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| Number of genes | 49 | |
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| -sample background seems to high and does not match current data - candidate for retest (2025) |
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| Enriched at the Instersticial cell progenitors (midgut). |
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| Salivary glands, probably non-specific. |
| The probe looks good on gel The photos are from a combination of 2 experiments. One had a brighter probe than the other, which explains why some photos are brighter than others. |
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| Good probe on gel. Allover with small foci. |
| Salivary glands and tracheal lumen, probably non-specific. |
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| Salivary glands, probably non-specific. |
| Nice expression and localization. |
| Low probe, good but not bright. Small foci. |
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| Re-annotated |
| Partly nascent, partly nuclear subregion |
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| Good probe. |
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| Good probe. |
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| Re-annotated |
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| -performed via HCR-FISH using 2 probe sets targeting the RA isoform transcript |
| -this non-coding gene is an extremely highly expressed lncRNA in male testes according to scRNA-seq data, and exhibits a strong nuclear pattern in in embryo yolk, pole cell, and random blastoderm cells |
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| The probe may be recognizing 16 transcripts - Including flamenco. The probe may recognize some other areas in the genome that contain a highly homologous sequence. |
| Amazing patterns! |
| Very interesting patterns in adult testes and associated tissues. |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -previously reported in another study unrelated to us to show maternal RNA expression (via TSA-FISH) but this was not detected in our attempt using HCR-FISH |
| -appears to be ubiquitously expressed in the nucleus of all blastoderm derived tissues, and expressed around the yolk nuclei |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -diverse ectoderm derived structures such as the salivary gland (and the salivary primordium), amnioserosa, proventriculus, tracheal pits, and epidermis |
| -prior reported expression of hemocytes were not visible in this experiment |
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| Probe looks good on gel. |
| Specific localization around the yolk nuclei... |
| Specific localization around the yolk nuclei... Very interesting. |
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| Good probe on gel. |
| Tracheal lumen, probably non-specific. |
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| Good probe, good plate |
| The probe recognizes the transposon and also flamenco and maybe some other areas in the genome that contain a highly homologous sequence. |
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| Salivary glands and tracheal lumen, probably non-specific. |
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| Low expression (144ms) |
| Head, yolk nuclei |
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| In many nuclei there appear to be several nuclear foci in addition to sites of nascent transcript synthesis. |
| RNA seems to accumulate around most yolk nuclei and expressing blastoderm nuclei. |
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| Good probe on gel. |
| Maybe some expression, low. |
| Yolk nuclei podia. |
| Nice expression in the yolk nuclei, peri-nuclear and cytoplasmic foci. |
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| Partly nascent, partly nuclear subregion. |
| Good probe on gel. |
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| Dots inside the nuclei. |
| Tracheal pits, yolk nuclei, some anterior zygotic expression, anterior midgut... Very dynamic and interesting. |
| Nuclear localization. |
| Low probe on gel' BcDNA:RE43210 |
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| Good probe on gel |
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| Good probe on gel. Small foci. Low expression. |
| Low expression. |
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| 84 ms I think it is all zygotic, nothing sub-cellular (?). I wish we had 40x images. |
| 84 ms |
| 84 ms |
| 84 ms Re-annotated by Ronit- 2014. |
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| -performed via HCR-FISH using 3 probe sets targeting common exons |
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| -U2 us encoded at at least 6 different loci which this probe tries to target -U2: 34ABa 2L CR31850, U2: 34ABb 2L CR31854, U2: 34ABc 2L CR33788, U2: 38ABb 2L CR32878, U2: 38ABa 2L CR32882, and U2: 14B X CR32913 |
| -testing via HCR-FISH using a single probe set common to all listed loci |
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| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
| -expression in primordial adult eye is not observed in this experiment but was reported by BDGP |
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| Nuclear expression. Dots inside all yolk nuclei. |
| Salivary glands, may be non-specific. |
| Low probe on gel... good insitu |
| It is more 'cytoplasmic foci around pole cells'. |
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| Nuclear in tracheal pits. Anterior and posterior midgut. YN. |
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