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Result Summary
| Number of genes | 39 | |
| Export | ||
Probe SD10530 Links: Permalink
Genes: BcDNA:SD10530
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Cell division apparatus Other cell division pattern Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation entire nucleus Germ cell localization Germ cell enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Posterior spiracles Pharynx Midgut Hemocytes Head Ectoderm Brain Localized Subcellular localization patterns Nuclei-associated localization Nuclear in pharynx Nuclear in other cells Nuclear in midgut nuclei Nuclear in hemocytes Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Nuclear and sub-nuclear localization. |
| Nuclear. |
| Nuclear and sub-nuclear localization. |
| Very nice pattern!! |
| Very low probe on gel. Great in situ... No transcript associated with this probe, it may be a copia transposon element. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Perinuclear around yolk nuclei Inside Yolk nuclei Esophagus Ectoderm Localized Subcellular localization patterns Perinuclear localization Nuclei-associated localization Nuclear in ectoderm Accumulation nuclear subregion Foci Small Foci Small Foci in ectoderm Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Partly nascent, partly sub-nuclear. |
| Lowish probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Pole cell localization Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Central band of nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Posterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Segmented pattern Salivary glands Posterior spiracles Pharynx Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in tracheal nuceli Nuclear in ectoderm Accumulation nuclear subregion Foci Small Foci Small Foci in tracheal cells Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Salivary glands, probably non-specific. Partly nascent, partly sub-nuclear |
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Yolk plasm localization Yolk plasm enrichment Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands lumen Pharynx Muscles-embryo Muscles - subregion Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around ectoderm nuclei Other subcellular pattern Nuclei-associated localization Nuclear in pharynx Nuclear in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Muscle sub-cellular (double band). Salivary glands and tracheal lumen, probably non-specific. |
| Good probe on gel. Borderline between 'not expressed' and 'low expression'. |
| Basal enriched. |
| Interesting late embryo patterns. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion |
|
| stage 6-7 |
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
|
| stage 8-9 |
Stage 8-9 Non-Expressed Degraded Degraded completely stage 8 |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Salivary glands Posterior spiracles Specific posterior spiracle pattern Head Head epidermis Ectoderm Polarized Ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Nuclei-associated localization Nuclear in ectoderm Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in hindgut Subcellular localization in hindgut Hindgut foci Hindgut allover |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Nice Embryo data |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Non-Expressed Degraded Degraded completely stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Non-Expressed |
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands - specific region Posterior spiracles Muscles-embryo Muscles - subregion Ectoderm Polarized Ectoderm Dorsal vessel Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| semi-good probe. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Strong expression (1-20ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Comments
| Good probe. Not sure if it is excluded at the pole plasm. |
| Need better images. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns |
| stage 6-7 |
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
|
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Posterior spiracles Pharynx Muscles-embryo Visceral mesoderm Muscles - subregion Midgut Midgut subset Midgut ICPs Hindgut Specific hindgut area or cells Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around muscle nuclei Perinuclear around midgut nuclei Nuclei-associated localization Nuclear in pharynx Nuclear in other cells Nuclear in midgut nuclei Nuclear in hindgut nuclei Nuclear in ectoderm Accumulation nuclear subregion Foci Small Foci Small Foci in muscle cells Small Foci in midgut Basal enrichment Basal in hindgut Apical enrichment Apical enriched hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Gap-gene pattern Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Tracheal system Segmented pattern Proventriculus Muscles-embryo Visceral mesoderm Hindgut Head Ectoderm Dorsal vessel CNS Ventral nerve cord Axons Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in tracheal nuceli Nuclear in ectoderm Accumulation nuclear subregion Foci Small Foci Small Foci in tracheal cells Small Foci in hindgut Small Foci in ectoderm Bigger Foci Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Partly nascent, partly sub-nuclear. Transcript comes from 3R. |
| Good probe on gel. All over with foci. |
| Partly nascent, partly sub-nuclear. Transcript comes from 3R. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in ectoderm Expression in API Weak expression (>41ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands lumen Posterior spiracles Pharynx Muscles-embryo Visceral mesoderm Midgut Hindgut Specific hindgut area or cells Foregut Esophagus Ectoderm Polarized Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around ectoderm nuclei Nuclei-associated localization Nuclear in ectoderm Foci Small Foci Small Foci in salivary glands Small Foci in hindgut Small Foci in ectoderm Apical enrichment Apical enriched hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe in gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei Anterior yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Tracheal system Salivary glands Salivary glands lumen Head Ectoderm CNS Ventral nerve cord Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Nuclei-associated localization Nuclear in tracheal nuceli Nuclear in ectoderm Accumulation nuclear subregion Foci Small Foci Small Foci in tracheal cells Bigger Foci |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Particular proventriculus area Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut - Few foci Midgut allover Expressed in hindgut Hindgut allover Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover Expressed in esophagus |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs few cells - dots Leg discs allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - nuclear dots Salivary gland - Cytoplasmic foci Nascent transcript in salivary gland Expressed in salivary glands Salivary glands allover |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Partly nascent, partly sub-nuclear. |
