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| Number of genes | 48 | |
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| Re-annotated |
| Amnioserosa |
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| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
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| Maternal mRNA is posteriorly localized in pole plasm and forms RNA islands around pole cell nuclei. Localization persists in pole/germ cells until 6-7. |
| Zygotic mRNA expressed from yolk nuclei accumulates in clouds surrounding the yolk nuclei. The RNA also seems to accumulate in or tightly around a few nuclei that are entering the blastoderm layer from the yolk. |
| -Missing late stages - needs to be retested for full coverage of expression patterns (2024) |
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| 68 ms |
| 68 ms |
| 68 ms |
| 68 ms |
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| Good probe on gel. |
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| Good probe on gel. |
| Partly nascent, partly sub-nuclear. |
| Maybe Partly nascent, partly sub-nuclear. |
| Salivary glands, probably non-specific. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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| Re-annotated |
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| Good probe on gel. |
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| Re-annotated. |
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| Maternal RNA localizes to pole plasm and remains localized to pole cells and germ cells at later stages. |
| Zygotic RNA accumulates in the periphery of expressing yolk nuclei. |
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| Salivary glands, probably non-specific. |
| Good probe on gel. |
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| Low but good probe. |
| Interesting expression. |
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| Re-annotated |
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| -performed via HCR-FISH using 2 probe sets targeting the RA isoform transcript |
| -this non-coding gene is an extremely highly expressed lncRNA in male testes according to scRNA-seq data, and exhibits a strong nuclear pattern in in embryo yolk, pole cell, and random blastoderm cells |
| The probe may be recognizing 16 transcripts - Including flamenco. The probe may recognize some other areas in the genome that contain a highly homologous sequence. |
| Amazing patterns! |
| Very interesting patterns in adult testes and associated tissues. |
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| Tracheal lumen, probably non-specific. |
| Good probe. |
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| Re-annotated. |
| Clear Foci around pole cell nuclei. |
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| 15 ms |
| 51 ms |
| 25 ms |
| 15 ms No zygotic foci, perhaps due to masking by signal from cytoplasmic RNA. |
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| Good probe. |
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| 84 ms I think it is all zygotic, nothing sub-cellular (?). I wish we had 40x images. |
| 84 ms |
| 84 ms |
| 84 ms Re-annotated by Ronit- 2014. |
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| -performed via HCR-FISH using 3 probe sets targeting common exons |
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| -U2 us encoded at at least 6 different loci which this probe tries to target -U2: 34ABa 2L CR31850, U2: 34ABb 2L CR31854, U2: 34ABc 2L CR33788, U2: 38ABb 2L CR32878, U2: 38ABa 2L CR32882, and U2: 14B X CR32913 |
| -testing via HCR-FISH using a single probe set common to all listed loci |
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| -also known as scaRNA:MeU5-C46 |
| -tested using HCR-FISH using a 5 probe sets |
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| Re-annotated by Ronit. |
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| Good probe on gel. |
| Very interesting expression |
| I see strong peri-nuclear expression around the yolk nuclei, and 'podia' as well. |
| Very interesting expression! |
| Salivary glands, probably non-specific. |
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| No RNA in pole cells. |
| No probe information on the gel. |
| Partly nascent transcription, partly sub-nuclear localization. |
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| Good probe on gel. Nice insitu. |
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| Very interesting pattern. |
| Re-annotated |
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| Images by Eric's team. |
| -missing coverage of late stages and is a good candidate for retesting (2025) |
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| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |