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Number of genes | 41 | |
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Re-annotated |
Amnioserosa |
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-performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
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Maternal mRNA is posteriorly localized in pole plasm and forms RNA islands around pole cell nuclei. Localization persists in pole/germ cells until 6-7. |
Zygotic mRNA expressed from yolk nuclei accumulates in clouds surrounding the yolk nuclei. The RNA also seems to accumulate in or tightly around a few nuclei that are entering the blastoderm layer from the yolk. |
-Missing late stages - needs to be retested for full coverage of expression patterns (2024) |
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Salivary glands, probably non-specific. |
Nice expression and localization. |
Low probe, good but not bright. Small foci. |
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Re-annotated |
Partly nascent, partly nuclear subregion |
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69 ms |
69 ms |
40 ms |
69 ms. Reannotaded... it was as 'no expressed'! |
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Re-annotated |
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-performed via HCR-FISH using 2 probe sets targeting the RA isoform transcript |
-this non-coding gene is an extremely highly expressed lncRNA in male testes according to scRNA-seq data, and exhibits a strong nuclear pattern in in embryo yolk, pole cell, and random blastoderm cells |
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The probe may be recognizing two transcripts: CR45030 and CG11873 |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-seems to agree with modENCODE RNA-seq data |
-interesting yolk and mesoderm patterns in trunk and head |
The probe may be recognizing 16 transcripts - Including flamenco. The probe may recognize some other areas in the genome that contain a highly homologous sequence. |
Amazing patterns! |
Very interesting patterns in adult testes and associated tissues. |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-previously reported in another study unrelated to us to show maternal RNA expression (via TSA-FISH) but this was not detected in our attempt using HCR-FISH |
-appears to be ubiquitously expressed in the nucleus of all blastoderm derived tissues, and expressed around the yolk nuclei |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-diverse ectoderm derived structures such as the salivary gland (and the salivary primordium), amnioserosa, proventriculus, tracheal pits, and epidermis |
-prior reported expression of hemocytes were not visible in this experiment |
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Re-annotated. |
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In many nuclei there appear to be several nuclear foci in addition to sites of nascent transcript synthesis. |
RNA seems to accumulate around most yolk nuclei and expressing blastoderm nuclei. |
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15 ms |
51 ms |
25 ms |
15 ms No zygotic foci, perhaps due to masking by signal from cytoplasmic RNA. |
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Dots inside the nuclei. |
Tracheal pits, yolk nuclei, some anterior zygotic expression, anterior midgut... Very dynamic and interesting. |
Nuclear localization. |
Low probe on gel' BcDNA:RE43210 |
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Good probe on gel. Small foci. Low expression. |
Low expression. |
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84 ms I think it is all zygotic, nothing sub-cellular (?). I wish we had 40x images. |
84 ms |
84 ms |
84 ms Re-annotated by Ronit- 2014. |
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Male reproductive tissues |
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Good probe on gel. Nice insitu. |
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Very interesting pattern. |
Re-annotated |
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