| Maybe Partly nascent, partly sub-nuclear. |
| Salivary glands, probably non-specific. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
|
Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Segmented pattern Salivary glands PNS Head Ectoderm Dorsal vessel Dorsal vessel specific pattern Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around ectoderm nuclei Perinuclear around dorsal vessel nuclei Nuclei-associated localization Nuclear in ectoderm Accumulation nuclear subregion Foci Small Foci Small Foci in ectoderm Small Foci in dorsal vessel Cell junction associated |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Very low expression. |
| Good probe on gel. Very low expression. |
| Partly nascent, partly sub-nuclear. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Posterior spiracles Specific posterior spiracle pattern Pharynx Ectoderm Polarized Ectoderm Blastoderm nuclei Atrium Anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Interesting posterior spiracles and pharynx patterns. |
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk plasm localization Posterior localization Pole cell localization Pole cell enrichment Exclusionary patterns Apical exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Maternal Localized Subcellular localization patterns Other subcellular pattern Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Maternal Localized Germ cell localization Germ cell enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Proventriculus Posterior spiracles Specific posterior spiracle pattern PNS Muscles-embryo Muscles - subregion Midgut Hindgut Foregut Ectoderm Blastoderm nuclei Atrium Localized Subcellular localization patterns Nuclei-associated localization Nuclear in muscle cells nuclei Nuclear in midgut nuclei Nuclear in hindgut nuclei Nuclear in ectoderm Accumulation nuclear subregion Foci Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Partly nascent, partly nuclear subregion. |
| done twice. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Dorsal yolk nuclei Germ cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Germ cell localization Germ cell enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Salivary glands PNS Pharynx Hemocytes Germ cell nuclei Gonads Ectoderm CNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Perinuclear around hemocytes Nuclei-associated localization Nuclear in PNS Nuclear in hemocytes Nuclear in ectoderm Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| The probe looks good on gel. |
| Dynamic expression. Salivary glands, probably non-specific |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen PNS Midgut Head Fat body Ectoderm Blastoderm nuclei Atrium Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in ectoderm Accumulation nuclear subregion Foci Small Foci Small Foci in ectoderm Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Salivary glands, probably non-specific Sub-nuclear localization. |
| Maybe lower at the pole cells and lower apical... |
| Low probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Gap-gene pattern Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Gap-gene pattern Expression in ectoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts Expression in ectoderm Expression in amnioserosa |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Posterior spiracles Midgut Head Ectoderm Polarized Ectoderm Dorsal vessel Dorsal vessel specific pattern Brain Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus Foci Small Foci Small Foci in dorsal vessel |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Brain, optic lobe, ventral nerve cord, head epidermis. |
| Epidermis, dorsal vessel. |
| Not at all stages it is 'Apical excluded' Nice Gap expression! |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in ectoderm Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands Salivary glands lumen Posterior spiracles Midgut Midgut subset Head Esophagus Ectoderm Dorsal vessel CNS Ventral nerve cord Axons Polarized CNS Brain Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Perinuclear around CNS nuclei Perinuclear 'rings' Nuclei-associated localization Nuclear in ectoderm Accumulation nuclear subregion Cell extensions or 'podia' Basal enrichment Basal in midgut Basal (outside) in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Basal localization Basal enrichment Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in ectoderm Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Salivary glands Proventriculus Midgut Fat body Ectoderm Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Foci Small Foci Small Foci in amnioserosa Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Maternal |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Posterior spiracles Hindgut Esophagus Ectoderm CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe. |
