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Result Summary
| Number of genes | 27 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Sensory system Salivary glands Posterior spiracles Specific posterior spiracle pattern PNS Hindgut Specific hindgut area or cells Midgut hindgut border Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in other cells Accumulation entire nucleus Foci Small Foci Small Foci in tracheal cells |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in other cells Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Borderline between 'not expressed' and 'low expression' |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed |
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern Proventriculus Posterior spiracles PNS Head Brain Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Borderline between 'not expressed' and 'low expression'. |
| Sub-nuclear. |
| Good probe. Borderline between 'not expressed' and 'low expression'. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Head Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Nascent transcript in salivary gland Expressed in salivary glands Salivary glands allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe on gel. Good in situ reaction and data Small foci. |
| Clear, small foci' Nascent in head - particular cells. |
| Clear, small foci or puncta. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
Stage 4-5 Non-Expressed Degraded Degraded completely stage 5 Degradation maternal transcripts |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus Foci Small Foci Small Foci in CNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS CNS Ventral nerve cord Foci - VNC Brain Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in CNS Foci Small Foci Small Foci in PNS Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -expression appears quite ubiquitous, but closer examination reveals enrichment in CNS and head sensory (maybe antennal-maxillary complex?) and trunk PNS |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Dorsal yolk nuclei Germ cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Germ cell localization Germ cell enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Salivary glands PNS Pharynx Hemocytes Germ cell nuclei Gonads Ectoderm CNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Perinuclear around hemocytes Nuclei-associated localization Nuclear in PNS Nuclear in hemocytes Nuclear in ectoderm Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| The probe looks good on gel. |
| Dynamic expression. Salivary glands, probably non-specific |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen PNS Midgut Head Fat body Ectoderm Blastoderm nuclei Atrium Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in ectoderm Accumulation nuclear subregion Foci Small Foci Small Foci in ectoderm Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Salivary glands, probably non-specific Sub-nuclear localization. |
| Maybe lower at the pole cells and lower apical... |
| Low probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Sensory system Salivary glands Posterior spiracles PNS Pharynx Hindgut Dorsal vessel Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around pharynx nuclei Nuclei-associated localization Nuclear in PNS Accumulation entire nucleus Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Nuclear Sensory system. Salivary glands, probably non-specific. |
| Small foci. |
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands Posterior spiracles Specific posterior spiracle pattern PNS Head Head epidermis Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Not expressed in larval muscles - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Not expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe on gel. |
| Interesting anterior late embryo expression and sensory neurones. |
Probe CR43314-RA Links: Permalink
Genes: CR43314
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Central band of nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Pole cell exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Anterior blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Expression in API Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Posterior spiracles Specific posterior spiracle pattern PNS Midgut Midgut subset Head Head epidermis Clypeo-labral bud Anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in midgut nuclei Nuclear in ectoderm Foci Small Foci Small Foci in PNS Small Foci in ectoderm Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
| -images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 7 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Sensory system Salivary glands Salivary glands lumen PNS CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in other cells Nuclear in CNS Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Expressed in malpighian tubules Malpighian tubules allover Expressed in gastric caeca Subcellular localization in gastric caeca Few gastric caeca foci Gastric caeca allover Borderline between 'not expressed' and 'low expression' |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Brain lobes allover Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Not expressed in ring gland Lymph Glands Not expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Individualizing Spermatids - TE Waste Bag - TE Cyst cells -TE Tail cyst cell - TE Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Distal tip region-TE Expressed in distal tip region - TE Lower expression in tip region Cytoplasmic Seminal Vesicle Expression level-SV Not Expressed - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Expression level-ED Expressed Medium - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Apical Enrichment-ED Accessory Gland Subcellularly localized - AG Lumenal - AG Diffuse foci-AG Expression level-AG Expressed Low - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Main cells -AG Cytoplasmic-MC Basal Enrichment - MC |
Comments
| Salivary glands and tracheal lumen, probably non-specific. |
| Good probe on gel. This insitu was done twice... similar reslutls obtained. |
| Important note: The probe LP11739 overlaps with the 5' UTR of the 2mit gene (same location). |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 9 Degradation maternal transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern PNS CNS Brain Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Accumulation nuclear subregion Foci Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Foci. |
| Partly nascent, partly sub-nuclear. |
| Very low probe on gel Some small foci. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Random cortical nuclei Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic PNS Brain Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Lateral cord neuron. Very specific set of cells. |
