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Result Summary

Number of genes	39
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Probe GH23463 Links: Permalink

Genes: 18w

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Weak expression (>41ms exp) Localized
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Segmented pattern Head Blastoderm nuclei

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in gastric caeca Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues Nervous system Not expressed in nervous system
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Not expressed in lymph glands

Adult

Area	Images	Terms

Comments

insitu done in 2006 by Eric's group.

Probe RE13301 Links: Permalink

Genes: ASPP

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Other subcellular pattern Membrane associated Exclusionary patterns Pole cell exclusion Basal exclusion Dorsal localization Cytoplasmic foci Cell junction associated Apical localization Apical enrichment Discrete apical foci Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Other subcellular pattern Membrane associated Exclusionary patterns Yolk plasm exclusion Basal exclusion Cytoplasmic foci Cell junction associated Apical localization Apical enrichment Discrete apical foci Apical clusters Embryonic localization patterns Exclusionary patterns Pole cell exclusion Dorsal localization
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Other subcellular pattern Membrane associated Cytoplasmic foci Apical localization Apical enrichment Discrete apical foci Apical clusters Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Salivary glands Posterior spiracles Head Head epidermis Esophagus Ectoderm Polarized Ectoderm CNS Polarized CNS Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in CNS Accumulation nuclear subregion Foci Small Foci Small Foci in ectoderm Small Foci in amnioserosa Bigger Foci Apical enrichment Apical ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Individualizing Spermatids - TE Cystic bulge - TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Distal tip region-TE Not Expressed in distal tip region -TE Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Cell type - SV Cytoplasmic-SV Perinuclear - SV Membrane enriched - SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Membrane enriched - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Low - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC Main cells -AG Cytoplasmic-MC Membrane Enriched-MC

Comments

Good probe on gel, a bit low. Very nice insitu... interesting patterns.

Membrane localization apically, in small clusters. Very interesting.

Membrane localization apically, in clusters. Very interesting.

Salivary glands, probably non-specific.

Probe LD39923 Links: Permalink

Genes: Ama

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Apical localization Apical enrichment Diffuse apical localization Apical clusters Local terms for zygotic transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Dorsal blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Basal localization Apical localization Apical enrichment Apical clusters Local terms for zygotic transcripts
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in API Localized Subcellular localization patterns Apical localization Apical enrichment Local terms for zygotic transcripts

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc few cells - dots Basal wing disc localization Expressed in leg discs Leg discs few cells - dots Leg discs - outside Expressed in eye disc Eye disc photo-receptors Eye disc few cells - dots Eye disc basal Eye disc - center round cells
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Localized to axons Borderline between 'not expressed' and 'low expression'
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms

Comments

Re-annotated

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions'

Probe RH02410 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Germ cell nuclei Subset pole cell nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Muscles-embryo Midgut Blastoderm nuclei

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

One dot per nuclei at the pole cells (I think) and other cells.

I see some bright dots, one per nuclei. At the anterior end of some embryos, apically localized.

Low probe on gel.

Probe RE20251 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Exclusionary patterns Apical localization Apical enrichment Apical clusters Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Expression in amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Segmented pattern Oenocytes Muscles-embryo Visceral mesoderm Head Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Cytoplasmic foci-fat body Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in hindgut Hindgut allover Hindgut - Midgut border Expressed in gastric caeca Gastric caeca allover
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Optic lobe Brain lobes - Significant area Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - nuclear dots Salivary gland - Cytoplasmic foci Nascent transcript in salivary gland Expressed in salivary glands Salivary glands allover
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover

Adult

Area	Images	Terms

Comments

Good probe on gel.

Probe SD03723 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei All blastoderm nuclei Transiently widespread Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus Spindle midzone localization Chromatin associated metaphase Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Single stripe Expression in neuroblasts Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Ventral localization

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Re-annotated. Images from Eric's team.

Probe GH05918 Links: Permalink

Genes: scaf

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Sensory system Segmented pattern Proventriculus Posterior spiracles Specific posterior spiracle pattern PNS Pharynx Muscles-embryo Muscles - subregion Germ cell nuclei Gonads Ectoderm Dorsal vessel CNS Ventral nerve cord Perinuclear VNC Foci - VNC Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Perinuclear around CNS nuclei Foci Small Foci Small Foci in ectoderm Small Foci in CNS Bigger Foci Apical enrichment Apical ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues

Comments

Good probe on gel.

