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Dorsal localization Cytoplasmic foci Apical localization Apical enrichment Apical clusters Degraded Degraded partially stage 5 |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Expression in ectoderm Dorsal blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts Expression in mesoderm Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands Head Blastoderm nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe on gel Nice in situ! |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Basal localization Basal enrichment Local terms for zygotic transcripts Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Basal localization Basal enrichment Apical localization Apical enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Hard to see expression with the strong DAPI...(Ronit) |
| Zygotic RNA seems to accumulate transiently in some of the posterior blastoderm nuclei. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Cell junction associated Cell division apparatus Other cell division pattern Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Ubiquitous Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Degraded Degraded partially stage 8 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Salivary glands Salivary glands - specific region Blastoderm nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Very low probe on gel... nice data (you never know) |
| Nice! |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei All blastoderm nuclei Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Cell division apparatus Other cell division pattern Basal localization Basal enrichment Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in all nuclei Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in ectoderm Expression in amnioserosa Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Ectoderm CNS Ventral nerve cord Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Nuclear in CNS Nuclear in amnioserosa |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Midgut allover Expressed in hindgut Hindgut allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Cytoplasmic foci Expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Distal tip region-TE Not Expressed in distal tip region -TE Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Individualizing Spermatids - TE Waste Bag - TE Cytoplasmic in individualizing spermatids - TE Cyst cells -TE Tail cyst cell - TE Retracting/Coiling Tail Cyst Cell - TE Retracting/Coiling Tail Cyst Cell Cytoplasm - TE Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed High - TE Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Expression level-SV Expressed High-SV Cell type - SV Cytoplasmic-SV Membrane enriched - SV Cytoplasmic Foci - SV Ejaculatory Duct Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Cytoplasmic foci-SC Main cells -AG Cytoplasmic-MC |
Comments
| All nuclei. Strong in the Amnioserosa. |
| Enriched in the amnioserosa, the central nervous system, the ventral midline, and maybe the tracheal pits. |
| Anterior to posterior gradient Subnuclear localization. |
| The probe is low, but good. Nice insitu data!!! |
Probe CR43264-RA Links: Permalink
Genes: CR43264
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Yolk plasm localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Medium expression (21-40ms exp) Localized Yolk plasm localization Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Pole cell localization Pole cell enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Medium expression (21-40ms exp) Localized Yolk plasm localization Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Cytoplasmic foci Germ cell localization Germ cell enrichment Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Perinuclear around yolk nuclei Germ cell nuclei Gonads Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Foci Small Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 2 probe sets targeting the RA isoform transcript |
| -this non-coding gene is an extremely highly expressed lncRNA in male testes according to scRNA-seq data, and exhibits a strong nuclear pattern in in embryo yolk, pole cell, and random blastoderm cells |
Probe CR43314-RA Links: Permalink
Genes: CR43314
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Central band of nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Pole cell exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Anterior blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Expression in API Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Posterior spiracles Specific posterior spiracle pattern PNS Midgut Midgut subset Head Head epidermis Clypeo-labral bud Anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in midgut nuclei Nuclear in ectoderm Foci Small Foci Small Foci in PNS Small Foci in ectoderm Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
| -images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
Probe CR46003-RC Links: Permalink
Genes: CR46003
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Localized Subcellular localization patterns Ventral localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Dorsal localization Cytoplasmic foci Basal localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Dorsal blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Degraded Degraded partially stage 7 |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Cytoplasmic foci Embryonic localization patterns Ventral localization Degraded Degraded partially stage 8 Degraded completely stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Neuroblasts Polarized Neuroblasts Head Head epidermis Ectoderm Polarized Ectoderm CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Polarized CNS Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in CNS Foci Small Foci Small Foci in CNS Small Foci in Brain Basal enrichment Basal (outside) in CNS Basal (outside) in brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RC isoform transcript |
| -the gene is a lncRNA with quite interesting patterns in the CNS (brain and VNC) |
