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Result Summary
| Number of genes | 29 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei All pole cell nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Strong expression (1-20ms exp) Medium expression (21-40ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Ubiquitous Strong expression (1-20ms exp) Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei Foci Small Foci Small Foci in midgut Small Foci in foregut Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -sample background seems to high and does not match current data - candidate for retest (2025) |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Yolk cortex exclusion Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Maternal |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Pharynx Muscles-embryo Midgut Midgut subset Ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around midgut nuclei Perinuclear 'rings' Other subcellular pattern Nuclei-associated localization Nuclear in midgut nuclei Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area Ventral nerve cord - foci or 'dots' |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Expressed in lymph glands Lymph glands all over |
Adult
| Area | Images | Terms |
|---|
Comments
| Photos by someone else... |
Probe SD10530 Links: Permalink
Genes: BcDNA:SD10530
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Cell division apparatus Other cell division pattern Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation entire nucleus Germ cell localization Germ cell enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Posterior spiracles Pharynx Midgut Hemocytes Head Ectoderm Brain Localized Subcellular localization patterns Nuclei-associated localization Nuclear in pharynx Nuclear in other cells Nuclear in midgut nuclei Nuclear in hemocytes Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Nuclear and sub-nuclear localization. |
| Nuclear. |
| Nuclear and sub-nuclear localization. |
| Very nice pattern!! |
| Very low probe on gel. Great in situ... No transcript associated with this probe, it may be a copia transposon element. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Dorsal blastoderm nuclei Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in API Localized Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Basal localization Basal enrichment Posterior localization Pole cell localization Local terms for zygotic transcripts Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in API Anterior blastoderm nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Midgut Head Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in tracheal nuceli Nuclear in midgut nuclei Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Re-annotated. insitu done in 2007 by Eric's group |
| Anterior and posterior midgut cells. |
| Need more images to get the complete story. insitu done in 2007 (7 years ago), when the screen was focusing on stages 1 to 9 only. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Salivary glands Salivary glands lumen Posterior spiracles Pharynx Midgut Midgut subset Hindgut Midgut hindgut border Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in other cells Nuclear in midgut nuclei Accumulation nuclear subregion Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Maybe a few small foci. |
| Subnuclear localization. 2R - two dots, not one. |
| Good probe on gel. |
| Maybe a few foci. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Proventriculus Midgut Midgut subset Malpighian tubules Hindgut Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around midgut nuclei Perinuclear around malpighian tubules Nuclei-associated localization Nuclear in midgut nuclei Accumulation nuclear subregion Foci Small Foci Small Foci in midgut Small Foci in amnioserosa Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| One dot per nuclei - subnuclear localization. |
| Very clear example. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns |
| stage 6-7 |
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
|
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Posterior spiracles Pharynx Muscles-embryo Visceral mesoderm Muscles - subregion Midgut Midgut subset Midgut ICPs Hindgut Specific hindgut area or cells Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around muscle nuclei Perinuclear around midgut nuclei Nuclei-associated localization Nuclear in pharynx Nuclear in other cells Nuclear in midgut nuclei Nuclear in hindgut nuclei Nuclear in ectoderm Accumulation nuclear subregion Foci Small Foci Small Foci in muscle cells Small Foci in midgut Basal enrichment Basal in hindgut Apical enrichment Apical enriched hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Dorsal blastoderm nuclei Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Salivary glands Salivary glands lumen Proventriculus Midgut Midgut subset Midgut gradient Hindgut Specific hindgut area or cells Fat body Fat body- specific region Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around midgut nuclei Nuclei-associated localization Nuclear in midgut nuclei Accumulation nuclear subregion Foci Small Foci Small Foci in midgut Apical enrichment Apical enriched midgut Apical enriched hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk plasm localization Posterior localization Pole cell localization Pole cell enrichment Exclusionary patterns Apical exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Maternal Localized Subcellular localization patterns Other subcellular pattern Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns |
| stage 8-9 |
|
Stage 8-9 Expressed Maternal Localized Germ cell localization Germ cell enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Proventriculus Posterior spiracles Specific posterior spiracle pattern PNS Muscles-embryo Muscles - subregion Midgut Hindgut Foregut Ectoderm Blastoderm nuclei Atrium Localized Subcellular localization patterns Nuclei-associated localization Nuclear in muscle cells nuclei Nuclear in midgut nuclei Nuclear in hindgut nuclei Nuclear in ectoderm Accumulation nuclear subregion Foci Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Partly nascent, partly nuclear subregion. |
