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Result Summary

Number of genes	43
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Probe RE12073 Links: Permalink

Genes: CG46280

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Ubiquitous Maternal
stage 6-7		Stage 6-7 Expressed Ubiquitous Maternal
stage 8-9		Stage 8-9 Expressed Ubiquitous Maternal
stage 10-17		Stage 10-17 Expressed Zygotic Salivary glands Muscles-embryo Hindgut Midgut hindgut border Head Fat body Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in muscle cells Small Foci in head Small Foci in fat body Small Foci in ectoderm

Larva

Area	Images	Terms
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - Basal arc Malpighian tubules allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc few cells - dots Eye disc basal
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Nuclear localization Lymph Glands - Subnuclear localization Lymph Glands - Cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Distal tip region-TE Not Expressed in distal tip region -TE Seminal Vesicle Expression level-SV Not detected - SV Ejaculatory Duct Subcellularly localized - ED Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Basal Enrichment- ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Low - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC Basal Enrichment - MC

Comments

Good probe on gel.

Salivary glands, probably non-specific.

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions'

Interesting adult testis localization pattern.

Probe SD03066 Links: Permalink

Genes: CG13868

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Ubiquitous Maternal
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Head Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in head Small Foci in amnioserosa

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Tracheal placodes.

Re-annotated.

Probe RE09732 Links: Permalink

Genes: CG14670

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Head Ectoderm CNS Ventral nerve cord Foci - VNC Brain Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in head Small Foci in ectoderm Small Foci in CNS Small Foci in Brain Small Foci in amnioserosa

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus - Few foci Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Ventral nerve cord - allover
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Spermatocytes -TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Distal tip region-TE Expressed in distal tip region - TE Seminal Vesicle Subcellularly localized - SV Cell type - SV Basal Enrichment-SV Ejaculatory Duct Subcellularly localized - ED Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Clusters-AG Expression level-AG Expressed Low - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Main cells -AG Cytoplasmic-MC Cytoplasmic Foci - AG

Comments

Good probe on gel.

Small foci

Salivary glands, probably non-specific.

Very mild enrichment in secondary cells - Adult Accessory Glands

Probe LD24702 Links: Permalink

Genes: CG17286

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Cytoplasmic foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Ubiquitous
stage 8-9		Stage 8-9 Expressed Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Salivary glands Posterior spiracles Specific posterior spiracle pattern Germ cell nuclei Gonads CNS Ventral nerve cord Foci - VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in head Small Foci in CNS Small Foci in Brain

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Nice in situ!

Probe LD47433 Links: Permalink

Genes: CG17724 Kdm4B

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Anterior blastoderm nuclei Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Exclusionary patterns Apical exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in ectoderm Maternal Localized Subcellular localization patterns Exclusionary patterns Apical exclusion Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei
stage 10-17		Stage 10-17 Expressed Zygotic Neuroblasts Head CNS Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Foci Small Foci Small Foci in head Small Foci in CNS

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe LD10058 Links: Permalink

Genes: CG18431

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Non-Expressed
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Blastoderm nuclei Subset blastoderm nuclei Expression in API Anterior blastoderm nuclei
stage 10-17		Stage 10-17 Expressed Zygotic Salivary glands Posterior spiracles Specific posterior spiracle pattern Head Ectoderm Dorsal vessel Dorsal vessel specific pattern Blastoderm nuclei Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in head Small Foci in dorsal vessel

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Re-annotated by Ronit for stage 10-up.

Probe RE06501 Links: Permalink

Genes: CG31100

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Pharynx Midgut Midgut subset Hindgut Head Ectoderm Blastoderm nuclei Anal pad Localized Subcellular localization patterns Foci Small Foci Small Foci in Pharynx Small Foci in hindgut Small Foci in head Small Foci in ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel. Clear, small foci.

One midgut chamber.

Some small foci.

Probe LD12677 Links: Permalink

Genes: CG3136

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization RNA islands Pole plasm Pole buds Nuclei associated localization Perinuclear localization Perinuclear around pole cell nuclei Embryonic localization patterns
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Pole cell localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Exclusionary patterns Apical exclusion Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Pole cell localization Pole cell enrichment
stage 8-9		Stage 8-9 Expressed Zygotic Zygotic starting stage 8 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Germ cell localization Germ cell enrichment
stage 10-17		Stage 10-17 Expressed Zygotic Salivary glands Salivary glands - specific region Pharynx Midgut Head Esophagus Ectoderm Brain Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in salivary glands Small Foci in midgut Small Foci in head Small Foci in foregut Small Foci in ectoderm Small Foci in Brain

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc nuclear localization Eye disc subnuclear localization Eye disc few cells - dots Expressed in antennal disc Antennal disc few cells - dots
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - nuclear dots Nascent transcript in salivary gland Expressed in salivary glands
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva Not expressed in larval ovary
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover

Adult

Area	Images	Terms

Comments

Good probe on gel.

Probe AT21186 Links: Permalink

Genes: CG32081

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Anterior blastoderm nuclei
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Segmented pattern Salivary glands Salivary glands lumen PNS Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Head Ectoderm CNS Ventral nerve cord Brain Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in head Small Foci in ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Salivary glands, probably non-specific.

Lots of specific expression, dynamic and strong.

Good probe in our gel.