Interesting.

Nuclear-associated 'dot'. Not zygotic 2R.

Probe LD47795 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Apical localization Apical enrichment Apical clusters Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Maternal Localized Subcellular localization patterns Apical localization Apical enrichment Embryonic localization patterns Degraded Degraded partially stage 9 Degraded partially stage 8 Degradation maternal transcripts

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Not expressed in fat body
intestine		3rd Instar Larval Tissues Intestine Expressed in pharynx
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc apical
nervous system		3rd Instar Larval Tissues Nervous system Not expressed in nervous system
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland

Adult

Area	Images	Terms

Comments

Re-annotated

Probe LD40094 Links: Permalink

Genes: Girdin

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Cell division apparatus Other cell division pattern Centrosomal localization Embryonic localization patterns
stage 4-5		Stage 4-5 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Nuclei-associated localization Perinuclear localization Exclusionary patterns Pole cell exclusion Cell division apparatus Other cell division pattern Centrosomal localization Apical localization Apical enrichment Apical clusters Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci Apical localization Apical enrichment Apical clusters Pole cell localization Pole cell enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci Apical localization Apical enrichment Degraded Degraded partially stage 9
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Salivary glands Salivary glands lumen Pharynx Midgut Fat body Ectoderm Polarized Ectoderm CNS Ventral nerve cord Foci - VNC Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in Pharynx Small Foci in midgut Small Foci in fat body Small Foci in CNS Apical enrichment Apical enriched midgut Apical ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Apical localization. Nuclei associated. Posterior enriched. Pole cells excluded in some, not all. Posterior to anterior gradient.

Good probe on gel. Great patterns!

Probe AT21302 Links: Permalink

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Yolk nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

The probe looks good in our gel.

Probe GM09909 Links: Permalink

Genes: CG2947

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Apical localization Apical enrichment Apical clusters Degraded Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Maternal Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters
stage 8-9		Stage 8-9 Expressed Ubiquitous Maternal Degraded Degraded partially stage 8 Degradation maternal transcripts

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe GH07363 Links: Permalink

Genes: CG7192

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Other subcellular pattern Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion
stage 4-5		Stage 4-5 Expressed Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Non-Expressed Degraded Degraded completely stage 6
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Non-Expressed

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
nervous system		3rd Instar Larval Tissues Nervous system Not expressed in nervous system
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands Lymph glands all over

Adult

Area	Images	Terms

Comments

Good probe on gel Amazing localization, 'nuclear donuts'.

Probe AT29766 Links: Permalink

Genes: CG8197

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Dorsal blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Dorsal localization Cytoplasmic foci Apical localization Apical enrichment Apical clusters Degraded Degraded partially stage 5
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Expression in ectoderm Dorsal blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Embryonic localization patterns Ventral localization
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts Expression in mesoderm Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Sensory system Salivary glands Head Blastoderm nuclei

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Low probe on gel Nice in situ!

Probe HL04319 Links: Permalink

Genes: CG8538

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Apical localization Apical enrichment Diffuse apical localization Apical clusters Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in API Weak expression (>41ms exp) Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Blastoderm nuclei

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe LD23613 Links: Permalink

Genes: CycB

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Posterior localization RNA islands Pole plasm Pole buds Cell division apparatus Microtubule-associated Anterior localization Embryonic localization patterns
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Cell division apparatus Microtubule-associated Apical localization Apical enrichment Apical clusters Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Maternal Localized Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns Degraded Degraded partially stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed

Larva

Area	Images	Terms

Adult

Area	Images	Terms
Male reproductive tissues

Comments

Borderline between 'not expressed' and 'low expression'

Probe RE02264 Links: Permalink

Genes: ECSIT

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Pair-rule pattern Gap-gene pattern Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Weak expression (>41ms exp) Localized
stage 10-17		Stage 10-17 Expressed Zygotic Proventriculus Hindgut Specific hindgut area or cells Germ cell nuclei Gonads Ectoderm Blastoderm nuclei Localized

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Proventriculus allover Expressed in pharynx Pharynx particular pattern Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in gastric caeca Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Wing disc few cells - dots Wing disc allover Basal wing disc localization Expressed in leg discs Leg discs few cells - dots Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc few cells - dots Eye disc basal Eye disc apical Eye disc allover Eye disc - center round cells Expressed in antennal disc Antennal disc few cells - dots
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Borderline between 'not expressed' and 'low expression'
salivary gland		3rd Instar Larval Tissues Salivary gland Expressed in salivary glands Salivary glands allover Borderline between 'not expressed' and 'low expression'
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands Lymph glands all over

Adult

Area	Images	Terms

Comments

The wingless probe is on the next row of the same plate... Potential cross-contamination.

Good probe on gel.

Probe RE69770 Links: Permalink

Genes: HLHm5

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Right flanking nuclei Left flanking nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Cell junction associated Basal localization Apical localization Apical enrichment Apical clusters Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Right flanking nuclei Left flanking nuclei Expression in mesoderm Expression in ectoderm Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Intranuclear accumulation Exclusionary patterns Cell junction associated Basal localization Basal enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Medium expression (21-40ms exp)
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern PNS Ectoderm CNS Ventral nerve cord Brain Localized Subcellular localization patterns Membrane associated Cell junction associated

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe RE45036 Links: Permalink

Genes: Ptr

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Right flanking nuclei Left flanking nuclei Dorsal blastoderm nuclei Localized Subcellular localization patterns Cell division apparatus Spindle midzone localization Chromatin associated metaphase Apical localization Apical enrichment Apical clusters Local terms for zygotic transcripts Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Right flanking nuclei Left flanking nuclei Dorsal blastoderm nuclei Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters Local terms for zygotic transcripts Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Zygotically expressed RNA exhibits differents cell division associated patterns. The RNA seems to accumulate around the metaphase chromosomes and exhibits spindle midzone association. The RNA also acculates in the apical cytoplasm.

Probe LD09474 Links: Permalink

Genes: Rad21

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Yolk plasm localization Yolk plasm enrichment Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Basal localization Basal enrichment
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Midgut Head Blastoderm nuclei Amnioserosa Localized

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe GH03186 Links: Permalink

Genes: Sema-1b

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Single stripe Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Cytoplasmic foci Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Single stripe Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Single stripe Expression in neuroblasts Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Tracheal system Hindgut Specific hindgut area or cells Head Ectoderm CNS Ventral nerve cord Foci - VNC Clypeo-labral bud Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in hindgut Small Foci in ectoderm Small Foci in CNS Apical enrichment Apical ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good strong probe on gel.

Probe RE40347 Links: Permalink

Genes: Sry-alpha

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around all nuclei Degraded Degraded partially stage 3 Degraded partially stage 2 Degradation maternal transcripts
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Apical clusters Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Exclusionary patterns Apical localization Apical enrichment Apical clusters Embryonic localization patterns Exclusionary patterns Pole cell exclusion Degraded Degraded completely stage 7 Degradation zygotic transcripts
stage 8-9		Stage 8-9 Expressed Ubiquitous

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe LD05688 Links: Permalink

Genes: Tom

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Other subcellular pattern Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Dorsal blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Membrane associated Exclusionary patterns Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Membrane associated Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Ectoderm CNS Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Membrane associated Foci Small Foci Small Foci in ectoderm Small Foci in CNS Small Foci in Brain Cell junction associated Apical enrichment Apical ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Wing disc few cells - dots Expressed in leg discs Leg discs few cells - dots Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc few cells - dots Expressed in antennal disc Antennal disc few cells - dots
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms

Comments

Good probe on gel. Interesting patterns.

Apical localization.

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions.

Probe LD45157 Links: Permalink

Genes: bib

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Right flanking nuclei Left flanking nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Right flanking nuclei Left flanking nuclei Expression in ectoderm Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Apical enrichment Apical ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Not expressed in fat body
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc nuclear localization Eye disc subnuclear localization Eye disc few cells - dots Eye disc apical
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots'
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands Lymph glands all over

Adult

Area	Images	Terms

Comments

Re-annotated 2014

Probe GH12078 Links: Permalink

Genes: bnb

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cell division apparatus
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Gap-gene pattern Medium expression (21-40ms exp) Localized Subcellular localization patterns Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Cell division apparatus Apical localization Apical enrichment Diffuse apical localization Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Expression in ectoderm Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Apical localization Apical enrichment Diffuse apical localization Apical clusters Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in ectoderm Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Other subcellular pattern Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cell junction associated Apical localization Apical enrichment Germ cell localization Germ cell enrichment Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Segmented pattern Salivary glands Midgut Midgut subset Ectoderm Polarized Ectoderm Dorsal vessel CNS Ventral nerve cord Polarized VNC Perinuclear VNC Blastoderm nuclei Amnioserosa Amnioserosa edges Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around midgut nuclei Perinuclear around ectoderm nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in other cells Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus

Larva

Area	Images	Terms

Adult

Area	Images	Terms
Male reproductive tissues

Comments

Good probe. Very nice insitu patterns!