| Sub-nuclear (only one dot per nuclei in some tissues, like the posterior spiracles - 2L). Partly nascent, partly sub-nuclear. Tracheal lumen, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei All blastoderm nuclei Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Cell division apparatus Other cell division pattern Basal localization Basal enrichment Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in all nuclei Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in ectoderm Expression in amnioserosa Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Ectoderm CNS Ventral nerve cord Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Nuclear in CNS Nuclear in amnioserosa |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Midgut allover Expressed in hindgut Hindgut allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Cytoplasmic foci Expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Distal tip region-TE Not Expressed in distal tip region -TE Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Individualizing Spermatids - TE Waste Bag - TE Cytoplasmic in individualizing spermatids - TE Cyst cells -TE Tail cyst cell - TE Retracting/Coiling Tail Cyst Cell - TE Retracting/Coiling Tail Cyst Cell Cytoplasm - TE Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed High - TE Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Expression level-SV Expressed High-SV Cell type - SV Cytoplasmic-SV Membrane enriched - SV Cytoplasmic Foci - SV Ejaculatory Duct Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Cytoplasmic foci-SC Main cells -AG Cytoplasmic-MC |
Comments
| All nuclei. Strong in the Amnioserosa. |
| Enriched in the amnioserosa, the central nervous system, the ventral midline, and maybe the tracheal pits. |
| Anterior to posterior gradient Subnuclear localization. |
| The probe is low, but good. Nice insitu data!!! |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Ectoderm Dorsal vessel Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Nuclear in CNS Nuclear in amnioserosa Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body Expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Particular proventriculus area Expressed in pharynx Expressed in midgut Subcellular localization in midgut Midgut - Perinuclear Midgut - Half moon perinuclear midgut Midgut - Nuclear Midgut - Subnuclear Nascent transcript in midgut Expressed in hindgut Subcellular localization in hindgut Nascent transcript in hindgut Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs particular pattern Leg discs few cells - dots Leg discs - outside Expressed in eye disc Eye disc nuclear localization Eye disc subnuclear localization Eye disc few cells - dots Eye disc apical Eye disc - center round cells Expressed in antennal disc Antennal disc few cells - dots Antennal disc - center round cells |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area Ventral nerve cord - nuclear localization Ventral nerve cord - subnuclear localization Localized to axons Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Perinuclear Salivary gland - nuclear dots Nascent transcript in salivary gland Expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Perinuclear localization Perinuclear localization in particular nuclei Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Subcellular localization in lymph glands Lymph Glands - Nuclear localization Lymph Glands - Subnuclear localization Lymph Glands - Cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Individualizing Spermatids - TE Waste Bag - TE Cystic bulge - TE Pigment cells -TE Nuclear in pigment cells -TE Nuclear foci in pigment cells - TE Cyst cells -TE Tail cyst cell - TE Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Nuclear - TE Nuclear foci in elongating tail cyst cell - TE Head cyst cell - TE Nuclear - Head Cyst cell Cyst cell nuclei - TE Subcellularly localized - TE Nuclear Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Germline stem cell Cytoplasmic in germline stem cells - TE Early cyst cell Cytoplasmic in early cyst cell Clusters in early cyst cell-TE Cyst stem cell Cytoplasmic in cyst stem cell Cytoplasmic Seminal Vesicle Cell type - SV Cytoplasmic-SV Membrane enriched - SV Cytoplasmic Foci - SV Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Expression level-SV Expressed Low - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED ECM like pattern-ED Anterior ejaculatory duct papilla-ED Cytoplasmic in anterior ejaculatory papilla-ED Expression level-ED Expressed Medium - ED Distal Enrichment - ED Cell type - ED Cytoplasmic-ED Membrane enriched - ED Cytoplasmic Foci - ED Apical Enrichment-ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Main cells -AG Cytoplasmic-MC Cytoplasmic Foci - AG |
Comments
| Basal part of nuclei. Very interesting. |
| Nuclear, subnuclear. |
| Good probe on gel. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
Probe CR43314-RA Links: Permalink
Genes: CR43314
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Central band of nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Pole cell exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Anterior blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Expression in API Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Posterior spiracles Specific posterior spiracle pattern PNS Midgut Midgut subset Head Head epidermis Clypeo-labral bud Anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in midgut nuclei Nuclear in ectoderm Foci Small Foci Small Foci in PNS Small Foci in ectoderm Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
| -images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Dorsal blastoderm nuclei Weak expression (>41ms exp) |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Dorsal blastoderm nuclei Weak expression (>41ms exp) Localized Embryonic localization patterns Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Embryonic localization patterns Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Posterior spiracles Specific posterior spiracle pattern PNS Oenocytes Midgut Hindgut Specific hindgut area or cells Head CNS Ventral nerve cord Brain Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in other cells Nuclear in ectoderm Nuclear in CNS Nuclear in amnioserosa Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Transiently widespread Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in API Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in API Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Tracheal system Tracheal pits Pharynx Head Ectoderm Blastoderm nuclei Atrium Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in tracheal nuceli Nuclear in pharynx Nuclear in other cells Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| In many nuclei there appear to be several nuclear foci in addition to sites of nascent transcript synthesis. |
