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Number of genes | 40 | |
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The probe looks good on gel. |
It is expressed on the ventral side of the developing embryo, it accumulates in the cytoplasm. |
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Nice probe on gel. |
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Salivary glands, probably non-specific. |
Good probe Low maternal. |
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Good probe on gel. |
Good probe on gel. |
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Good probe on gel. |
Interesting expression |
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Low probe on gel Nice in situ! |
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Low probe on gel... still, good in situ. |
Salivary glands, midgut and other expression. |
Zygotic expression at the anterior and posterior midgut nuclei and other cells. Dynamic expression. |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
-images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-posterior single stripe; expression is reported to be similar to the lncRNA "iab4" (CR31271) |
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-performed via HCR-FISH using 3 probe sets targeting the RC isoform transcript |
-the gene is a lncRNA with quite interesting patterns in the CNS (brain and VNC) |
-seems to be expressed in the anterior transverse furrow, shown in stage 6-7 images |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-interesting 7-stripe pattern observed in the blastoderm/epidermis |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-diverse ectoderm derived structures such as the salivary gland (and the salivary primordium), amnioserosa, proventriculus, tracheal pits, and epidermis |
-prior reported expression of hemocytes were not visible in this experiment |
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Good probe on gel. |
Maybe membranes... need better data. |
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Low probe. |
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Very low probe on gel. |
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Probe on gel a bit low. Good in situ! |
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-performed via HCR-FISH using 3 probe sets targeting the RI isoform transcript; difficult to pick best isoform to design probes to due to vast number of them, hard to say if these probes captured the complete expression profile for this gene |
-Our previous experiments using TSA and independent experiments by BDGP observed maternal expression in stages 1-3 and stages 4-5, this was not observed in this experiment |
-Interesting early mesoderm marker |
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Good probe on gel |
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Nice probe on gel. |
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Salivary glands, probably non-specific. High in yolk. |
Important note: these images are from a home made probe for the Sdc gene. |
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Very small foci. |
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Good probe on gel. |
All over, including pole cells and apical localization... |
Cytoplasmic foci. |
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-performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
-neat marker for neural precursor formation, appears to not be expressed past stage 13-14 except for 4 small points near the dorsal-posterior region of the ectoderm (possibly tip cells of the Malpighian tubules); clear stomatogastric nervous system primordium expression |
-images are from two separate experiments under identical conditions for better coverage |
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-performed via HCR-FISH (3 probe sets) |
-isoform corn-RC transcript was targeted |
-weak expression in the late embryos that appear as small points along the ectoderm of the each segment (trunk and abdominal) located dorsolateral. |
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Good probe on gel. |
Tracheal pits, muscle (cytoplasmic), visceral mesoderm. |
Tracheal pits, posterior midgut, other cells. |
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Good probe on gel. |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-retested recently for late stage coverage (2025); appears to be expressed in a different program in the gnathal elements and sensory regions in late stages |
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-performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
-neat and diverse expression patterns throughout development |
-images are from two separate experiments under identical conditions for better coverage |
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-performed via HCR-FISH using 6 probe sets targeting the RA isoform transcript (previously tested with 3 and signals bordered between expressed and non-expressed) |
-exceptional expression patterning in early epidermis and ring gland |
-probes are from two different plates (3 sets from each plate); SH-HCR-Plate-1 and SH-HCR-Plate-4 |
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-performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
-expression seen in the stomatogastric sensory primordium, and the anterior and posterior midgut primordium; forms a complex along with ac (CG3796) so should colocalize in neuronal regions |
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Good probe on gel. |
Interesting expression and localization patterns. |
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-performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
-very diverse and neat patterns at all stages |
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Salivary glands, probably non-specific Need more images to tell the full story. |
Low probe, nice insitu. |