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| Number of genes | 45 | |
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| Very low probe on gel. |
| Very interesting patterns. |
| Salivary glands, probably non-specific. |
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| Expression is maintained in germ cells following gastrulation. |
| RNA localization is very dynamic, ranging from perinuclear, to apical, and finally pole cell enrichment. |
| RNA exhibits perinuclear localization syggesting association with microtubules. |
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| Salivary glands, probably non-specific. |
| The probe looks good on gel The photos are from a combination of 2 experiments. One had a brighter probe than the other, which explains why some photos are brighter than others. |
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| asymmetric distribution in neuroblasts |
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| Good strong probe. |
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| Zygotic RNA diffusely localized, no obvious sites of zygotic expression past stage 5. |
| Zygotic expression occurs early in stage 4. Zygotic RNA accumulates around nuclei and chromosomes at metaphase. |
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| -probes were designed against RA isoform |
| -tested using HCR-FISH probes (3 probe sets) |
| -expression pattern aligns well with modENCODE RNA-seq data -observed possible early lateral or dorsal-ventral localization of early ectoderm |
| -bright foci is mostly background |
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| Good, strong probe on gel. |
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| Re-annotated |
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| -performed via HCR-FISH using 2 probe sets targeting the RA isoform transcript |
| -this non-coding gene is an extremely highly expressed lncRNA in male testes according to scRNA-seq data, and exhibits a strong nuclear pattern in in embryo yolk, pole cell, and random blastoderm cells |
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| -performed via HCR-FISH using 3 probe sets targeting the RC isoform transcript |
| -the gene is a lncRNA with quite interesting patterns in the CNS (brain and VNC) |
| -seems to be expressed in the anterior transverse furrow, shown in stage 6-7 images |
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| Good probe on gel. |
| Maybe membranes... need better data. |
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| Good probe on gel. |
| Sub and peri nuclear with apical enrichment. Anterior cells only. Very specific and interesting. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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| Borderline between 'not expressed' and 'low expression' |
| Re-annotated by Ronit |
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| Good probe on gel Pole plasm enriched, very strong expression (3ms photos). Mild gradient Posterior to Anterior. |
| Posterior to anterior gradient. |
| Very specific and dynamic expression. Perinuclear. Salivary glands, probably non-specific. |
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| Re-annotated 2014 |
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| Male reproductive tissues |
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| Good probe. Very nice insitu patterns! |
| Data from RE10437 probe. |
| Intra-nuclear in balstoderm and yolk nuclei. |
| Intranuclear localization. Amnioserosa specific pattern. etc. Salivary glands and tracheal lumen, probably non-specific. |
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| Re-annotated RW |
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| Nice patterns. |
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| -detected using HCR-FISH protocol (3 probe sets) |
| -probes were designed against dap-RA isoform transcript |
| -really neat expression patterns |
| -images are from two separate experiments under identical conditions for better coverage |
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| Central yolk nuclei. |
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| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -neat and diverse expression patterns throughout development |
| -images are from two separate experiments under identical conditions for better coverage |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -expression seen in the stomatogastric sensory primordium, and the anterior and posterior midgut primordium; forms a complex along with ac (CG3796) so should colocalize in neuronal regions |
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| Apical excluded in some embryos. |
| re-annotated |
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| -tested via HCR-FISH using 3 probe sets |
| -targeted the transcript isoform sqh-RC |
| -retested to provide images of ubiquity; also expressed in salivary glands in late embryos but that may be non-specific probe binding |
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| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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| Male reproductive tissues |
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| Need more images to get the full story. In situ done before we started screening late stage embryos. |
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| -retested using HCR-FISH 2025 for full coverage of late stage expression patterns |
| -performed using 3 probe pairs (HCR-FISH) against the RB isoform |
| -really interesting patterns! |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -neat expression patterns in early embryos and early germ band elongation stages; expression is no longer detectable after stage 9-10 (fits modENCODE RNA-seq data well) |