| RNA seems to accumulate around most yolk nuclei and expressing blastoderm nuclei. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in amnioserosa |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Midgut Midgut subset Hemocytes Hemocytes specific area Head Fat body Fat body- specific region Fat body polarized Ectoderm Polarized Ectoderm Blastoderm nuclei Amnioserosa Amnioserosa edges Localized Subcellular localization patterns Perinuclear localization Perinuclear around hemocytes Perinuclear around fat body nuclei Perinuclear 'rings' Nuclei-associated localization Nuclear in hemocytes Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus Foci Small Foci Small Foci in amnioserosa Cell junction associated |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Anterior and posterior midgut. |
| Good probe on gel. All over low, with some small foci... low but clear. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Yolk nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Anterior blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Hemocytes Head Ectoderm CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in hemocytes Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Dots inside the nuclei. |
| Tracheal pits, yolk nuclei, some anterior zygotic expression, anterior midgut... Very dynamic and interesting. |
| Nuclear localization. |
| Low probe on gel' BcDNA:RE43210 |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cell division apparatus |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Gap-gene pattern Medium expression (21-40ms exp) Localized Subcellular localization patterns Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Cell division apparatus Apical localization Apical enrichment Diffuse apical localization Apical clusters Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Expression in ectoderm Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Apical localization Apical enrichment Diffuse apical localization Apical clusters Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in ectoderm Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Other subcellular pattern Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cell junction associated Apical localization Apical enrichment Germ cell localization Germ cell enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Segmented pattern Salivary glands Midgut Midgut subset Ectoderm Polarized Ectoderm Dorsal vessel CNS Ventral nerve cord Polarized VNC Perinuclear VNC Blastoderm nuclei Amnioserosa Amnioserosa edges Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around midgut nuclei Perinuclear around ectoderm nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in other cells Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Comments
| Good probe. Very nice insitu patterns! |
| Data from RE10437 probe. |
| Intra-nuclear in balstoderm and yolk nuclei. |
| Intranuclear localization. Amnioserosa specific pattern. etc. Salivary glands and tracheal lumen, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Pharynx Muscles-embryo Muscles - subregion Midgut Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in pharynx Nuclear in muscle cells nuclei Nuclear in ectoderm Accumulation nuclear subregion Foci Bigger Foci |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in hindgut Hindgut allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Wing disc few cells - dots Expressed in leg discs Leg discs few cells - dots Leg discs allover Expressed in eye disc Eye disc few cells - dots Eye disc allover Expressed in antennal disc Antennal disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Borderline between 'not expressed' and 'low expression' |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Localized to larval muscle band Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Different probes, different data. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Polarized tracheal localization Segmented pattern Salivary glands Salivary glands - specific region Proventriculus Posterior spiracles Specific posterior spiracle pattern Pharynx Hindgut Specific hindgut area or cells Head Foregut Esophagus Ectoderm Polarized Ectoderm CNS Ventral nerve cord Clypeo-labral bud Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around tracheal nuclei Perinuclear around proventriculus nuclei Perinuclear around esophagus nuclei Nuclei-associated localization Nuclear in pharynx Nuclear in other cells Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus Cell junction associated Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules few foci Malpighian tubules allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes allover Borderline between 'not expressed' and 'low expression' |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Expressed in salivary glands Salivary glands allover Borderline between 'not expressed' and 'low expression' |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Maybe some 'foci'. |
| Nuclear - posterior spiracles Salivary glands, probably non-specific. |
| Lowish probe on gel. Good in situ! |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Pole cell localization Cytoplasmic foci Apical localization Apical enrichment Discrete apical foci Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in ectoderm Expression in API Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Sensory system Segmented pattern Salivary glands PNS Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Ectoderm CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in tracheal nuceli Nuclear in ectoderm Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus Foci Small Foci Small Foci in PNS Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Nuclear localization and other patterns. |
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Segment polarity pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Expression in API Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in mesoderm Expression in ectoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Accumulation in subset nuclei Embryonic localization patterns Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Proventriculus Pharynx Hindgut Specific hindgut area or cells Head Head epidermis Esophagus Ectoderm Clypeo-labral bud Localized Subcellular localization patterns Nuclei-associated localization Nuclear in proventriculus Nuclear in pharynx Nuclear in ectoderm Foci Small Foci Small foci in Proventriculus Small Foci in Pharynx Small Foci in hindgut Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Ectoderm Anal pad Ring around anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in ectoderm Foci Small Foci Small Foci in PNS Small Foci in ectoderm Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RS isoform transcript (this particular gene has 60 recorded isoforms on FlyBase - further optimization of transcript probe targeting and possibly more probe sets should be considered if a stronger signal is desired) |
