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Result Summary
| Number of genes | 9 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Basal localization Basal enrichment Apical localization Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen CNS Ventral nerve cord Perinuclear VNC Brain Bolwig's organ Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Random cortical nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Embryonic localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Yolk cortex exclusion |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Yolk cortex exclusion Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Yolk cortex exclusion Apical exclusion Basal localization Basal enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Sensory system Salivary glands Salivary glands lumen Bolwig's organ Blastoderm nuclei Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| -candidate for retesting for more accurate results (2025) |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
Stage 4-5 Expressed Degraded Degraded partially stage 5 Degraded partially stage 4 Degradation maternal transcripts |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Bolwig's organ |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in gastric caeca Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Not expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Expression level-TE Expressed Low - TE Testis Cell type and stage - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Cytoplasmic foci in spermatogonium - TE Spermatocytes -TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Individualizing Spermatids - TE Cytoplasmic in individualizing spermatids - TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Early elongating spermatids-TE Cytoplasmic in early elongating spermatids Cyst cells -TE Tail cyst cell - TE Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Distal tip region-TE Expressed in distal tip region - TE Seminal Vesicle Subcellularly localized - SV Lumenal-SV Non-particulate - SV Ejaculatory Duct Subcellularly localized - ED Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Low - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC Main cells -AG Basal Enrichment - MC |
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Yolk plasm exclusion |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Fat body Bolwig's organ Bolwig's organ axon Localized Subcellular localization patterns Cell junction associated Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic CNS Ventral nerve cord Axons Bolwig's organ |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript-RA |
| -late stages show higher background than ideal but we are confident in our annotations being real; not sure if maternal signal observed in previous experiments is real |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Bolwig's organ Bolwig's organ axon Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Foci Small Foci |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Particular proventriculus area Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - Perinuclear Malpighian tubules - Half moon perinuclear Malpighian tubules - foci Malpighian tubules allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs few cells - dots Leg discs allover Expressed in eye disc Eye disc allover Expressed in antennal disc Antennal disc few cells - dots Antennal disc allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe, a bit low. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS CNS Brain Bolwig's organ |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -BDGP reported ring gland expression, not observed here |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Maternal Localized Subcellular localization patterns Exclusionary patterns Cytoplasmic foci Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Degraded Degraded completely stage 8 Degradation maternal transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Ring gland Optic lobes primordium Head CNS Brain Bolwig's organ Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Need more images. |
| Small foci. |
| Allover with small foci. Re-annotated. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Bolwig's organ Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| No probe. |
| Very nice insitu!!! |
| No probe |