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Number of genes | 31 | |
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Salivary glands, probably non-specific. |
The probe looks good on gel The photos are from a combination of 2 experiments. One had a brighter probe than the other, which explains why some photos are brighter than others. |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-appears to be weakly ubiquitously expressed at all stages |
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Zygotically expressed RNA exhibits clumpy localization in apical cytoplasm. |
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Low probe on gel Nice in situ! |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
-images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
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Home made probe to last exon. Covers all isoforms. |
Salivary glands and tracheal lumen, probably non-specific. |
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The wingless probe is on the next row of the same plate... Potential cross-contamination. |
Good probe on gel. |
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Good probe on gel. |
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-performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
-no longer detected after stages 6-7, probably expressed weakly and ubiquitously at all later stages |
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Dots inside the nuclei. |
Tracheal pits, yolk nuclei, some anterior zygotic expression, anterior midgut... Very dynamic and interesting. |
Nuclear localization. |
Low probe on gel' BcDNA:RE43210 |
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Ring gland (I think) |
Good probe on gel. |
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Good probe on gel ______ Posterior 'foci'... interesting. Enriched at the pole plasm. |
Salivary glands, midgut, hindgut, visceral mesoderm, muscles, etc. |
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Good probe on gel. |
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Re-annotated RW |
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-detected using HCR-FISH protocol (3 probe sets) |
-probes were designed against dap-RA isoform transcript |
-really neat expression patterns |
-images are from two separate experiments under identical conditions for better coverage |
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-performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
-neat and diverse expression patterns throughout development |
-images are from two separate experiments under identical conditions for better coverage |
Nuclear |
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-performed via HCR-FISH using 6 probe sets targeting the RA isoform transcript (previously tested with 3 and signals bordered between expressed and non-expressed) |
-exceptional expression patterning in early epidermis and ring gland |
-probes are from two different plates (3 sets from each plate); SH-HCR-Plate-1 and SH-HCR-Plate-4 |
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-performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
-expression seen in the stomatogastric sensory primordium, and the anterior and posterior midgut primordium; forms a complex along with ac (CG3796) so should colocalize in neuronal regions |
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Good probe on gel. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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-performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
-very diverse and neat patterns at all stages |
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Nervous system. Nuclear. |
Central nervous system. |
Nuclear. Nervous system. |
Good probe on gel |
Good probe on gel. |
Specific neuroblast expression. |
Some photos are from the 2006 initial screen. More photos added in 2014 and many more terms. |
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-performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
-neat expression patterns in early embryos and early germ band elongation stages; expression is no longer detectable after stage 9-10 (fits modENCODE RNA-seq data well) |