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Result Summary
| Number of genes | 49 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Malpighian tubules Head Head epidermis Ectoderm Anal pad |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Other subcellular pattern Membrane associated Exclusionary patterns Pole cell exclusion Basal exclusion Dorsal localization Cytoplasmic foci Cell junction associated Apical localization Apical enrichment Discrete apical foci Apical clusters Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Other subcellular pattern Membrane associated Exclusionary patterns Yolk plasm exclusion Basal exclusion Cytoplasmic foci Cell junction associated Apical localization Apical enrichment Discrete apical foci Apical clusters Embryonic localization patterns Exclusionary patterns Pole cell exclusion Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Other subcellular pattern Membrane associated Cytoplasmic foci Apical localization Apical enrichment Discrete apical foci Apical clusters Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Posterior spiracles Head Head epidermis Esophagus Ectoderm Polarized Ectoderm CNS Polarized CNS Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in CNS Accumulation nuclear subregion Foci Small Foci Small Foci in ectoderm Small Foci in amnioserosa Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Individualizing Spermatids - TE Cystic bulge - TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Distal tip region-TE Not Expressed in distal tip region -TE Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Cell type - SV Cytoplasmic-SV Perinuclear - SV Membrane enriched - SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Membrane enriched - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Low - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC Main cells -AG Cytoplasmic-MC Membrane Enriched-MC |
Comments
| Good probe on gel, a bit low. Very nice insitu... interesting patterns. |
| Membrane localization apically, in small clusters. Very interesting. |
| Membrane localization apically, in clusters. Very interesting. |
| Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Head Head epidermis Ectoderm Localized Subcellular localization patterns Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -probes were designed against RA isoform |
| -tested using HCR-FISH probes (3 probe sets) |
| -epidermal striping pattern between segments observed in late stages; possibly related to sensory hairs and denticle belts |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk cortex localization Yolk cortex enrichment Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Ubiquitous Maternal Localized Yolk plasm localization Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Basal localization Basal enrichment Apical localization Apical enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in API Expression in amnioserosa All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 8 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Segmented pattern Pharynx Neuroblasts Midgut Head Head epidermis Ectoderm Polarized Ectoderm CNS Ventral nerve cord Clypeo-labral bud Brain Atrium Anal pad Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -appears ubiquitously throughout development with some mesoectodermal enrichment during the germ band elongation phases (appears as stripes) |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion |
|
| stage 6-7 |
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
|
| stage 8-9 |
Stage 8-9 Non-Expressed Degraded Degraded completely stage 8 |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Salivary glands Posterior spiracles Specific posterior spiracle pattern Head Head epidermis Ectoderm Polarized Ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Nuclei-associated localization Nuclear in ectoderm Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in hindgut Subcellular localization in hindgut Hindgut foci Hindgut allover |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Nice Embryo data |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Head Head epidermis Ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Expression level-TE Expressed Low - TE Testis Cell type and stage - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Spermatocytes -TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Distal tip region-TE Expressed in distal tip region - TE Seminal Vesicle Subcellularly localized - SV Lumenal-SV Non-particulate - SV Ejaculatory Duct Expression level-ED Expressed Low - ED Subcellularly localized - ED Lumenal-ED Anterior ejaculatory duct papilla-ED Entry ring-ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Low - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC |
Comments
| Salivary glands, probably non-specific. |
| Good probe on gel, a bit low. |
| Interesting localization in adult ejaculatory duct 'ring'. |
Probe CG16815-RB Links: Permalink
Genes: CG16815
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Single stripe Posterior blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Posterior localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Exclusionary patterns Yolk cortex exclusion Pole cell exclusion Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Degraded Degraded partially stage 7 Degraded partially stage 6 |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Degraded Degraded partially stage 8 Degraded completely stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Head Head epidermis Ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
Probe CG31195-RB Links: Permalink
Genes: CG31195
