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Number of genes | 30 | |
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Salivary glands, probably non-specific. |
The probe looks good on gel The photos are from a combination of 2 experiments. One had a brighter probe than the other, which explains why some photos are brighter than others. |
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Good probe on gel. |
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The probe looks good in our gel. |
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Very low probe on gel. Good insitu hybridization. |
Interesting localization to yolk nuclei. |
Big 'foci' at the ventral nerve cord - not nuclear. |
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Good probe on gel ---- See map: the probe may be reporting for sprint as well... |
Specific zygotic expression: amnioserosa, pole cells and maybe some posterior midgut as well. |
Midgut, salivary glands. |
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Good probe on gel. |
Very interesting nascent pattern. I don't see sub-cellular localization. |
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Good probe on gel - a bit low. Good in situ. |
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Salivary glands, probably non-specific. |
Lots of specific expression, dynamic and strong. |
Good probe in our gel. |
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Low probe on gel' |
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Good probe on gel. |
Partly nascent, partly sub-nuclear. |
Maybe Partly nascent, partly sub-nuclear. |
Salivary glands, probably non-specific. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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Yolk nuclei |
Yolk nuclei |
Good probe on gel. |
Partly nascent, partly sub-nuclear. |
Good probe on gel. |
Specific zygotic expression. |
Partly nascent, partly sub-nuclear. |
Basal part of nuclei. Very interesting. |
Nuclear, subnuclear. |
Good probe on gel. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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Home made probe to last exon. Covers all isoforms. |
Salivary glands and tracheal lumen, probably non-specific. |
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Re-annotated with few changes. |
Maternal RNA excluded from the apical cytoplasm and pole cells, and gets degraded by stage 5 Zygotic RNA expressed from blastoderm and yolk nuclei Zygotic RNA seems to accumulate transiently in expressing blastoderm nuclei. |
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Dots inside the nuclei. |
Tracheal pits, yolk nuclei, some anterior zygotic expression, anterior midgut... Very dynamic and interesting. |
Nuclear localization. |
Low probe on gel' BcDNA:RE43210 |
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Re-annotated |
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Interesting nervous system expression, including the ventral midline. Salivary glands, probably non-specific. Partly nascent, partly sub-nuclear. |
Low probe on gel. Enough for a nice in situ... |
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The probe looks good, not bright. |
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Low probe on gel. |
Specific zygotic expression. |
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-performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
-interesting subcellular localization patterns in the pole/germ cells; makes a great marker for tracking the development of germ line cells throughout the entirety of embryogenesis |
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Ventral nerve cord, posterior midgut. |