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Number of genes | 42 | |
Export |
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insitu done in 2006 by Eric's group. |
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Re-annotated 2014. |
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Re-annotated |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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Partly nascent, partly sub-nuclear. |
Lowish probe on gel. |
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Good probe on gel. |
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Good probe on gel. |
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Re-annotated. |
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Good probe on gel. |
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Low probe on gel. |
Nice patterns! |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-interesting 7-stripe pattern observed in the blastoderm/epidermis |
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Stripes. |
Good probe on gel.________ Important note: these images are from probe RH01620 from plate AM25 (RA.324), position A8 (8) Same CG number (CG8050) |
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The wingless probe is on the next row of the same plate... Potential cross-contamination. |
Good probe on gel. |
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Not all Nascent... Some nuclei have clearly one big dot (not two), sometimes right at the edge of the DAPI staining. Also, some cytoplasmic foci. |
Good probe on gel. |
Not all Nascent... Some nuclei have clearly one big dot (not two), sometimes right at the edge of the DAPI staining. Also, some cytoplasmic foci. Salivary glands, probably non-specific. |
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Re-annotated |
Neuroblasts |
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Good probe on gel. |
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Good probe on gel. |
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Clear patches of cells close to the invaginating tracheal placodes. |
Good probe on gel. RNA is allover, with some small but clear foci. |
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Re-annotated |
Need more photos for a better description. |
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-missing late stage expression patterns, worth retesting to get full picture (2024) |
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-probes were designed against eve-RA isoform transcript |
-tested using HCR-FISH probes (3 probe sets) |
-very neat stripe patterns |
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Re-annotated. |
Need more images for a more complete description. |
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re-annotated |
Amnioserosa cells. |
Need more photos for a better description. in situ done in 2006. |
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Low probe on gel. |
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It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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Good probe on gel, a bit low. |
Apical expression stops at the posterior -interesting-. |
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Zygotic RNA exhibits diffuse apical localization. During nuclear divisions, the RNA localizes in cytoplasmic foci. |
-appears to be missing some late stage coverage, candidate for retesting (2025) |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-retested recently for late stage coverage (2025); appears to be expressed in a different program in the gnathal elements and sensory regions in late stages |
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Male reproductive tissues |
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It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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Small, clear foci. |
Good probe on gel. Some photos from a different day. |
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Good probe on gel. |
Nice stripes. |
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It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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Neuroblast, interesting. |
Nice stripes! |
Lowish, good probe. Very nice insitu! |
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Expressed in Tracheal pits. |
Expressed in Tracheal placodes. |
The probe is low in our gel, compared to other probes. The in situ worked well. |
3d old tissues---Zhantao |