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Number of genes | 12 | |
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Good strong probe. Maybe 'small foci'. |
Not all zygotic, this gene is on 2R and I see -in many cases- one bright dot per nuclei. |
Interesting. |
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Good probe on gel. |
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-performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
-appears ubiquitously throughout development with some mesoectodermal enrichment during the germ band elongation phases (appears as stripes) |
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On the X chromosome. One dot inside the nuclei may mean nascent transcript on a male embryo. |
Interesting pattern... |
Good probe - low. |
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Need more images to get the full story. In situ done before we started screening late stage embryos. |
Weak probe on gel. |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
-images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
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-performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
-very diverse expression patterns in the late embryos; possibly expressed in the germ cell |
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Good strong probe on gel. |
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-performed using HCR-FISH (3 probe sets) |
-probes targeted dac-RB isoform of transcript |
-very neat patterning in later stages; possibly visceral muscles of the hindgut |
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Maybe some 'foci'. |
Nuclear - posterior spiracles Salivary glands, probably non-specific. |
Lowish probe on gel. Good in situ! |
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Salivary glands, probably non-specific. |
Good probe on gel. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-retested recently for late stage coverage (2025); appears to be expressed in a different program in the gnathal elements and sensory regions in late stages |