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| Number of genes | 15 | |
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| I wish I had images from stage 10 and older :( |
| Re-annotated |
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| The RNA is apically enriched and seems to be both diffusely localized and also forms dicrete cytoplasmic foci. |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
| -images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
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| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -very diverse expression patterns in the late embryos; possibly expressed in the germ cell |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -no longer detected after stages 6-7, probably expressed weakly and ubiquitously at all later stages |
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| Note: this in situ was done twice with the same result The next gene on the plate has a similar expression pattern |
| Nice anterior to posterior gradient. Apical expression. Cytoplasmic foci also in a gradient anterior to posterior. Very interesting. |
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| Central nervous system |
| Anterior gradient. |
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| -performed via HCR-FISH using 3 probe sets targeting the RB isoform transcript |
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| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -neat and diverse expression patterns throughout development |
| -images are from two separate experiments under identical conditions for better coverage |
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| -retested using HCR-FISH 2025 for full coverage of late stage expression patterns |
| -performed using 3 probe pairs (HCR-FISH) against the RB isoform |
| -really interesting patterns! |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -neat expression patterns in early embryos and early germ band elongation stages; expression is no longer detectable after stage 9-10 (fits modENCODE RNA-seq data well) |