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Result Summary
| Number of genes | 13 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Apical localization Apical enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Strong expression (1-20ms exp) |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Proventriculus Pharynx Muscles-embryo Visceral mesoderm Midgut Hindgut Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around midgut nuclei Perinuclear around hindgut nuclei Cell junction associated |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| semi-good probe on gel. |
| Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
|
| stage 6-7 |
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
|
| stage 8-9 |
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Posterior spiracles Hindgut Localized Subcellular localization patterns Perinuclear localization Perinuclear around hindgut nuclei |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Nascent transcript in malpighian tubules Malpighian tubules allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Expressed in leg discs Leg discs particular pattern |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes allover Brain lobes - Significant area |
| ovary and testis |
|
3rd Instar Larval Tissues Larval Ovary and Testis-Larva Not expressed in larval testis |
Adult
| Area | Images | Terms |
|---|
Comments
| Note: some images from RE25729 probe. |
| Good probe on gel. |
| Hindgut |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Posterior spiracles Hindgut Blastoderm nuclei Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear around hindgut nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Salivary glands, probably non-specific. |
| Borderline between 'not expressed' and 'low expression'. |
| Good probe on gel Borderline between 'not expressed' and 'low expression'. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Posterior localization Exclusionary patterns Pole cell exclusion Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Basal localization Basal enrichment Apical localization Apical enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in API Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Nuclei-associated localization Germ cell localization Germ cell enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Midgut Midgut subset Hindgut Specific hindgut area or cells Head Fat body Dorsal vessel Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear around midgut nuclei Perinuclear around hindgut nuclei Perinuclear around fat body nuclei Nuclei-associated localization Accumulation nuclear subregion Cell junction associated |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Anterior and posterior midgut |
| Good probe, nice in situ. |
| Anterior and posterior midgut |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Membrane associated Cell junction associated Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Localized Subcellular localization patterns Membrane associated |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Posterior spiracles Specific posterior spiracle pattern Pharynx Midgut Midgut ICPs Malpighian tubules Hindgut CNS Ventral nerve cord Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear around midgut nuclei Perinuclear around hindgut nuclei Membrane associated Apical enrichment Apical in pharynx |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| I see cell membrane dots. I can even see double membrane in some cases (not certain until a membrane marker is used...). Interesting localization. |
| Salivary glands and tracheal lumen, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Proventriculus Posterior spiracles Pharynx Hindgut Specific hindgut area or cells Foregut Ectoderm Polarized Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around hindgut nuclei Perinuclear around ectoderm nuclei Foci Small Foci Small Foci in hindgut Cell junction associated Apical enrichment Apical enriched hindgut Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe, good insitu. Interesting late expression/ locaization. |
| Nice late expression/ localization |
| Small foci, low expression. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Non-Expressed Degraded Degraded completely stage 7 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Segmented pattern Salivary glands Proventriculus Pharynx Midgut Malpighian tubules Hindgut Specific hindgut area or cells Foregut Esophagus Ectoderm Dorsal vessel CNS Ventral nerve cord Foci - VNC Blastoderm nuclei Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around tracheal nuclei Perinuclear around midgut nuclei Perinuclear around malpighian tubules Perinuclear around hindgut nuclei Perinuclear around ectoderm nuclei Foci Small Foci Small foci in Proventriculus Small Foci in CNS Cell junction associated Apical enrichment Apical enriched midgut Apical enriched hindgut Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe. Nice pattern. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Non-Expressed Degraded Degraded completely stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Proventriculus Posterior spiracles Specific posterior spiracle pattern Midgut Midgut subset Hindgut Blastoderm nuclei Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around hindgut nuclei Foci Small Foci Small Foci in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Data from RE21493 probe!!! Good probe on gel. Blasted RE21493 sequence on FlyBase - 100% correspondence with the Cpr transcript. Please confirm. |
| Very low expression. |
| Salivary glands and tracheal lumen, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Yolk nuclei Subset yolk nuclei Anterior yolk nuclei Weak expression (>41ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Anterior yolk nuclei Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion Basal localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around pole cell nuclei Membrane associated Embryonic localization patterns Ventral localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Ventral yolk nuclei Anterior yolk nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Strong expression (1-20ms exp) Localized Yolk plasm localization Yolk plasm enrichment Germ cell localization Germ cell enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Foregut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around midgut nuclei Perinuclear around hindgut nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around all nuclei Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Pole cell localization Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Apical exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei All pole cell nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around all nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Foregut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around midgut nuclei Perinuclear around hindgut nuclei Foci Small Foci Small Foci in midgut Small Foci in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 7 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 8 Degradation maternal transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Proventriculus Posterior spiracles Pharynx Hindgut Specific hindgut area or cells Foregut Esophagus Ectoderm Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear around hindgut nuclei Perinuclear around esophagus nuclei Cell junction associated |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body Borderline between 'not expressed' and 'low expression' |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe. |
| Need better resolution images to determine exact sub-cellular localization. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in ectoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Optic lobes primordium Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Maxillary complex Hindgut Specific hindgut area or cells Esophagus CNS Ventral nerve cord Perinuclear VNC Foci - VNC Clypeo-labral bud Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around hindgut nuclei Perinuclear around esophagus nuclei Foci Small Foci Small Foci in hindgut Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed using HCR-FISH (3 probe sets) |
| -probes targeted dac-RB isoform of transcript |
| -very neat patterning in later stages; possibly visceral muscles of the hindgut |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands - specific region Proventriculus Hindgut Foregut Esophagus Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around salivary gland nuclei Perinuclear around hindgut nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe... Nice in situ. |
| Nice and clear. |
| Borderline between 'not expressed' and 'low expression'. |