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Result Summary
| Number of genes | 9 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Medium expression (21-40ms exp) Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Localized Embryonic localization patterns Ventral localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands - specific region Ring gland Muscles-embryo Visceral mesoderm Hindgut Head CNS Ventral nerve cord Localized Subcellular localization patterns Basal enrichment Basal in salivary glands Basal in hindgut |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system Borderline between 'not expressed' and 'low expression' |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cell division apparatus Other cell division pattern Chromatin associated metaphase |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Nuclei-associated localization Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Cell division apparatus Other cell division pattern Centrosomal localization Apical localization Apical enrichment Discrete apical foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Posterior spiracles Midgut Ectoderm Localized Subcellular localization patterns Membrane associated Basal enrichment Basal in salivary glands Basal in midgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel In situ should be repeated for confirmation. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Perinuclear around yolk nuclei Tracheal system Tracheal lumen Salivary glands Salivary glands - specific region Posterior spiracles Hemocytes Ectoderm CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around hemocytes Membrane associated Foci Small Foci Small Foci in hemocytes Basal enrichment Basal in salivary glands Basal (outside) in CNS Basal (outside) in brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
|
| stage 4-5 |
Stage 4-5 Non-Expressed Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Salivary glands Salivary glands lumen Salivary glands - specific region Blastoderm nuclei Localized Subcellular localization patterns Basal enrichment Basal in salivary glands |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Part of the salivary glands expression may be non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Yolk plasm localization Yolk plasm enrichment Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Salivary glands Salivary glands lumen Salivary glands - specific region Hindgut Specific hindgut area or cells Fat body CNS Ventral nerve cord Axons Atrium Localized Subcellular localization patterns Membrane associated Foci Small Foci Small Foci in hindgut Cell extensions or 'podia' Basal enrichment Basal in salivary glands Basal in many tissues Basal (outside) in CNS Apical enrichment Apical enriched hindgut |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Fat body - Few and small foci Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Proventriculus allover Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Malpighian tubules allover Expressed in gastric caeca Nascent transcript in gastric caeca Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc allover Expressed in eye disc Eye disc allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - allover Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Nascent transcript in salivary gland Expressed in salivary glands Salivary glands allover |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Nascent transcript in ring gland Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Comments
| Basal and membrane looking. Tracheal lumen may be non-specific. |
| Good, strong probe. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Yolk cortex localization Yolk cortex enrichment Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Maternal Localized Yolk plasm localization Yolk plasm enrichment Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Exclusionary patterns Apical exclusion Basal localization Basal enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Maternal Localized Yolk plasm localization Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Basal localization Basal enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Salivary glands Salivary glands lumen Salivary glands - specific region Muscles-embryo Visceral mesoderm Midgut Ectoderm CNS Ventral nerve cord Localized Subcellular localization patterns Membrane associated Basal enrichment Basal in salivary glands Basal in many tissues Basal (outside) in CNS |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Proventriculus allover Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Hindgut allover Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Wing disc few cells - dots Expressed in leg discs Leg discs few cells - dots Leg discs allover Expressed in eye disc Eye disc photo-receptors Eye disc few cells - dots Expressed in antennal disc Antennal disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes allover |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - nuclear dots Nascent transcript in salivary gland Expressed in salivary glands Salivary glands allover |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Nascent transcript in ring gland Expressed in ring gland Ring gland allover Lymph Glands Expressed in lymph glands Lymph glands all over |
Adult
| Area | Images | Terms |
|---|
Comments
| Basal localization |
| Good probe. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Embryonic localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Yolk cortex exclusion Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Perinuclear around yolk nuclei Inside Yolk nuclei Tracheal system Specific tracheal branches Segmented pattern Salivary glands Salivary glands lumen Salivary glands - specific region PNS Midgut Malpighian tubules Hindgut Ectoderm CNS Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear around tracheal nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in midgut Small Foci in Malpighian tubules Small Foci in hindgut Small Foci in ectoderm Basal enrichment Basal in salivary glands |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Perinuclear localization Half moon perinuclear Cytoplasmic foci-fat body Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Expressed in midgut Subcellular localization in midgut Midgut - Perinuclear Midgut - Half moon perinuclear midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - Perinuclear Malpighian tubules - Half moon perinuclear Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Hindgut allover Expressed in gastric caeca Nascent transcript in gastric caeca |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Wing disc few cells - dots Expressed in leg discs Leg discs few cells - dots Leg discs allover Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc few cells - dots Expressed in antennal disc Antennal disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Expressed in brain lobes Optic lobe Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Perinuclear Salivary gland - Half moon perinuclear Nascent transcript in salivary gland Expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Perinuclear Ring gland - Half moon perinuclear Ring gland - Cytoplasmic foci Nascent transcript in ring gland Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| At stage 5 there is some apical, and some pole cell expression (few dots). |
| Experiment done 3x. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Random blastoderm nuclei Expression in API Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Apical exclusion Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in amnioserosa Anterior blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands lumen Salivary glands - specific region Proventriculus Posterior spiracles Midgut Midgut subset Midgut ICPs Hindgut Specific hindgut area or cells Head Foregut Fat body Ectoderm CNS Ventral nerve cord Polarized VNC Perinuclear VNC Polarized CNS Brain Atrium Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Foci Small Foci Small Foci in ectoderm Basal enrichment Basal in salivary glands Basal in midgut Basal in hindgut Basal (outside) in CNS Apical enrichment Apical in other tissues |
Larva
| Area | Images | Terms |
|---|---|---|
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Comments
| Good probe on gel. Many photos taken by my son: Adam Wilk - 9 years old (with supervision)--Ronit |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Basal localization Basal enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Expression in API Expression in amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Basal localization Basal enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Salivary glands Salivary glands lumen Salivary glands - specific region Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Muscles - subregion Midgut Midgut subset Hindgut Specific hindgut area or cells Head CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in other tissues Small Foci in muscle cells Bigger Foci Basal enrichment Basal in salivary glands Basal in midgut Basal in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Basal in hindgut and midgut. Specific region of muscle cells. Specific visceral mesoderm pattern' Tracheal lumen, probably non-specific |
| I see mainly 'one dot per nuclei' in the amnioserosa. This gene is on 3L, so zygotic expression should have 'two dots'. Therefore, I annotated it as 'nuclear subregion' and 'intra-nuclear accumulation'. |