| done twice. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Midgut subset Midgut ICPs Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around midgut nuclei Nuclei-associated localization Nuclear in midgut nuclei Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| It is all over, with some cytoplasmic 'foci'. |
| Interesting and specific expression in part of the embryonic midgut. At stage 14, I also see the instertitial cell progenitors (ICPs), and other cells (including the cuprophilic cells). |
| Small foci.... real. |
| Good probe on gel (a bit low). |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Midgut Midgut subset Midgut ICPs Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Salivary glands, probably non-specific. Nuclear in ICPs |
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Exclusionary patterns Apical exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei All pole cell nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Accumulation in all nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Foregut Ubiquitous Strong expression (1-20ms exp) Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Foci Small Foci Small Foci in midgut Small Foci in foregut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in all nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in all nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Ubiquitous Strong expression (1-20ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Foregut Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei All pole cell nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Ubiquitous Strong expression (1-20ms exp) Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei Foci Small Foci Small Foci in midgut Small Foci in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Probe CR43314-RA Links: Permalink
Genes: CR43314
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Central band of nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Pole cell exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Anterior blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Expression in API Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Posterior spiracles Specific posterior spiracle pattern PNS Midgut Midgut subset Head Head epidermis Clypeo-labral bud Anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in midgut nuclei Nuclear in ectoderm Foci Small Foci Small Foci in PNS Small Foci in ectoderm Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
| -images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Ventral yolk nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Ventral yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in amnioserosa Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary gland nuclei Midgut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in salivary glands Nuclear in midgut nuclei Foci Small Foci Small Foci in salivary glands Small Foci in other tissues |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Ventral yolk nuclei Germ cell nuclei All pole cell nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Ubiquitous Strong expression (1-20ms exp) Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Foci Small Foci Small Foci in midgut Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Germ cell nuclei All pole cell nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Foregut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei Foci Small Foci Small Foci in midgut Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Foci Small Foci Small Foci in midgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Ubiquitous Strong expression (1-20ms exp) Medium expression (21-40ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Foregut Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Foci Small Foci Small Foci in midgut Small Foci in foregut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Germ cell nuclei All pole cell nuclei Ubiquitous Medium expression (21-40ms exp) |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Ubiquitous Strong expression (1-20ms exp) Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei Foci Small Foci Small Foci in midgut Small Foci in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
|
Stage 6-7 Non-Expressed |
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Midgut subset Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Foci Small Foci Small Foci in midgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -BDGP observed maternal signal, however this was not observed in our experiment |
| -Possible dorsal vessel or visceral muscle signal at late stage 12 |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Posterior spiracles Pharynx Muscles-embryo Visceral mesoderm Muscles - subregion Midgut Hindgut Germ cell nuclei Gonads Esophagus Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in muscle cells nuclei Nuclear in midgut nuclei Nuclear in hindgut nuclei Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Partly nascent, partly sub-nuclear. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei Foci Small Foci Small Foci in midgut Small Foci in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in amnioserosa All blastoderm nuclei Persistent widespread Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Inside Yolk nuclei Midgut Hindgut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Dorsal yolk nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Foregut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei Foci Small Foci Small Foci in hindgut Small Foci in foregut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around yolk nuclei |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei All pole cell nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Foci Small Foci Small Foci in midgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei All yolk nuclei Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Exclusionary patterns Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Foregut Localized Subcellular localization patterns Nuclei-associated localization Nuclear in midgut nuclei Nuclear in hindgut nuclei Foci Small Foci Small Foci in midgut Small Foci in hindgut Small Foci in foregut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|