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| Number of genes | 36 | |
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| Salivary glands, probably non-specific. |
| The probe looks good on gel The photos are from a combination of 2 experiments. One had a brighter probe than the other, which explains why some photos are brighter than others. |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -appears to be weakly ubiquitously expressed at all stages |
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| Zygotically expressed RNA exhibits clumpy localization in apical cytoplasm. |
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| Low probe on gel Nice in situ! |
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| -performed via HCR-FISH using 2 probe sets targeting the RA isoform transcript |
| -this non-coding gene is an extremely highly expressed lncRNA in male testes according to scRNA-seq data, and exhibits a strong nuclear pattern in in embryo yolk, pole cell, and random blastoderm cells |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
| -images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
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| Home made probe to last exon. Covers all isoforms. |
| Salivary glands and tracheal lumen, probably non-specific. |
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| The wingless probe is on the next row of the same plate... Potential cross-contamination. |
| Good probe on gel. |
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| Good probe on gel. |
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| -performed via HCR-FISH using 3 probe sets targeting the RI isoform transcript; difficult to pick best isoform to design probes to due to vast number of them, hard to say if these probes captured the complete expression profile for this gene |
| -Our previous experiments using TSA and independent experiments by BDGP observed maternal expression in stages 1-3 and stages 4-5, this was not observed in this experiment |
| -Interesting early mesoderm marker |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -no longer detected after stages 6-7, probably expressed weakly and ubiquitously at all later stages |
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| Dots inside the nuclei. |
| Tracheal pits, yolk nuclei, some anterior zygotic expression, anterior midgut... Very dynamic and interesting. |
| Nuclear localization. |
| Low probe on gel' BcDNA:RE43210 |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -strong posterior midgut primordium expression |
| -possible expression in sensory organs in head and posterior epidermis |
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| Ring gland (I think) |
| Good probe on gel. |
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| Good probe on gel ______ Posterior 'foci'... interesting. Enriched at the pole plasm. |
| Salivary glands, midgut, hindgut, visceral mesoderm, muscles, etc. |
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| Good probe on gel. |
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| Re-annotated RW |
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| -detected using HCR-FISH protocol (3 probe sets) |
| -probes were designed against dap-RA isoform transcript |
| -really neat expression patterns |
| -images are from two separate experiments under identical conditions for better coverage |
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| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
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| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -neat and diverse expression patterns throughout development |
| -images are from two separate experiments under identical conditions for better coverage |
| Nuclear |
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| -performed via HCR-FISH using 6 probe sets targeting the RA isoform transcript (previously tested with 3 and signals bordered between expressed and non-expressed) |
| -exceptional expression patterning in early epidermis and ring gland |
| -probes are from two different plates (3 sets from each plate); SH-HCR-Plate-1 and SH-HCR-Plate-4 |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -expression seen in the stomatogastric sensory primordium, and the anterior and posterior midgut primordium; forms a complex along with ac (CG3796) so should colocalize in neuronal regions |
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| Good probe on gel. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -very diverse and neat patterns at all stages |
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| Nervous system. Nuclear. |
| Central nervous system. |
| Nuclear. Nervous system. |
| Good probe on gel |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -good marker for the neuroectoderm region of the presumptive ventral nerve cord on the germ band; possible weak but faint epidermal signal detected in later embryos |
| Good probe on gel. |
| Specific neuroblast expression. |
| Some photos are from the 2006 initial screen. More photos added in 2014 and many more terms. |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -neat expression patterns in early embryos and early germ band elongation stages; expression is no longer detectable after stage 9-10 (fits modENCODE RNA-seq data well) |