| -seems to be expressed in the anterior transverse furrow, shown in stage 6-7 images |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Yolk cortex exclusion Pole cell exclusion Apical exclusion Local terms for zygotic transcripts Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Localized Subcellular localization patterns Exclusionary patterns Basal exclusion Apical exclusion Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Non-Expressed Degraded Degraded completely stage 8 Degradation zygotic transcripts Degradation maternal transcripts |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Local terms for zygotic transcripts Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Local terms for zygotic transcripts Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Nuclei-associated localization Local terms for zygotic transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Sensory system PNS Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Ventral localization Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands Germ cell nuclei Gonads Brain Blastoderm nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Very low probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Posterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Posterior blastoderm nuclei Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Local terms for zygotic transcripts Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Degraded Degraded partially stage 7 Degradation zygotic transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Nuclei-associated localization |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Re-annotated with few changes. |
| Maternal RNA excluded from the apical cytoplasm and pole cells, and gets degraded by stage 5 Zygotic RNA expressed from blastoderm and yolk nuclei Zygotic RNA seems to accumulate transiently in expressing blastoderm nuclei. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Transiently widespread Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in API Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in API Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Tracheal system Tracheal pits Pharynx Head Ectoderm Blastoderm nuclei Atrium Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in tracheal nuceli Nuclear in pharynx Nuclear in other cells Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| In many nuclei there appear to be several nuclear foci in addition to sites of nascent transcript synthesis. |
| RNA seems to accumulate around most yolk nuclei and expressing blastoderm nuclei. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Expression in API Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Apical exclusion Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Muscles-embryo Midgut Midgut subset Malpighian tubules CNS Ventral nerve cord Nuclear - VNC Foci - VNC Brain Localized Subcellular localization patterns Nuclei-associated localization Nuclear in hindgut nuclei Nuclear in CNS Foci Small Foci Small Foci in PNS Small Foci in midgut Small Foci in Malpighian tubules Small Foci in CNS Basal enrichment Basal in midgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -strong posterior midgut primordium expression |
| -possible expression in sensory organs in head and posterior epidermis |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around all nuclei Degraded Degraded partially stage 3 Degraded partially stage 2 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Apical clusters Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Exclusionary patterns Apical localization Apical enrichment Apical clusters Embryonic localization patterns Exclusionary patterns Pole cell exclusion Degraded Degraded completely stage 7 Degradation zygotic transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Ubiquitous Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Degraded |
| stage 10-17 |
Stage 10-17 Non-Expressed |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -not detected during early stages of germ band elongation (8-9); possibly weakly ubiquitous at these later stages |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Other subcellular pattern Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Apical clusters Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Dorsal blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Membrane associated Exclusionary patterns Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Membrane associated Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Ectoderm CNS Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Membrane associated Foci Small Foci Small Foci in ectoderm Small Foci in CNS Small Foci in Brain Cell junction associated Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Wing disc few cells - dots Expressed in leg discs Leg discs few cells - dots Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc few cells - dots Expressed in antennal disc Antennal disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. Interesting patterns. |
| Apical localization. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Single stripe Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Single stripe Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Basal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Single stripe Segmented pattern Expression in mesoderm Expression in ectoderm Expression in amnioserosa Dorsal blastoderm nuclei Medium expression (21-40ms exp) Localized Embryonic localization patterns Posterior localization Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Muscles - subregion Hindgut Ectoderm CNS Ventral nerve cord Strong expression (1-20ms exp) Localized Subcellular localization patterns Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -possesses a striking segmented pattern in the epidermis, mesoderm, and VNC; reports of expression in the cardiac primordium and dorsal vessel were not clearly observed and could possibly be amnioserosa expression |
| -a classic homeobox gene |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Pole cell nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Germ cell nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Blastoderm nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Yolk cortex exclusion |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Apical exclusion Local terms for zygotic transcripts Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Accumulation entire nucleus Local terms for zygotic transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Accumulation entire nucleus Local terms for zygotic transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Head CNS Ventral nerve cord Nuclear - VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in CNS Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Borderline between 'not expressed' and 'low expression' |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Not expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Re-annotated RW |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cell division apparatus Chromatin associated metaphase Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Central band of nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic CNS Brain Blastoderm nuclei Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Zygotic expressioin occurs early on in stage 4, RNA appears to accumulate in clusters around metaphase chromosomes at stage 4. |