| Good probe on gel Zygotic stage 3. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Local terms for zygotic transcripts Embryonic localization patterns Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Local terms for zygotic transcripts Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Localized Subcellular localization patterns Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Perinuclear around blastoderm nuclei Nuclei-associated localization Local terms for zygotic transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Sensory system PNS Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Accumulation nuclear subregion Accumulation entire nucleus |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Dorsal blastoderm nuclei Weak expression (>41ms exp) |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Dorsal blastoderm nuclei Weak expression (>41ms exp) Localized Embryonic localization patterns Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Embryonic localization patterns Dorsal localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Posterior spiracles Specific posterior spiracle pattern PNS Oenocytes Midgut Hindgut Specific hindgut area or cells Head CNS Ventral nerve cord Brain Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in other cells Nuclear in ectoderm Nuclear in CNS Nuclear in amnioserosa Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Posterior blastoderm nuclei Central band of nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in ectoderm Expression in amnioserosa Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei PNS Hemocytes Head Ectoderm Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Foci - VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in other cells Nuclear in CNS Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel No gene associated. |
| Partly nascent, partly nuclear subregion |
| Partly nascent, partly nuclear subregion |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Terminal-gene pattern Gap-gene pattern Localized Subcellular localization patterns Ventral localization Posterior localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Membrane associated Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Exclusionary patterns Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Other subcellular pattern Nuclei-associated localization Intranuclear accumulation |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Ring gland Proventriculus PNS Neuroblasts Polarized Neuroblasts Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Muscles - subregion Midgut Midgut subset Midgut ICPs Hindgut Head Fat body Ectoderm Polarized Ectoderm CNS Ventral nerve cord Nuclear - VNC Axons Brain Blastoderm nuclei Atrium Anal pad Localized Subcellular localization patterns Perinuclear localization Nuclei-associated localization Nuclear in PNS Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus Cell extensions or 'podia' Apical enrichment Apical enriched midgut Apical enriched hindgut |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel Maybe some small foci. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Posterior spiracles PNS Pharynx Muscles-embryo Muscles - subregion Fat body CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Foci Small Foci Small Foci in Pharynx Small Foci in muscle cells Small Foci in CNS Small Foci in Brain |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Sensory system Salivary glands Salivary glands lumen PNS Head Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Nuclei-associated localization Nuclear in PNS Accumulation nuclear subregion Accumulation entire nucleus Foci Small Foci Small Foci in other tissues Small Foci in amnioserosa |
Larva
| Area | Images | Terms |
|---|---|---|
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc particular pattern Eye disc - center round cells |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area Brain lobes - Foci or dots |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Single stripe Central band of nuclei Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Basal localization Basal enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Anterior blastoderm nuclei Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic PNS Germ cell nuclei Ectoderm CNS Ventral nerve cord Polarized VNC Perinuclear VNC Nuclear - VNC Axons Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in CNS Accumulation nuclear subregion Accumulation entire nucleus Foci Bigger Foci Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
| Salivary glands, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Single stripe Expression in ectoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Degraded Degraded partially stage 7 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Single stripe Expression in ectoderm Anterior blastoderm nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands lumen PNS Head Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Axons Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in CNS Foci Small Foci Small Foci in PNS Small Foci in head Small Foci in CNS Small Foci in Brain Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Not every embryo has a pole cell excluded pattern. |
| Head, specific. |
| Good probe on gel. Small, localized foci. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Basal localization Basal enrichment Pole cell localization Pole cell enrichment Embryonic localization patterns Degraded Degraded partially stage 7 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Germ cell localization Germ cell enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern Salivary glands Salivary glands lumen PNS Oenocytes Muscles-embryo Muscles - subregion Midgut Hindgut Specific hindgut area or cells Head Germ cell nuclei Gonads Fat body Ectoderm Clypeo-labral bud Blastoderm nuclei Anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Accumulation nuclear subregion Accumulation entire nucleus Foci Small Foci Small Foci in midgut Small Foci in fat body Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in hindgut Subcellular localization in hindgut Hindgut foci Few hindgut foci Hindgut basal enrichment Hindgut allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes allover Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Expressed in salivary glands Salivary glands allover |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Salivary glands, probably non-specific. |
| Good probe on gel. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
Stage 1-3 Non-Expressed |
|
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system PNS Ectoderm Anal pad Ring around anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in ectoderm Foci Small Foci Small Foci in PNS Small Foci in ectoderm Bigger Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RS isoform transcript (this particular gene has 60 recorded isoforms on FlyBase - further optimization of transcript probe targeting and possibly more probe sets should be considered if a stronger signal is desired) |
| -weak but highly dynamic PNS expression patterns, possibly can be better captured by increasing the probe sets |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Random cortical nuclei Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 4-5 |
|
Stage 4-5 Expressed Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system CNS Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in CNS Foci Small Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Non-Expressed |
| stage 4-5 |
|
Stage 4-5 Non-Expressed |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Salivary glands PNS CNS Ventral nerve cord Foci - VNC Axons Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in CNS Accumulation nuclear subregion Foci Small Foci Small Foci in CNS Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Salivary glands and tracheal lumen, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic PNS Head Ectoderm CNS Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Nuclei-associated localization Nuclear in PNS Nuclear in other cells Nuclear in ectoderm Nuclear in CNS Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Allover with small foci. |
| Sub-nuclear. |
| Good probe on gel. Allover with small foci. |