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Result Summary
| Number of genes | 28 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Cytoplasmic foci |
| stage 10-17 |
|
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Partly nascent, partly sub-nuclear. Transcript on 3R. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Pole cell localization Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Central band of nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Posterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Degraded Degraded partially stage 5 |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Nuclear dots. |
| Nuclear dots. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Dorsal yolk nuclei Germ cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Germ cell localization Germ cell enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Salivary glands PNS Pharynx Hemocytes Germ cell nuclei Gonads Ectoderm CNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Perinuclear around hemocytes Nuclei-associated localization Nuclear in PNS Nuclear in hemocytes Nuclear in ectoderm Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| The probe looks good on gel. |
| Dynamic expression. Salivary glands, probably non-specific |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Dorsal blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Dorsal localization Cytoplasmic foci Apical localization Apical enrichment Apical clusters Degraded Degraded partially stage 5 |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Expression in ectoderm Dorsal blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts Expression in mesoderm Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands Head Blastoderm nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe on gel Nice in situ! |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Germ cell nuclei All pole cell nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Cytoplasmic foci Germ cell localization Germ cell enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Germ cell nuclei Gonads Localized |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs Borderline between 'not expressed' and 'low expression' |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Not expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Gonads. |
| Pole cell specific. |
| Good probe on gel ---- Borderline between 'not expressed' and 'low expression' |
| Borderline between 'not expressed' and 'low expression' |
| Borderline between 'not expressed' and 'low expression' |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei All pole cell nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Ubiquitous Strong expression (1-20ms exp) Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei Foci Small Foci Small Foci in midgut Small Foci in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei All blastoderm nuclei Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Cell division apparatus Other cell division pattern Basal localization Basal enrichment Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in all nuclei Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in ectoderm Expression in amnioserosa Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Ectoderm CNS Ventral nerve cord Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Nuclear in CNS Nuclear in amnioserosa |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Midgut allover Expressed in hindgut Hindgut allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Cytoplasmic foci Expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Distal tip region-TE Not Expressed in distal tip region -TE Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Individualizing Spermatids - TE Waste Bag - TE Cytoplasmic in individualizing spermatids - TE Cyst cells -TE Tail cyst cell - TE Retracting/Coiling Tail Cyst Cell - TE Retracting/Coiling Tail Cyst Cell Cytoplasm - TE Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed High - TE Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Expression level-SV Expressed High-SV Cell type - SV Cytoplasmic-SV Membrane enriched - SV Cytoplasmic Foci - SV Ejaculatory Duct Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Cytoplasmic foci-SC Main cells -AG Cytoplasmic-MC |
Comments
| All nuclei. Strong in the Amnioserosa. |
| Enriched in the amnioserosa, the central nervous system, the ventral midline, and maybe the tracheal pits. |
| Anterior to posterior gradient Subnuclear localization. |
| The probe is low, but good. Nice insitu data!!! |
Probe CR43264-RA Links: Permalink
Genes: CR43264
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Yolk plasm localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Medium expression (21-40ms exp) Localized Yolk plasm localization Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Pole cell localization Pole cell enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Medium expression (21-40ms exp) Localized Yolk plasm localization Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Cytoplasmic foci Germ cell localization Germ cell enrichment Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Perinuclear around yolk nuclei Germ cell nuclei Gonads Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Foci Small Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 2 probe sets targeting the RA isoform transcript |
| -this non-coding gene is an extremely highly expressed lncRNA in male testes according to scRNA-seq data, and exhibits a strong nuclear pattern in in embryo yolk, pole cell, and random blastoderm cells |
Probe CR43314-RA Links: Permalink
Genes: CR43314
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Central band of nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Pole cell exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Anterior blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Expression in API Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Posterior spiracles Specific posterior spiracle pattern PNS Midgut Midgut subset Head Head epidermis Clypeo-labral bud Anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in midgut nuclei Nuclear in ectoderm Foci Small Foci Small Foci in PNS Small Foci in ectoderm Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