| Zygotic expression occurs broadly in blastoderm nuclei, except for the most anterior and posteriro nuclei. |
| Possibly weak pole plasm enrichment, although too weak to document for sure. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Random cortical nuclei Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Exclusionary patterns Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation entire nucleus |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic CNS Ventral nerve cord Brain Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good proe on gel. |
| Anterior excluded, YN excluded. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Weak expression (>41ms exp) Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Foci Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. Nice in situ data. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Anterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation in subset nuclei Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Dorsal blastoderm nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation nuclear subregion Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Perinuclear around yolk nuclei Sensory system PNS Head CNS Ventral nerve cord Perinuclear VNC Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Foci Small Foci Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Comments
| Re-annotated - more terms added. |
| Need more images to get the full story. In situ done in 2005-2006, before we started screening late stage embryos. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Segment polarity pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Expression in API Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in mesoderm Expression in ectoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Proventriculus Pharynx Hindgut Specific hindgut area or cells Head Head epidermis Esophagus Ectoderm Clypeo-labral bud Localized Subcellular localization patterns Nuclei-associated localization Nuclear in proventriculus Nuclear in pharynx Nuclear in ectoderm Foci Small Foci Small foci in Proventriculus Small Foci in Pharynx Small Foci in hindgut Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei All blastoderm nuclei Persistent widespread Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation in subset nuclei Local terms for zygotic transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Local terms for zygotic transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Nuclei-associated localization Local terms for zygotic transcripts |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Perinuclear around all nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Cell division apparatus Chromatin associated metaphase Local terms for zygotic transcripts Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Exclusionary patterns Local terms for zygotic transcripts Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Central yolk nuclei. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Right flanking nuclei Left flanking nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Right flanking nuclei Left flanking nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Segment polarity pattern Expression in neuroblasts Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Perinuclear around yolk nuclei Tracheal system Tracheal pits Segmented pattern Neuroblasts CNS Ventral nerve cord Polarized CNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Segment polarity pattern Pair-rule pattern Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Ventral localization Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Dorsal localization Cytoplasmic foci Apical localization Apical enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Posterior blastoderm nuclei Expression in ectoderm Dorsal blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Apical localization Apical enrichment Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Degraded Degraded partially stage 7 |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Dorsal blastoderm nuclei Degraded Degraded partially stage 8 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Maxillary complex Head Head epidermis Clypeo-labral bud Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -retested recently for late stage coverage (2025); appears to be expressed in a different program in the gnathal elements and sensory regions in late stages |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Basal localization Apical localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in mesoderm Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Ventral localization Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Single stripe Expression in ectoderm Anterior blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Sensory system Posterior spiracles Muscles-embryo Visceral mesoderm Hindgut Head Head epidermis Dorsal vessel CNS Ventral nerve cord Foci - VNC Axons Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Foci Small Foci Small Foci in PNS Small Foci in head Small Foci in ectoderm Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -neat and diverse expression patterns throughout development |
| -images are from two separate experiments under identical conditions for better coverage |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei All cortical nuclei Strong expression (1-20ms exp) |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Local terms for zygotic transcripts Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Local terms for zygotic transcripts Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Local terms for zygotic transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Head CNS Ventral nerve cord Nuclear - VNC Brain Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in other cells Nuclear in ectoderm Nuclear in CNS Nuclear in amnioserosa Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus perinuclear Proventriculus perinuclear - few cells Expressed in midgut Subcellular localization in midgut Midgut - Nuclear Midgut - Subnuclear Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - Nuclear in Malpighian tubules - Subnuclear in Expressed in hindgut Subcellular localization in hindgut Nuclear in hindgut Hindgut - subnuclear localization Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca - nuclear localization Gastric caeca - subnuclear localization Nascent transcript in gastric caeca Expressed in esophagus |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - nuclear localization Ventral nerve cord - subnuclear localization Expressed in brain lobes Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - nuclear dots Nascent transcript in salivary gland |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Specific testis expression localization Larval testis foci |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva |