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Hindgut Head Head epidermis Ectoderm CNS Ventral nerve cord Axons Anal pad |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern Salivary glands Salivary glands lumen Salivary glands - specific region Proventriculus Posterior spiracles Pharynx Midgut Hindgut Head Head epidermis Esophagus Ectoderm Blastoderm nuclei Anal pad Localized Subcellular localization patterns Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Small foci. |
| small foci. |
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Head Head epidermis Ectoderm Polarized Ectoderm Atrium Localized Subcellular localization patterns Apical enrichment Apical in other tissues |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus - Few foci Particular proventriculus area |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - Perinuclear Salivary gland - nuclear dots Salivary gland - Cytoplasmic foci Expressed in salivary glands Salivary glands allover |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe on gel. |
| Interesting anterior cells on late embryos. |
| Interesting brain lobe expression in larval brains. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Head Head epidermis Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Membrane associated |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. Borderline between 'not expressed' and 'low expression' |
| Head epidermis - membrane looking pattern. Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands Posterior spiracles Specific posterior spiracle pattern PNS Head Head epidermis Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Not expressed in larval muscles - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Not expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe on gel. |
| Interesting anterior late embryo expression and sensory neurones. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Apical exclusion Cytoplasmic foci Basal localization Basal enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Segmented pattern Proventriculus Hindgut Head Head epidermis Foregut Esophagus Ectoderm Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small foci in Proventriculus Small Foci in hindgut Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - Nuclear Midgut - Subnuclear Midgut - foci Specific midgut cells Nascent transcript in midgut Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Hindgut allover Hindgut - Midgut border Expressed in gastric caeca Subcellular localization in gastric caeca Perinuclear gastric caeca Half moon perinuclear gastric caeca Gastric caeca - nuclear localization Gastric caeca - subnuclear localization Gastric caeca allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Link added manually |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Pole cell localization Pole cell enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in neuroblasts Expression in mesoderm Anterior blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Cytoplasmic foci Cell junction associated Germ cell localization Germ cell enrichment |
| stage 10-17 |
Stage 10-17 Expressed Zygotic Segmented pattern Neuroblasts Head Head epidermis Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Need more images to get the full story. In situ done in 2006, before we started screening late stage embryos. |
| Re-annotated |
Probe CR43314-RA Links: Permalink
Genes: CR43314
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Central band of nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Pole cell exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Anterior blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Expression in API Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Posterior spiracles Specific posterior spiracle pattern PNS Midgut Midgut subset Head Head epidermis Clypeo-labral bud Anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in midgut nuclei Nuclear in ectoderm Foci Small Foci Small Foci in PNS Small Foci in ectoderm Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
| -images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
Probe CR46003-RC Links: Permalink
Genes: CR46003
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Localized Subcellular localization patterns Ventral localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Dorsal localization Cytoplasmic foci Basal localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Dorsal blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Degraded Degraded partially stage 7 |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Cytoplasmic foci Embryonic localization patterns Ventral localization Degraded Degraded partially stage 8 Degraded completely stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Neuroblasts Polarized Neuroblasts Head Head epidermis Ectoderm Polarized Ectoderm CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Polarized CNS Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in CNS Foci Small Foci Small Foci in CNS Small Foci in Brain Basal enrichment Basal (outside) in CNS Basal (outside) in brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RC isoform transcript |
| -the gene is a lncRNA with quite interesting patterns in the CNS (brain and VNC) |
| -seems to be expressed in the anterior transverse furrow, shown in stage 6-7 images |
Probe GM08252 Links: Permalink
Genes: flam flam-intron
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 1 Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Pronucleus-associated Perinuclear localization Perinuclear around all nuclei Intranuclear accumulation Accumulation in all nuclei Cell division apparatus Chromatin associated metaphase Local terms for zygotic transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei All yolk nuclei Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci Cell division apparatus Chromatin associated metaphase Local terms for zygotic transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus Chromatin associated metaphase Local terms for zygotic transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Apical localization Local terms for zygotic transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Tracheal system Specific tracheal branches Segmented pattern Salivary glands Posterior spiracles Specific posterior spiracle pattern Midgut Head Head epidermis Ectoderm Atrium Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body Expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Proventriculus allover Particular proventriculus area Expressed in midgut Subcellular localization in midgut Midgut - foci Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - Nuclear in Malpighian tubules - Subnuclear in Nascent transcript in malpighian tubules Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Nuclear in hindgut Hindgut - subnuclear localization Hindgut foci Nascent transcript in hindgut Hindgut allover Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca - nuclear localization Gastric caeca - subnuclear localization Nascent transcript in gastric caeca Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs particular pattern Leg discs few cells - dots Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc few cells - dots Eye disc - center round cells Expressed in antennal disc |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - nuclear dots Salivary gland - Cytoplasmic foci Cytoplasmic foci in Salivary Gland Lumen Salivary gland - apical Salivary gland - lumen Nascent transcript in salivary gland Expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Nuclear Ring gland - Subnuclear Nascent transcript in ring gland Expressed in ring gland |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatocytes -TE Y-Loop pattern - TE Nuclear in Spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Nuclear in round spermatids-TE Cytoplasmic in round spermatids-TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Sperm Tail Decoration-TCC associated Retracting/Coiling sperm tail-TCC associated Retracting/Coiling Tail Cyst Cell - TE Retracting/Coiling Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Nuclear Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Early cyst cell Cytoplasmic in early cyst cell Lower expression in tip region Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV ECM like pattern-SV Expression level-SV Expressed Medium - SV Cell type - SV Sperm Tail Decor - SV Cytoplasmic-SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Expression level-ED Expressed Low - ED Cell type - ED Nuclear-ED Nuclear associated - Ejaculatory Duct Subnuclear - Ejaculatory Duct Nuclear Foci - Ejaculatory Duct Cytoplasmic-ED Membrane enriched - ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Nuclear-SC Nuclear Foci-SC Cytoplasmic-SC Multivesicular bodies - SC Main cells -AG Nuclear- MC Nuclear foci-mc Cytoplasmic-MC Cytoplasmic Foci - AG |
Comments
| The probe may be recognizing 16 transcripts - Including flamenco. The probe may recognize some other areas in the genome that contain a highly homologous sequence. |
| Amazing patterns! |
| Very interesting patterns in adult testes and associated tissues. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Yolk Nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Weak expression (>41ms exp) Localized Yolk plasm localization Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Expression in API Expression in amnioserosa Dorsal blastoderm nuclei Medium expression (21-40ms exp) Localized Yolk plasm localization Embryonic localization patterns Ventral localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Tracheal lumen Specific tracheal branches Dorsal trunk Segmented pattern Salivary glands Proventriculus Pharynx Head Head epidermis Foregut Ectoderm Amnioserosa Amnioserosa edges Medium expression (21-40ms exp) |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -diverse ectoderm derived structures such as the salivary gland (and the salivary primordium), amnioserosa, proventriculus, tracheal pits, and epidermis |
| -prior reported expression of hemocytes were not visible in this experiment |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Localized Subcellular localization patterns Dorsal localization Basal localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in ectoderm Localized Embryonic localization patterns Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in ectoderm Localized Embryonic localization patterns Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Head Head epidermis Ectoderm CNS Ventral nerve cord Polarized VNC Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
| -Lateral and ventral spots in the epidermis of late stage samples are likely imaginal discs |
Probe E-spl-malpha-BFM-RA Links: Permalink
Genes: E(spl)malpha-BFM
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Localized Subcellular localization patterns Ventral localization Basal localization Basal enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Localized Embryonic localization patterns Ventral localization Germ band localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Muscles-embryo Visceral mesoderm Head Head epidermis Ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -Our experiment did not observe the same late stage patterns observed by BDGP (i.e. dorsal vessel, tracheal system, certain epidermal signals) |
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in all nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in all nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Accumulation in all nuclei Embryonic localization patterns Dorsal localization Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Head Head epidermis Ectoderm Brain Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Strong expression (1-20ms exp) |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Strong expression (1-20ms exp) |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Ubiquitous Strong expression (1-20ms exp) Localized Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Muscles-embryo Visceral mesoderm Midgut Head Head epidermis Ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Ubiquitous Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Ubiquitous Degraded Degraded partially stage 7 |