Data from RE10437 probe.

Intra-nuclear in balstoderm and yolk nuclei.

Intranuclear localization. Amnioserosa specific pattern. etc. Salivary glands and tracheal lumen, probably non-specific.

Probe GM04379 Links: Permalink

Genes: c(3)G

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Anterior localization Embryonic localization patterns
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization Exclusionary patterns Yolk plasm exclusion Basal exclusion Apical localization Apical enrichment Diffuse apical localization Apical clusters Anterior localization Degraded Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in ectoderm Expression in API Localized
stage 10-17		Stage 10-17 Expressed Zygotic Ectoderm CNS Ventral nerve cord Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Foci Small Foci Small Foci in other tissues Small Foci in CNS

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Central nervous system

Anterior gradient.

Probe RE56519 Links: Permalink

Genes: c(3)G

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Anterior localization Embryonic localization patterns
stage 4-5		Stage 4-5 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Apical clusters Anterior localization Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Strong expression (1-20ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns Degraded Degraded partially stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in API Expression in amnioserosa Strong expression (1-20ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Muscles-embryo Ectoderm CNS Ventral nerve cord Axons Localized

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Apical, anterior to posterior gradient.

Anterior to posterior gradient!

Probe LD13435 Links: Permalink

Genes: crb

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Right flanking nuclei Left flanking nuclei Dorsal blastoderm nuclei Localized Subcellular localization patterns Apical localization Apical enrichment Diffuse apical localization Apical clusters Local terms for zygotic transcripts Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters Local terms for zygotic transcripts Embryonic localization patterns
stage 8-9		Stage 8-9 Non-Expressed Degraded Degraded completely stage 8 Degradation maternal transcripts

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe RE20611 Links: Permalink

Genes: dpp

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Apical localization Apical enrichment Apical clusters Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Apical localization Apical enrichment Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Tracheal lumen Specific tracheal branches Dorsal trunk Neuroblasts Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Midgut Hindgut Head Ectoderm Blastoderm nuclei Atrium Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in other tissues Small Foci in midgut Small Foci in hindgut Small Foci in head Small Foci in ectoderm Small Foci in amnioserosa Apical enrichment Apical enriched hindgut

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Perinuclear localization Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Nascent transcript in proventriculus Expressed in midgut Subcellular localization in midgut Midgut - Nuclear Midgut - Subnuclear Nascent transcript in midgut Midgut allover Expressed in hindgut Hindgut - Midgut border Expressed in gastric caeca Gastric caeca allover
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area
ovary and testis		3rd Instar Larval Tissues Larval Ovary and Testis-Larva Ovary allover Expressed in Ovary
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva

Adult

Area	Images	Terms

Comments

Clear patches of cells close to the invaginating tracheal placodes.

Good probe on gel. RNA is allover, with some small but clear foci.

Probe RE61427 Links: Permalink

Genes: fra

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Dorsal blastoderm nuclei Central band of nuclei Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Apical localization Apical enrichment Apical clusters Local terms for zygotic transcripts Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Dorsal blastoderm nuclei Central band of nuclei Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Apical exclusion Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Medium expression (21-40ms exp)

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe LD32130 Links: Permalink

Genes: htl

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in mesoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Re-annotated

Probe LD32155 Links: Permalink

Genes: l(1)G0331

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around pole cell nuclei Perinuclear around cortical nuclei Cell division apparatus Microtubule-associated
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Cell division apparatus Microtubule-associated Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed
stage 8-9		Stage 8-9 Expressed

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe LD28033 Links: Permalink

Genes: lola

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around cortical nuclei Perinuclear around all nuclei
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Posterior yolk nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Cell junction associated Apical localization Apical enrichment Diffuse apical localization Apical clusters Local terms for zygotic transcripts Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Persistent widespread Localized Subcellular localization patterns Exclusionary patterns Apical localization Apical enrichment Diffuse apical localization Local terms for zygotic transcripts Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Localized Local terms for zygotic transcripts
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern CNS Ventral nerve cord Brain Blastoderm nuclei

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe LD44171 Links: Permalink

Genes: mu2

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk cortex localization Yolk cortex enrichment Exclusionary patterns Pole cell exclusion Cytoplasmic foci Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Exclusionary patterns Basal localization Basal enrichment Apical localization Apical enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Midgut Hindgut CNS Ventral nerve cord Foci - VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in midgut Small Foci in hindgut Small Foci in CNS Small Foci in Brain

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Anterior to posterior gradient.