| -weak but highly dynamic PNS expression patterns, possibly can be better captured by increasing the probe sets |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei All cortical nuclei Strong expression (1-20ms exp) |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Local terms for zygotic transcripts Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Local terms for zygotic transcripts Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Local terms for zygotic transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Head CNS Ventral nerve cord Nuclear - VNC Brain Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in other cells Nuclear in ectoderm Nuclear in CNS Nuclear in amnioserosa Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus perinuclear Proventriculus perinuclear - few cells Expressed in midgut Subcellular localization in midgut Midgut - Nuclear Midgut - Subnuclear Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - Nuclear in Malpighian tubules - Subnuclear in Expressed in hindgut Subcellular localization in hindgut Nuclear in hindgut Hindgut - subnuclear localization Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca - nuclear localization Gastric caeca - subnuclear localization Nascent transcript in gastric caeca Expressed in esophagus |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - nuclear localization Ventral nerve cord - subnuclear localization Expressed in brain lobes Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - nuclear dots Nascent transcript in salivary gland |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Specific testis expression localization Larval testis foci |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva |
| tracheal system |
|
3rd Instar Larval Tissues Tracheal System Expressed in tracheal tubes |
| lymph glands and ring glands |
|
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Testicular duct-TE Nuclear in Testicular Duct - TE Terminal Epithelium - TE Nuclear in terminal epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatogonium-TE Nuclear in spermatogonium-TE Spermatocytes -TE Nuclear in Spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Individualizing Spermatids - TE Waste Bag - TE Cytoplasmic in individualizing spermatids - TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Pigment cells -TE Nuclear in pigment cells -TE Muscle cells - TE Nuclear in muscle cells -TE Cytoplasmic in muscle cells - TE Cyst cells -TE Tail cyst cell - TE Individualizing Tail Cyst Cell - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Nuclear Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Germline stem cell Cytoplasmic in germline stem cells - TE Early cyst cell Cytoplasmic in early cyst cell Clusters in early cyst cell-TE Cyst stem cell Cytoplasmic in cyst stem cell Lower expression in tip region Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Expression level-SV Expressed Medium - SV Cell type - SV Nuclear-SV Cytoplasmic-SV Perinuclear - SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Expression level-ED Expressed Medium - ED Cell type - ED Nuclear-ED Cytoplasmic-ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Diffuse foci-AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Nuclear-SC Nuclear Foci-SC Cytoplasmic-SC Multivesicular bodies - SC Main cells -AG Nuclear- MC Nuclear foci-mc Cytoplasmic-MC Cytoplasmic Foci - AG |
Comments
| Nuclear |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic PNS Head Ectoderm CNS Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in other cells Nuclear in ectoderm Nuclear in CNS Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Allover with small foci. |
| Sub-nuclear. |
| Good probe on gel. Allover with small foci. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Segmented pattern Posterior spiracles Head Head epidermis Ectoderm Polarized Ectoderm Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Foci Small Foci Small Foci in ectoderm Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 6 probe sets targeting the RD isoform transcript (previously tested with 3 and was not detected) |
| -probes are from two different plates (3 sets from each plate); SH-HCR-Plate-1 and SH-HCR-Plate-4 |
| -this gene is very weakly expressed and more probe sets (if using HCR-FISH) should be considered for future retests |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Yolk plasm localization Yolk cortex localization Nuclei-associated localization Intranuclear accumulation Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Discrete apical foci Degraded Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in mesoderm Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Optic lobes primordium Neuroblasts Maxillary complex Head Head epidermis Ectoderm Dorsal vessel CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in ectoderm Nuclear in CNS Foci Small Foci Small Foci in head Small Foci in ectoderm Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -very diverse and neat patterns at all stages |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Medium expression (21-40ms exp) Localized Subcellular localization patterns Apical localization Apical enrichment Apical clusters |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Apical localization Apical enrichment Apical Localization Neuroblasts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern Salivary glands Salivary glands lumen Ring gland Proventriculus PNS Pharynx Midgut Midgut subset Hindgut Specific hindgut area or cells Head Foregut Ectoderm Polarized Ectoderm CNS Ventral nerve cord Perinuclear VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around ectoderm nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in ectoderm Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Neuroblast, interesting. |
| Nice stripes! |
| Lowish, good probe. Very nice insitu! |