| stage 8-9 |
|
Stage 8-9 Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Salivary glands Pharynx Muscles-embryo Maxillary complex Hindgut Specific hindgut area or cells Head Head epidermis Ectoderm Anal pad Ring around anal pad Ubiquitous Localized Subcellular localization patterns Perinuclear localization Perinuclear around salivary gland nuclei Perinuclear around muscle nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in head Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RE transcript isoform |
| -very neat germ band stage and late stage patterns; salivary gland pattern may be real |
| -images are from two separate experiments under identical conditions for better coverage |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Apical localization Apical enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Apical localization Apical enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Salivary glands Head Head epidermis Ectoderm Polarized Ectoderm Atrium Localized Subcellular localization patterns Apical enrichment Apical in other tissues Apical ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Expressed in hindgut Subcellular localization in hindgut Nuclear in hindgut Hindgut - subnuclear localization Hindgut membranes Hindgut foci Few hindgut foci Hindgut basal enrichment Nascent transcript in hindgut |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - nuclear dots Nascent transcript in salivary gland Expressed in salivary glands Salivary glands allover |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Not expressed in larval muscles - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel - a bit low. Good in situ. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Yolk cortex localization Cytoplasmic foci Degraded Degraded partially stage 5 |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Proventriculus Midgut Midgut subset Hindgut Head Head epidermis Germ cell nuclei Gonads Garland cell Fat body Ectoderm Brain Localized Subcellular localization patterns Foci Small Foci Small Foci in midgut Small Foci in hindgut Small Foci in head Apical enrichment Apical enriched midgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RC isoform transcript |
| -BDGP reported pericardial cell which was not observed in this experiment |
| -Expression seen in garland cells or proventriculus or both, difficult to distinguish between one or the other |
| -Faint expression seen in posterior and lateral epidermis |
Probe Hsromega-ex Links: Permalink
Genes: Hsr-omega
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Yolk nuclei Subset yolk nuclei Ventral yolk nuclei Posterior yolk nuclei Anterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Ventral localization Posterior localization Exclusionary patterns Pole cell exclusion Basal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Localized Posterior localization Embryonic localization patterns Ventral localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Strong expression (1-20ms exp) Localized Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Midgut Head Head epidermis Ectoderm CNS Ventral nerve cord Brain Amnioserosa Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
| -lcnRNA |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Head Head epidermis CNS Ventral nerve cord Foci - VNC Amnioserosa Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Head Head epidermis Ectoderm Dorsal vessel CNS Ventral nerve cord Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
| -Posterior "circles" seen at approximately stages 13/14 may be either gonads or spiracles, or may be primordial Malpighian tubules |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Embryonic localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Posterior spiracles Muscles-embryo Muscles - subregion Maxillary complex Head Head epidermis Ectoderm Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -There is no entry for this gene on BDGP, and modENCODE RNAseq data on FlyBase indicates that it is not expressed in the embryo. However, we observed obvious patterns during embryogenesis. Additionally, FlyBase does not have any in-depth characterizations of this gene. |
| -Osi21 does not overlap with any other genes, ruling out the possibility of the targeting of alternative transcripts. BLAST also confirms the specificity of our probes to Osi21. Additionally, no other genes for which probes were designed for on this plate show patterns such as those observed here, ruling out the possibility of cross-contamination during probe preparation. |
| -This may be a result of Osi21's poor characterization or incorrect RNAseq data |
Probe TP53INP-ex Links: Permalink
Genes: TP53INP