Good probe on gel.

Probe GM04312 Links: Permalink

Genes: pan

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Yolk cortex exclusion Pole plasm excluded
stage 4-5		Stage 4-5 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Discrete apical foci Apical clusters
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in API
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in API Expression in amnioserosa Anterior blastoderm nuclei
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Head Ectoderm Brain Amnioserosa

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules few foci Malpighian tubules allover Expressed in hindgut Hindgut allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc few cells - dots Borderline between 'not expressed' and 'low expression'
salivary gland		3rd Instar Larval Tissues Salivary gland Expressed in salivary glands Salivary glands allover Borderline between 'not expressed' and 'low expression'
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover

Adult

Area	Images	Terms

Comments

Good probe on gel. Nice insitu.

Probe RE24064 Links: Permalink

Genes: ref(2)P

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around pole cell nuclei Perinuclear around all nuclei Cytoplasmic foci Embryonic localization patterns
stage 4-5		Stage 4-5 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Exclusionary patterns Basal exclusion Cytoplasmic foci Apical localization Apical enrichment Discrete apical foci Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Anterior blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Apical localization Apical enrichment Discrete apical foci Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Germ cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in API Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Nuclei-associated localization Cytoplasmic foci Germ cell localization Germ cell enrichment Degraded Degraded partially stage 9
stage 10-17		Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Salivary glands - specific region Posterior spiracles Hindgut Specific hindgut area or cells Midgut hindgut border CNS Ventral nerve cord Foci - VNC Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in CNS Bigger Foci Apical enrichment Apical enriched hindgut

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe Very interesting localization!

Nice!

Probe RE40068 Links: Permalink

Genes: sala

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 3 Degraded partially stage 2 Degradation maternal transcripts
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Strong expression (1-20ms exp) Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Apical clusters Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Expression in mesoderm Strong expression (1-20ms exp) Degraded Degraded completely stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in mesoderm Expression in ectoderm Strong expression (1-20ms exp)

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe RE22261 Links: Permalink

Genes: sob

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Pole cell localization Cytoplasmic foci Basal localization Apical localization Apical enrichment Diffuse apical localization Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern
stage 10-17		Stage 10-17 Expressed Zygotic Sensory system Segmented pattern Salivary glands Salivary glands lumen Head Ectoderm CNS Ventral nerve cord Localized

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel.

Nice stripes.

Probe LP09189 Links: Permalink

Genes: sog

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Right flanking nuclei Left flanking nuclei Dorsal blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Yolk plasm localization Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Cell division apparatus Apical localization Apical enrichment Diffuse apical localization Apical clusters Local terms for zygotic transcripts Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Right flanking nuclei Left flanking nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Cytoplasmic foci Cell division apparatus Apical localization Apical enrichment Apical clusters Local terms for zygotic transcripts Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Local terms for zygotic transcripts Embryonic localization patterns Ventral localization
stage 10-17		Stage 10-17 Expressed Zygotic CNS Ventral nerve cord Polarized VNC Foci - VNC Blastoderm nuclei Localized Subcellular localization patterns Membrane associated Foci Small Foci Small Foci in CNS Basal enrichment Basal (outside) in CNS

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Basal wing disc localization Apical wing disc localization Expressed in leg discs Leg discs few cells - dots Expressed in eye disc Eye disc apical Expressed in antennal disc Antennal disc few cells - dots Antennal disc - center round cells
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland

Adult

Area	Images	Terms

Comments

Good probe on gel.

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions.

Probe GH10871 Links: Permalink

Genes: trn

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole plasm excluded
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Medium expression (21-40ms exp) Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Apical localization Apical enrichment Apical Localization Neuroblasts
stage 10-17		Stage 10-17 Expressed Zygotic Sensory system Segmented pattern Salivary glands Salivary glands lumen Ring gland Proventriculus PNS Pharynx Midgut Midgut subset Hindgut Specific hindgut area or cells Head Foregut Ectoderm Polarized Ectoderm CNS Ventral nerve cord Perinuclear VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around ectoderm nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in ectoderm Accumulation entire nucleus