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| Number of genes | 44 | |
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| Re-annotated |
| Amnioserosa |
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| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
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| Maternal mRNA is posteriorly localized in pole plasm and forms RNA islands around pole cell nuclei. Localization persists in pole/germ cells until 6-7. |
| Zygotic mRNA expressed from yolk nuclei accumulates in clouds surrounding the yolk nuclei. The RNA also seems to accumulate in or tightly around a few nuclei that are entering the blastoderm layer from the yolk. |
| -Missing late stages - needs to be retested for full coverage of expression patterns (2024) |
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| Salivary glands, probably non-specific. |
| Nice expression and localization. |
| Low probe, good but not bright. Small foci. |
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| Re-annotated |
| Partly nascent, partly nuclear subregion |
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| 69 ms |
| 69 ms |
| 40 ms |
| 69 ms. Reannotaded... it was as 'no expressed'! |
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| Re-annotated |
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| -performed via HCR-FISH using 2 probe sets targeting the RA isoform transcript |
| -this non-coding gene is an extremely highly expressed lncRNA in male testes according to scRNA-seq data, and exhibits a strong nuclear pattern in in embryo yolk, pole cell, and random blastoderm cells |
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| The probe may be recognizing two transcripts: CR45030 and CG11873 |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -seems to agree with modENCODE RNA-seq data |
| -interesting yolk and mesoderm patterns in trunk and head |
| The probe may be recognizing 16 transcripts - Including flamenco. The probe may recognize some other areas in the genome that contain a highly homologous sequence. |
| Amazing patterns! |
| Very interesting patterns in adult testes and associated tissues. |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -previously reported in another study unrelated to us to show maternal RNA expression (via TSA-FISH) but this was not detected in our attempt using HCR-FISH |
| -appears to be ubiquitously expressed in the nucleus of all blastoderm derived tissues, and expressed around the yolk nuclei |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -diverse ectoderm derived structures such as the salivary gland (and the salivary primordium), amnioserosa, proventriculus, tracheal pits, and epidermis |
| -prior reported expression of hemocytes were not visible in this experiment |
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| Re-annotated. |
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| In many nuclei there appear to be several nuclear foci in addition to sites of nascent transcript synthesis. |
| RNA seems to accumulate around most yolk nuclei and expressing blastoderm nuclei. |
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| 15 ms |
| 51 ms |
| 25 ms |
| 15 ms No zygotic foci, perhaps due to masking by signal from cytoplasmic RNA. |
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| Dots inside the nuclei. |
| Tracheal pits, yolk nuclei, some anterior zygotic expression, anterior midgut... Very dynamic and interesting. |
| Nuclear localization. |
| Low probe on gel' BcDNA:RE43210 |
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| Good probe on gel. Small foci. Low expression. |
| Low expression. |
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| 84 ms I think it is all zygotic, nothing sub-cellular (?). I wish we had 40x images. |
| 84 ms |
| 84 ms |
| 84 ms Re-annotated by Ronit- 2014. |
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| -performed via HCR-FISH using 3 probe sets targeting common exons |
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| -U2 us encoded at at least 6 different loci which this probe tries to target -U2: 34ABa 2L CR31850, U2: 34ABb 2L CR31854, U2: 34ABc 2L CR33788, U2: 38ABb 2L CR32878, U2: 38ABa 2L CR32882, and U2: 14B X CR32913 |
| -testing via HCR-FISH using a single probe set common to all listed loci |
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| -also known as scaRNA:MeU5-C46 |
| -tested using HCR-FISH using a 5 probe sets |
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| Male reproductive tissues |
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| Good probe on gel. Nice insitu. |
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| Very interesting pattern. |
| Re-annotated |
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