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei All yolk nuclei Ubiquitous |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Expression in amnioserosa Ubiquitous Localized Embryonic localization patterns Ventral localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Sensory system Segmented pattern PNS Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Head Head epidermis Ectoderm CNS Ventral nerve cord Brain Amnioserosa Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Posterior localization Embryonic localization patterns Anterior localization Degraded Degraded partially stage 6 |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Localized Embryonic localization patterns Posterior localization Germ band localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Midgut subset Head Head epidermis Ectoderm Dorsal vessel CNS Ventral nerve cord Foci - VNC Brain Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Ubiquitous Localized Subcellular localization patterns Ventral localization Dorsal localization Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Head Head epidermis Ectoderm CNS Ventral nerve cord Foci - VNC Brain Localized Subcellular localization patterns Foci Small Foci Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Localized Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Head Head epidermis Ectoderm CNS Ventral nerve cord Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Head Head epidermis Ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Localized Subcellular localization patterns Ventral localization Basal localization Basal enrichment Degraded Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Basal localization Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Posterior localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Neuroblasts Polarized Neuroblasts Head Head epidermis Ectoderm CNS Ventral nerve cord Polarized VNC Perinuclear VNC Brain Localized Subcellular localization patterns Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -neat marker for neural precursor formation, appears to not be expressed past stage 13-14 except for 4 small points near the dorsal-posterior region of the ectoderm (possibly tip cells of the Malpighian tubules); clear stomatogastric nervous system primordium expression |
| -images are from two separate experiments under identical conditions for better coverage |
Probe betaTub56D-RA Links: Permalink
Genes: betaTub56D
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Localized Yolk cortex localization Yolk cortex enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Localized Yolk cortex localization Embryonic localization patterns Germ band localization Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Posterior spiracles PNS Muscles-embryo Muscles - subregion Hindgut Head Head epidermis Germ cell nuclei Gonads Ectoderm CNS Ventral nerve cord Clypeo-labral bud Brain Atrium Anal pad Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Foci Small Foci Small Foci in head Small Foci in Brain Basal enrichment Basal (outside) in brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -gorgeous epidermal patterns, also expressed in CNS, certain muscles, and gonads |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk cortex localization Yolk cortex enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Degraded Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in ectoderm Expression in API Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Head Head epidermis Ectoderm CNS Ventral nerve cord Axons Anal pad Localized Subcellular localization patterns Foci Small Foci Small Foci in hindgut Small Foci in head Small Foci in ectoderm Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -expression becomes weak in later stages but enriched foci are seen in several structures for a brief moment after dorsal retraction |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Dorsal localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Expression in API Localized Embryonic localization patterns Ventral localization Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Expression in API Localized Embryonic localization patterns Ventral localization Germ band localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Midgut Head Head epidermis Ectoderm CNS Ventral nerve cord Foci - VNC Axons Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -Most signals in the late stages are likely imaginal discs |
| -6 "ring"-like structures on the anterior ventral side are likely the leg discs -2 circles above leg discs on each side are likely wing discs -Band posterior to the VNC is likely the genital disc -small dots located on the late stage midgut may be intestinal stem cells (ISCs) |
| -Other signals in late stages likely correspond to imaginal discs which we cannot identify based on our resolution |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Segment polarity pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Expression in API Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in mesoderm Expression in ectoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Proventriculus Pharynx Hindgut Specific hindgut area or cells Head Head epidermis Esophagus Ectoderm Clypeo-labral bud Localized Subcellular localization patterns Nuclei-associated localization Nuclear in proventriculus Nuclear in pharynx Nuclear in ectoderm Foci Small Foci Small foci in Proventriculus Small Foci in Pharynx Small Foci in hindgut Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Strong expression (1-20ms exp) Localized Subcellular localization patterns Yolk plasm localization Ventral localization Dorsal localization Cytoplasmic foci Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Expression in ectoderm Strong expression (1-20ms exp) Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Strong expression (1-20ms exp) Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Germ band localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Hindgut Specific hindgut area or cells Head Head epidermis Ectoderm Anal pad Ring around anal pad Strong expression (1-20ms exp) |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Strong expression (1-20ms exp) Localized Subcellular localization patterns Ventral localization Exclusionary patterns Pole cell exclusion Dorsal localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Strong expression (1-20ms exp) Localized Embryonic localization patterns Ventral localization Exclusionary patterns Pole cell exclusion Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Strong expression (1-20ms exp) Localized Embryonic localization patterns Ventral localization Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Segmented pattern