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Number of genes | 15 | |
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The transcript is enriched at the basal part of the nuclei (dorsal developing epithelial cells... pre-amnioserosa). It is also enriched at the yolk cortex. Very interesting. |
Very nice patterns. |
Very nice patterns. |
Amnioserosa cells. It looks like a membrane. Also the anterior and posterior midgut cells. |
Good probe on gel. |
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Good probe. |
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Nice amnioserosa pattern. |
Good probe on gel. |
Two images show the same embryo with different exposure. |
Nice amnioserosa patterns. |
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Good probe on gel. |
Interesting amnioserosa pattern. |
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Good probe on gel |
Interesting RNA localization patterns |
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Salivary glands, probably non-specific. |
Good probe on gel. Great RNA localization patterns. |
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Amnioserosa specific in embryo |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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Good probe on gel. |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-diverse ectoderm derived structures such as the salivary gland (and the salivary primordium), amnioserosa, proventriculus, tracheal pits, and epidermis |
-prior reported expression of hemocytes were not visible in this experiment |
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The RNA also seems to accumulate apically in neuroblasts, with potential transition to basal. |
Zygotic expression within cells that form furrows. Interesting localization of RNA in amnioserosa. |
Zygotically expressed RNA forms clusters in apical cytoplasm. |
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Good probe on gel. Data from RE17418 probe |
Salivary glands, probably non-specific. |
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Anterior and posterior midgut. |
Good probe on gel. All over low, with some small foci... low but clear. |
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Good probe on gel. |
Salivary glands, probably non-specific. |
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Male reproductive tissues |
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Good probe. Very nice insitu patterns! |
Data from RE10437 probe. |
Intra-nuclear in balstoderm and yolk nuclei. |
Intranuclear localization. Amnioserosa specific pattern. etc. Salivary glands and tracheal lumen, probably non-specific. |
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Salivary glands, probably non-specific. |
Good probe on gel (strong). |