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Result Summary
| Number of genes | 21 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm Localized Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Malpighian tubules Head Head epidermis Ectoderm Anal pad |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Pole cell localization Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Central band of nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Posterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Maternal Localized Subcellular localization patterns Posterior localization RNA islands Pole plasm Pole buds Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Maternal Localized Pole cell localization Pole cell enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Maternal Localized Germ cell localization Germ cell enrichment Embryonic localization patterns Posterior localization |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in genital disc Expressed in eye disc Morphogenetic furrow Eye disc few cells - dots Eye disc apical Eye disc allover Expressed in antennal disc Antennal disc few cells - dots Antennal disc - localized outside |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Mushroom body |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - apical Salivary gland - lumen Expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
Probe CG31131-ex Links: Permalink
Genes: CG31131
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Posterior localization Embryonic localization patterns Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Weak expression (>41ms exp) Ubiquitous Localized Embryonic localization patterns Posterior localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Ectoderm Weak expression (>41ms exp) Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
| -modENCODE RNAseq data on FlyBase shows low/no expression, however, we observed strong expression patterns throughout development |
Probe CG42239-RA Links: Permalink
Genes: CG42239
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Yolk cortex localization Yolk cortex enrichment Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Ubiquitous Localized Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Exclusionary patterns Yolk plasm exclusion Cytoplasmic foci Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Anterior blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Posterior localization Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Non-Expressed |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -becomes very hard to detect after early germband elongation and is rapidly degraded, it may still be weakly ubiquitous in later stages but expression levels border between expressed and non-expressed |
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Maternal Localized Yolk cortex localization Subcellular localization patterns Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Embryonic localization patterns Posterior localization Degraded Degraded partially stage 8 Degradation maternal transcripts |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Posterior enriched |
Probe CR43617-RA Links: Permalink
Genes: CR43617
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Single stripe Segmented pattern Gap-gene pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei All blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Posterior localization Cytoplasmic foci Basal localization Basal enrichment Apical localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Gap-gene pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Exclusionary patterns Apical exclusion Cytoplasmic foci Basal localization Basal enrichment Apical localization Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Degraded Degraded partially stage 7 |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Gap-gene pattern Dorsal blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization Posterior localization Dorsal localization Degraded Degraded partially stage 8 Degraded completely stage 9 |
| stage 10-17 |
Stage 10-17 Non-Expressed |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -posterior single stripe; expression is reported to be similar to the lncRNA "iab4" (CR31271) |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Embryonic localization patterns Posterior localization Anterior localization Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Posterior spiracles Pharynx Hindgut Hemocytes Head Fat body Ectoderm Dorsal vessel Localized Subcellular localization patterns Perinuclear localization Perinuclear around hemocytes |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| All over |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Posterior localization Embryonic localization patterns Exclusionary patterns Pole cell exclusion Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Posterior localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Segmented pattern Midgut Midgut subset Head Ectoderm Polarized Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around tracheal nuclei Perinuclear around ectoderm nuclei |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Possible peripodial membrane expression Expressed in leg discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva Specific ovary expression localization Ovary nuclear 'dot' Ovary foci Expressed in Ovary |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Sub and peri nuclear with apical enrichment. Anterior cells only. Very specific and interesting. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole plasm Degraded Degraded partially stage 3 |
| stage 4-5 |
|
Stage 4-5 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Posterior localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Degraded Degraded partially stage 5 |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Localized Embryonic localization patterns Posterior localization Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Salivary glands Midgut Hindgut Head CNS Blastoderm nuclei Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Anterior midgut, stronger at the posterior midgut. |
| Probe looks good on gel |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Embryonic localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Posterior spiracles Muscles-embryo Muscles - subregion Maxillary complex Head Head epidermis Ectoderm Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -There is no entry for this gene on BDGP, and modENCODE RNAseq data on FlyBase indicates that it is not expressed in the embryo. However, we observed obvious patterns during embryogenesis. Additionally, FlyBase does not have any in-depth characterizations of this gene. |