PNS Optic lobes primordium Head Head epidermis Ectoderm CNS Brain Strong expression (1-20ms exp) |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Maternal Degraded Degraded partially stage 4 |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 7 Degraded partially stage 6 |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Ubiquitous Localized Embryonic localization patterns Ventral localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Head Head epidermis Ectoderm CNS Ventral nerve cord Polarized VNC Brain Localized Subcellular localization patterns Basal enrichment Basal (outside) in CNS Basal (outside) in brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Localized Embryonic localization patterns Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Expression in API Localized Embryonic localization patterns Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Head Head epidermis Foregut Ectoderm Polarized Ectoderm Dorsal vessel |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RF isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Segment polarity pattern Pair-rule pattern Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Ventral localization Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Dorsal localization Cytoplasmic foci Apical localization Apical enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Posterior blastoderm nuclei Expression in ectoderm Dorsal blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Apical localization Apical enrichment Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Degraded Degraded partially stage 7 |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Dorsal blastoderm nuclei Degraded Degraded partially stage 8 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Maxillary complex Head Head epidermis Clypeo-labral bud Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -retested recently for late stage coverage (2025); appears to be expressed in a different program in the gnathal elements and sensory regions in late stages |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Basal localization Apical localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in mesoderm Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Ventral localization Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Single stripe Expression in ectoderm Anterior blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Sensory system Posterior spiracles Muscles-embryo Visceral mesoderm Hindgut Head Head epidermis Dorsal vessel CNS Ventral nerve cord Foci - VNC Axons Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Foci Small Foci Small Foci in PNS Small Foci in head Small Foci in ectoderm Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -neat and diverse expression patterns throughout development |
| -images are from two separate experiments under identical conditions for better coverage |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Ubiquitous Maternal Localized Yolk plasm localization Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Embryonic localization patterns Ventral localization Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Embryonic localization patterns Ventral localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Ring gland Head Head epidermis Ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 6 probe sets targeting the RA isoform transcript (previously tested with 3 and signals bordered between expressed and non-expressed) |
| -exceptional expression patterning in early epidermis and ring gland |
| -probes are from two different plates (3 sets from each plate); SH-HCR-Plate-1 and SH-HCR-Plate-4 |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Yolk nuclei Subset yolk nuclei Random yolk nuclei Ubiquitous Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Basal localization Basal enrichment Degraded Degraded partially stage 4 Degradation zygotic transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Posterior blastoderm nuclei Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Expression in neuroblasts Expression in ectoderm Expression in API Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern PNS Optic lobes primordium Neuroblasts Polarized Neuroblasts Midgut Maxillary complex Head Head epidermis CNS Ventral nerve cord Polarized VNC Perinuclear VNC Foci - VNC |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -expression seen in the stomatogastric sensory primordium, and the anterior and posterior midgut primordium; forms a complex along with ac (CG3796) so should colocalize in neuronal regions |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Segmented pattern Posterior spiracles Head Head epidermis Ectoderm Polarized Ectoderm Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Foci Small Foci Small Foci in ectoderm Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 6 probe sets targeting the RD isoform transcript (previously tested with 3 and was not detected) |
| -probes are from two different plates (3 sets from each plate); SH-HCR-Plate-1 and SH-HCR-Plate-4 |
| -this gene is very weakly expressed and more probe sets (if using HCR-FISH) should be considered for future retests |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Yolk plasm localization Yolk cortex localization Nuclei-associated localization Intranuclear accumulation Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Discrete apical foci Degraded Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in mesoderm Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Optic lobes primordium Neuroblasts Maxillary complex Head Head epidermis Ectoderm Dorsal vessel CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in ectoderm Nuclear in CNS Foci Small Foci Small Foci in head Small Foci in ectoderm Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -very diverse and neat patterns at all stages |