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Number of genes | 38 | |
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Good probe on gel, a bit low. Very nice insitu... interesting patterns. |
Membrane localization apically, in small clusters. Very interesting. |
Membrane localization apically, in clusters. Very interesting. |
Salivary glands, probably non-specific. |
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I see sub-nuclear localization. |
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Good probe on gel |
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Salivary glands and tracheal lumen, probably non-specific. |
Good probe on gel. |
Yolk plasm 'fiber-like' expression, close to the yolk nuclei. |
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Good probe on gel |
Please note: Interesting small foci close to the pole cells at stage 5! |
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I see big 'dots' close to some balstoderm nuclei. It may be the centrioles (this gene is involved in centriole replication). |
I see big 'dots' close to some balstoderm nuclei (may be centrioles). |
Sensory complexes, visceral mesoderm. |
Low probe. |
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Probe looks good on gel. |
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Very low probe on gel. Good insitu hybridization. |
Interesting localization to yolk nuclei. |
Big 'foci' at the ventral nerve cord - not nuclear. |
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Low probe on gel' |
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RNA rings close to yolk nuclei. Very interesting pattern. |
Good probe RNA rings close to yolk nuclei. Interesting pattern. |
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RNA exhibits cortical enrichment and posterior pole plasm localization. |
RNA exhibits pole cell enrichment and is enriched apically in the posterior blastoderm nuclei. |
Salivary glands, my be non-specific. |
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Good probe on gel. |
Salivary glands, probably non-specific. Specific atrium localization. |
Very interesting pattern, I am mounting more embryos to see if I can find it again... |
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Good probe on gel. |
Maybe enriched at the posterior. |
Good probe on gel |
Re-annotated |
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Foci around pole cells. |
Good probe on gel. Max Niit took many of these photos. |
Larval testis: Borderline between 'not expressed' and 'low expression'. |
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Probe on gel a bit low. Good in situ! |
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Re-annotated. |
Clear Foci around pole cell nuclei. |
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One dot per nuclei, outside the nuclei (very close) |
Low probe on gel. |
Nuclear associated dot/foci. |
Membrane dots/foci and cytoplasmic dots/foci |
Good probe on gel, a bit low. Small, clear foci. |
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Good probe |
Some nuclei have one bright dot, not zygotic (this gene is on 2L). Structural constituent of the nucleopore. |
Some nuclei have one bright dot, not zygotic (this gene is on 2L). Structural constituent of the nucleopore. |
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The membrane localization seems to change with the cell cycle (interesting). |
Dorsal vessel specific. |
Good probe on gel. |
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Salivary glands, probably non-specific. |
Very interesting! Enriched posterior, not in the pole cells and with some basal sub-nuclear acumulation in posterior epithelia and in budding pole cells. |
Good probe on gel. Clear localization to the outside of the embryo with interesting patterns. |
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Good probe on gel. Interesting patterns. |
Apical localization. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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Male reproductive tissues |
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Good probe. Very nice insitu patterns! |
Data from RE10437 probe. |
Intra-nuclear in balstoderm and yolk nuclei. |
Intranuclear localization. Amnioserosa specific pattern. etc. Salivary glands and tracheal lumen, probably non-specific. |
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Good probe on gel. |
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Good probe on gel. |
I see 'cytoplasmic fibers' at the yolk plasm. |
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Good probe on gel. |
Very interesting expression |
I see strong peri-nuclear expression around the yolk nuclei, and 'podia' as well. |
Very interesting expression! |
Salivary glands, probably non-specific. |
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Good probe on gel. |
Only stage 5 embryos are recorded. We have previous data from spook at all stages of embryogenesis. Note: some 'yolk cell extensions' contain RNA. |
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'Egg-holder' pattern around the pole cells! |
'Egg-holder' pattern around the pole cells! |
Inside yolk nuclei. Salivary glands, probably non-specific. |
Lowish probe on gel. |
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Again, perinuclear pole cell asymmetric expression. |
Pole cell asymmetric expression. I see small 'dots' around the pole cells, stronger in one 1/2 of the pole nuclei. |
Good probe on gel |
Posterior localization, even in unfertilized eggs. RNA accumulates asymmetrically around the pole buds with a 'fiber-like' texture. When nuclei divide, the RNA will stay in the nuclei that are closer to the posterior end. Very interesting! |
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Good probe on gel. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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Good probe on gel. |