| tracheal system |
|
3rd Instar Larval Tissues Tracheal System Expressed in tracheal tubes |
| lymph glands and ring glands |
|
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Testicular duct-TE Nuclear in Testicular Duct - TE Terminal Epithelium - TE Nuclear in terminal epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatogonium-TE Nuclear in spermatogonium-TE Spermatocytes -TE Nuclear in Spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Individualizing Spermatids - TE Waste Bag - TE Cytoplasmic in individualizing spermatids - TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Pigment cells -TE Nuclear in pigment cells -TE Muscle cells - TE Nuclear in muscle cells -TE Cytoplasmic in muscle cells - TE Cyst cells -TE Tail cyst cell - TE Individualizing Tail Cyst Cell - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Nuclear Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Germline stem cell Cytoplasmic in germline stem cells - TE Early cyst cell Cytoplasmic in early cyst cell Clusters in early cyst cell-TE Cyst stem cell Cytoplasmic in cyst stem cell Lower expression in tip region Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Expression level-SV Expressed Medium - SV Cell type - SV Nuclear-SV Cytoplasmic-SV Perinuclear - SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Expression level-ED Expressed Medium - ED Cell type - ED Nuclear-ED Cytoplasmic-ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Diffuse foci-AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Nuclear-SC Nuclear Foci-SC Cytoplasmic-SC Multivesicular bodies - SC Main cells -AG Nuclear- MC Nuclear foci-mc Cytoplasmic-MC Cytoplasmic Foci - AG |
Comments
| Nuclear |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Yolk nuclei Subset yolk nuclei Random yolk nuclei Ubiquitous Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Basal localization Basal enrichment Degraded Degraded partially stage 4 Degradation zygotic transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Posterior blastoderm nuclei Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Expression in neuroblasts Expression in ectoderm Expression in API Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern PNS Optic lobes primordium Neuroblasts Polarized Neuroblasts Midgut Maxillary complex Head Head epidermis CNS Ventral nerve cord Polarized VNC Perinuclear VNC Foci - VNC |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -expression seen in the stomatogastric sensory primordium, and the anterior and posterior midgut primordium; forms a complex along with ac (CG3796) so should colocalize in neuronal regions |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Random cortical nuclei |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Yolk plasm localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation in subset nuclei Local terms for zygotic transcripts |
| stage 6-7 |
|
Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation zygotic transcripts |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Re-annotated. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Degraded Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Neuroblasts Foregut CNS Ventral nerve cord Foci - VNC Brain Localized Subcellular localization patterns Foci Small Foci Small Foci in foregut Small Foci in ectoderm Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
| -good marker for the early neuroectoderm |
| -RNAseq data and experiments done by BDGP suggest no maternal expression, however we observe what appears to be maternal expression that borders between expressed and not expressed |
Probe CR34590 Links: Permalink
Genes: snoRNA:(psi)18S-531
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Accumulation in all nuclei Cytoplasmic foci Posterior localization Pole cell localization Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Salivary glands Strong expression (1-20ms exp) Localized Subcellular localization patterns |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Probe CR33754 Links: Permalink
Genes: snoRNA:(psi)28S-291
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Yolk cortex localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in all nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Midgut Hindgut Foregut Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Foci Small Foci Small Foci in salivary glands Small Foci in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Yolk nuclei Subset yolk nuclei Random yolk nuclei Cortical nuclei All cortical nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus Other cell division pattern |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Gap-gene pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Basal exclusion Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Terminal-gene pattern |
| stage 8-9 |
|
Stage 8-9 Non-Expressed Degraded Degraded completely stage 8 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. Partly nascent, partly nuclear subregion |
| Salivary glands and tracheal lumen, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Degraded Degraded partially stage 2 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Membrane associated Exclusionary patterns Pole cell exclusion Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Local terms for zygotic transcripts Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Strong expression (1-20ms exp) |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Strong expression (1-20ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Segmented pattern Ectoderm Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Comments
| Need more images to get the full story. In situ done before we started screening late stage embryos. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Ectoderm CNS Ventral nerve cord Foci - VNC Brain Localized Subcellular localization patterns Foci Small Foci Small Foci in ectoderm Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -good marker for the neuroectoderm region of the presumptive ventral nerve cord on the germ band; possible weak but faint epidermal signal detected in later embryos |