| -Osi21 does not overlap with any other genes, ruling out the possibility of the targeting of alternative transcripts. BLAST also confirms the specificity of our probes to Osi21. Additionally, no other genes for which probes were designed for on this plate show patterns such as those observed here, ruling out the possibility of cross-contamination during probe preparation. |
| -This may be a result of Osi21's poor characterization or incorrect RNAseq data |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Posterior localization Embryonic localization patterns Anterior localization Degraded Degraded partially stage 6 |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Localized Embryonic localization patterns Posterior localization Germ band localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Midgut subset Head Head epidermis Ectoderm Dorsal vessel CNS Ventral nerve cord Foci - VNC Brain Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Ventral localization Posterior localization Dorsal localization Anterior localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Head Head epidermis Ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Single stripe Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Single stripe Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Basal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Single stripe Segmented pattern Expression in mesoderm Expression in ectoderm Expression in amnioserosa Dorsal blastoderm nuclei Medium expression (21-40ms exp) Localized Embryonic localization patterns Posterior localization Germ band localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Muscles - subregion Hindgut Ectoderm CNS Ventral nerve cord Strong expression (1-20ms exp) Localized Subcellular localization patterns Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -possesses a striking segmented pattern in the epidermis, mesoderm, and VNC; reports of expression in the cardiac primordium and dorsal vessel were not clearly observed and could possibly be amnioserosa expression |
| -a classic homeobox gene |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Localized Subcellular localization patterns Ventral localization Basal localization Basal enrichment Degraded Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Basal localization Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Posterior localization Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Neuroblasts Polarized Neuroblasts Head Head epidermis Ectoderm CNS Ventral nerve cord Polarized VNC Perinuclear VNC Brain Localized Subcellular localization patterns Foci Small Foci Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -neat marker for neural precursor formation, appears to not be expressed past stage 13-14 except for 4 small points near the dorsal-posterior region of the ectoderm (possibly tip cells of the Malpighian tubules); clear stomatogastric nervous system primordium expression |
| -images are from two separate experiments under identical conditions for better coverage |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Expression in API Anterior blastoderm nuclei Ubiquitous Localized Embryonic localization patterns Ventral localization Posterior localization Germ band localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Weak expression (>41ms exp) Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole plasm Embryonic localization patterns Degraded Degraded partially stage 3 Degradation maternal transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns Degraded Degraded partially stage 7 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Embryonic localization patterns Posterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Midgut Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Posterior localization Pole cell localization Pole cell enrichment Exclusionary patterns Apical exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Germ cell localization Germ cell enrichment Embryonic localization patterns Posterior localization Degraded Degraded completely stage 8 Degradation maternal transcripts |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Maternal RNA is posteriorly localized in yolk plasm and then in pole cells. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole plasm Pole buds Nuclei associated localization Perinuclear localization Cytoplasmic foci Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Maternal Localized Germ cell localization Germ cell enrichment Embryonic localization patterns Posterior localization Degraded Degraded partially stage 9 Degraded partially stage 8 Degradation maternal transcripts |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel In unfertilized eggs, and 2 nuclei embryos: I see osk very strongly expressed posteriorly (as expected) AND anterior expression as well (see 2 nuclei anterior 40x photo). The RNA probe shows anterior 'structures' (interesting and probably novel). |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization RNA islands Pole plasm Pole buds Other subcellular pattern Nuclei associated localization Perinuclear localization Perinuclear around pole cell nuclei Cell division apparatus Other cell division pattern Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Pole cell nuclei Subset pole cell nuclei Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cell division apparatus Other cell division pattern Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei Subset pole cell nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cell division apparatus Other cell division pattern Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Other subcellular pattern Nuclei-associated localization Cell division apparatus Germ cell localization Germ cell enrichment Embryonic localization patterns Posterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Germ cell nuclei Blastoderm nuclei Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Again, perinuclear pole cell asymmetric expression. |
| Pole cell asymmetric expression. I see small 'dots' around the pole cells, stronger in one 1/2 of the pole nuclei. |
| Good probe on gel |
| Posterior localization, even in unfertilized eggs. RNA accumulates asymmetrically around the pole buds with a 'fiber-like' texture. When nuclei divide, the RNA will stay in the nuclei that are closer to the posterior end. Very interesting! |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Yolk plasm localization Yolk cortex localization Nuclei-associated localization Intranuclear accumulation Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Discrete apical foci Degraded Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in mesoderm Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Optic lobes primordium Neuroblasts Maxillary complex Head Head epidermis Ectoderm Dorsal vessel CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in ectoderm Nuclear in CNS Foci Small Foci Small Foci in head Small Foci in ectoderm Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -very diverse and neat patterns at all stages |