| -images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Yolk plasm localization |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Yolk cortex localization Exclusionary patterns Yolk plasm exclusion Degraded Degraded partially stage 4 |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm All blastoderm nuclei Persistent widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern PNS Maxillary complex Hindgut Germ cell nuclei Gonads CNS Ventral nerve cord Perinuclear VNC Axons Clypeo-labral bud Anal pad Ubiquitous Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -very diverse expression patterns in the late embryos; possibly expressed in the germ cell |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Pole cell localization Membrane associated Cell junction associated Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Membrane associated Cytoplasmic foci Basal localization Basal enrichment Apical localization Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Germ cell nuclei Gonads Ectoderm CNS Ventral nerve cord Blastoderm nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Receptor. |
| Good probe on gel |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in all nuclei Exclusionary patterns Apical exclusion Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Ventral yolk nuclei Anterior yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern All blastoderm nuclei Persistent widespread Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Germ cell nuclei Gonads Foregut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Foci Small Foci Small Foci in head |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Muscles-embryo Visceral mesoderm Head Localized Subcellular localization patterns Nuclei-associated localization Nuclear in muscle cells nuclei Foci Small Foci Small Foci in muscle cells Small Foci in head |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RI isoform transcript; difficult to pick best isoform to design probes to due to vast number of them, hard to say if these probes captured the complete expression profile for this gene |
| -Our previous experiments using TSA and independent experiments by BDGP observed maternal expression in stages 1-3 and stages 4-5, this was not observed in this experiment |
| -Interesting early mesoderm marker |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Embryonic localization patterns Ventral localization Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'podia' Perinuclear around yolk nuclei Fat body Strong expression (1-20ms exp) Localized Subcellular localization patterns Foci Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -retested 2025 for better late stage coverage of patterns |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Yolk nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Anterior blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Hemocytes Head Ectoderm CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in hemocytes Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Dots inside the nuclei. |
| Tracheal pits, yolk nuclei, some anterior zygotic expression, anterior midgut... Very dynamic and interesting. |
| Nuclear localization. |
| Low probe on gel' BcDNA:RE43210 |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Expression in API Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Apical exclusion Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Muscles-embryo Midgut Midgut subset Malpighian tubules CNS Ventral nerve cord Nuclear - VNC Foci - VNC Brain Localized Subcellular localization patterns Nuclei-associated localization Nuclear in hindgut nuclei Nuclear in CNS Foci Small Foci Small Foci in PNS Small Foci in midgut Small Foci in Malpighian tubules Small Foci in CNS Basal enrichment Basal in midgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -strong posterior midgut primordium expression |
| -possible expression in sensory organs in head and posterior epidermis |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in amnioserosa All blastoderm nuclei Persistent widespread Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Inside Yolk nuclei Midgut Hindgut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Yolk cortex exclusion |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Apical exclusion Local terms for zygotic transcripts Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Posterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Accumulation entire nucleus Local terms for zygotic transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Accumulation entire nucleus Local terms for zygotic transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Head CNS Ventral nerve cord Nuclear - VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in CNS Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Borderline between 'not expressed' and 'low expression' |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Not expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Re-annotated RW |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in ectoderm Ubiquitous Localized Subcellular localization patterns Perinuclear localization Perinuclear around all nuclei Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Neuroblasts CNS Ventral nerve cord Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH (3 probe sets) |
| -isoform corn-RC transcript was targeted |
| -weak expression in the late embryos that appear as small points along the ectoderm of the each segment (trunk and abdominal) located dorsolateral. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Yolk Nuclei Subset yolk nuclei Random yolk nuclei Localized |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'podia' Inside Yolk nuclei Posterior spiracles Muscles-embryo Visceral mesoderm Midgut Hindgut Midgut hindgut border Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Nuclear expression. Dots inside all yolk nuclei. |
| Salivary glands, may be non-specific. |
| Low probe on gel... good insitu |
| It is more 'cytoplasmic foci around pole cells'. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Segment polarity pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Expression in API Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in mesoderm Expression in ectoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Proventriculus Pharynx Hindgut Specific hindgut area or cells Head Head epidermis Esophagus Ectoderm Clypeo-labral bud Localized Subcellular localization patterns Nuclei-associated localization Nuclear in proventriculus Nuclear in pharynx Nuclear in ectoderm Foci Small Foci Small foci in Proventriculus Small Foci in Pharynx Small Foci in hindgut Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Right flanking nuclei Left flanking nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Right flanking nuclei Left flanking nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Segment polarity pattern Expression in neuroblasts Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Perinuclear around yolk nuclei Tracheal system Tracheal pits Segmented pattern Neuroblasts CNS Ventral nerve cord Polarized CNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei All cortical nuclei Strong expression (1-20ms exp) |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Local terms for zygotic transcripts Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Local terms for zygotic transcripts Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Local terms for zygotic transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Head CNS Ventral nerve cord Nuclear - VNC Brain Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in other cells Nuclear in ectoderm Nuclear in CNS Nuclear in amnioserosa Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus perinuclear Proventriculus perinuclear - few cells Expressed in midgut Subcellular localization in midgut Midgut - Nuclear Midgut - Subnuclear Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - Nuclear in Malpighian tubules - Subnuclear in Expressed in hindgut Subcellular localization in hindgut Nuclear in hindgut Hindgut - subnuclear localization Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca - nuclear localization Gastric caeca - subnuclear localization Nascent transcript in gastric caeca Expressed in esophagus |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - nuclear localization Ventral nerve cord - subnuclear localization Expressed in brain lobes Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - nuclear dots Nascent transcript in salivary gland |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Specific testis expression localization Larval testis foci |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva |
| tracheal system |
|
3rd Instar Larval Tissues Tracheal System Expressed in tracheal tubes |
| lymph glands and ring glands |
|
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Testicular duct-TE Nuclear in Testicular Duct - TE Terminal Epithelium - TE Nuclear in terminal epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatogonium-TE Nuclear in spermatogonium-TE Spermatocytes -TE Nuclear in Spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Individualizing Spermatids - TE Waste Bag - TE Cytoplasmic in individualizing spermatids - TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Pigment cells -TE Nuclear in pigment cells -TE Muscle cells - TE Nuclear in muscle cells -TE Cytoplasmic in muscle cells - TE Cyst cells -TE Tail cyst cell - TE Individualizing Tail Cyst Cell - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Nuclear Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Germline stem cell Cytoplasmic in germline stem cells - TE Early cyst cell Cytoplasmic in early cyst cell Clusters in early cyst cell-TE Cyst stem cell Cytoplasmic in cyst stem cell Lower expression in tip region Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Expression level-SV Expressed Medium - SV Cell type - SV Nuclear-SV Cytoplasmic-SV Perinuclear - SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Expression level-ED Expressed Medium - ED Cell type - ED Nuclear-ED Cytoplasmic-ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Diffuse foci-AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Nuclear-SC Nuclear Foci-SC Cytoplasmic-SC Multivesicular bodies - SC Main cells -AG Nuclear- MC Nuclear foci-mc Cytoplasmic-MC Cytoplasmic Foci - AG |
Comments
| Nuclear |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Segmented pattern Posterior spiracles Head Head epidermis Ectoderm Polarized Ectoderm Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Foci Small Foci Small Foci in ectoderm Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 6 probe sets targeting the RD isoform transcript (previously tested with 3 and was not detected) |
| -probes are from two different plates (3 sets from each plate); SH-HCR-Plate-1 and SH-HCR-Plate-4 |
| -this gene is very weakly expressed and more probe sets (if using HCR-FISH) should be considered for future retests |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Yolk plasm localization Yolk cortex localization Nuclei-associated localization Intranuclear accumulation Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Discrete apical foci Degraded Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in mesoderm Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Optic lobes primordium Neuroblasts Maxillary complex Head Head epidermis Ectoderm Dorsal vessel CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in ectoderm Nuclear in CNS Foci Small Foci Small Foci in head Small Foci in ectoderm Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -very diverse and neat patterns at all stages |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Sensory system Salivary glands Salivary glands - specific region PNS Ectoderm CNS Ventral nerve cord Nuclear - VNC Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs few cells - dots Leg discs allover Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc few cells - dots Expressed in antennal disc |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Ventral nerve cord - allover Expressed in brain lobes Optic lobe Brain lobes - Foci or dots Borderline between 'not expressed' and 'low expression' |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Expressed in salivary glands Salivary glands allover |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Nervous system. Nuclear. |
| Central nervous system. |
| Nuclear. Nervous system. |
| Good probe on gel |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in CNS Accumulation nuclear subregion Foci Small Foci Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Few gastric caeca foci Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Lymph Glands Expressed in lymph glands Lymph glands all over |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Spermatocyte Tail Cyst Cell - TE Spermatocyte Tail Cyst Cell Cytoplasm - TE Round Tail Cyst Cell - TE Round Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Early cyst cell Cytoplasmic in early cyst cell Seminal Vesicle Subcellularly localized - SV Lumenal-SV Non-particulate - SV Cell type - SV Cytoplasmic-SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Foci - ED Scattered Foci - ED Anterior ejaculatory duct papilla-ED Cytoplasmic in anterior ejaculatory papilla-ED Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Low - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC Cytoplasmic foci-SC Main cells -AG Cytoplasmic-MC Cytoplasmic Foci - AG |
Comments
| Good probe on gel. |
| Specific neuroblast expression. |
| Some photos are from the 2006 initial screen. More photos added in 2014 and many more terms. |