Probe RE12054 Links: Permalink

Genes: koi

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Ubiquitous Degraded Degraded partially stage 9
stage 10-17		Stage 10-17 Expressed Zygotic Proventriculus Muscles-embryo Muscles - subregion Midgut Hindgut Head Fat body Esophagus CNS Brain Blastoderm nuclei Anal pad Localized Subcellular localization patterns Nuclei-associated localization Nuclear in muscle cells nuclei Accumulation nuclear subregion Foci Small Foci Small foci in Proventriculus Small Foci in muscle cells Small Foci in head Small Foci in foregut Small Foci in fat body Small Foci in ectoderm Small Foci in CNS Small Foci in Brain

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Fat body - Few and small foci Expressed in fat body Fat body allover - Ubiquitous Borderline between 'not expressed' and 'low expression'
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus - Few foci Proventriculus allover Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules - Basal arc Malpighian tubules allover Expressed in gastric caeca Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva Expressed in heart
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Distal tip region-TE Expressed in distal tip region - TE Lower expression in tip region Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Round Tail Cyst Cell - TE Round Tail Cyst Cell Cytoplasm - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Seminal Vesicle Expression level-SV Not detected - SV Ejaculatory Duct Expression level-ED Expressed Low - ED Subcellularly localized - ED Cell type - ED Cytoplasmic-ED Membrane enriched - ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Low - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC Main cells -AG Cytoplasmic-MC Cytoplasmic Foci - AG

Comments

Good probe on gel.

Probe RE10255 Links: Permalink

Genes: CG33129

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Apical localization Apical enrichment Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed
stage 8-9		Stage 8-9 Expressed Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Polarized tracheal localization Salivary glands Salivary glands lumen Pharynx Hemocytes Hemocytes specific area Head Fat body Fat body- specific region Fat body polarized Ectoderm Polarized Ectoderm Blastoderm nuclei Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around hemocytes Membrane associated Foci Small Foci Small Foci in head Small Foci in ectoderm Apical enrichment Apical trachea Apical ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Proventriculus allover Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules few foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Few hindgut foci Hindgut allover Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Few gastric caeca foci Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc few cells - dots Eye disc allover Expressed in antennal disc Antennal disc few cells - dots Antennal disc allover Borderline between 'not expressed' and 'low expression'
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Cytoplasmic foci Salivary gland - Small foci Expressed in salivary glands Salivary glands allover
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Spermatocyte Tail Cyst Cell - TE Spermatocyte Tail Cyst Cell Cytoplasm - TE Round Tail Cyst Cell - TE Round Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Early cyst cell Cytoplasmic in early cyst cell Cyst stem cell Cytoplasmic in cyst stem cell Seminal Vesicle Subcellularly localized - SV Lumenal-SV Non-particulate - SV Cell type - SV Cytoplasmic-SV Membrane enriched - SV Cytoplasmic Foci - SV Ejaculatory Duct Expression level-ED Expressed Low - ED Subcellularly localized - ED Cell type - ED Cytoplasmic-ED Membrane enriched - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Clusters-AG Expression level-AG Expressed Low - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC Main cells -AG Cytoplasmic-MC

Comments

Good probe on gel.

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions'

Probe RE21703 Links: Permalink

Genes: CG40198

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Anterior blastoderm nuclei
stage 10-17		Stage 10-17 Expressed Zygotic Head Fat body Ectoderm CNS Ventral nerve cord Brain Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in other tissues Small Foci in head Small Foci in fat body Small Foci in ectoderm Small Foci in Brain Small Foci in amnioserosa

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel.

Probe RE27439 Links: Permalink

Genes: CG6634

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Yolk cortex exclusion Pole cell exclusion Apical exclusion Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Pair-rule pattern Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Apical localization Apical enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Posterior spiracles Ectoderm Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Brain Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in other tissues Small Foci in head Small Foci in ectoderm Apical enrichment Apical in other tissues

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Low probe on gel.

Nice patterns!

Probe CG8289-RA Links: Permalink

Genes: CG8289

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Ubiquitous Maternal Degraded Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Expression in ectoderm Expression in API Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Germ cell localization Germ cell enrichment Embryonic localization patterns Germ band localization
stage 10-17		Stage 10-17 Expressed Zygotic Posterior spiracles Muscles-embryo Midgut Hindgut CNS Brain Ubiquitous Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Foci Small Foci Small Foci in other tissues Small Foci in muscle cells Small Foci in midgut Small Foci in hindgut Small Foci in head Small Foci in CNS Small Foci in Brain

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

-probes were designed against RA isoform

-tested using HCR-FISH probes (3 probe sets)

-appears ubiquitous with enrichment in ectoderm, CNS, and posterior spiracles

Probe GM04053 Links: Permalink

Genes: CG8710

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Exclusionary patterns Pole cell exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 9
stage 10-17		Stage 10-17 Expressed Zygotic Proventriculus Pharynx Muscles-embryo Midgut Ectoderm CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Membrane associated Foci Small Foci Small foci in Proventriculus Small Foci in Pharynx Small Foci in midgut Small Foci in head Small Foci in ectoderm Basal enrichment Basal in midgut

Larva

Area	Images	Terms
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in hindgut Subcellular localization in hindgut Hindgut basal enrichment Hindgut allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms

Comments

Good probe on gel.

Probe RE06388 Links: Permalink

Genes: CG32194 CR32194

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Exclusionary patterns Yolk cortex exclusion
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 9 Degradation maternal transcripts
stage 10-17		Stage 10-17 Expressed Zygotic Head Ectoderm CNS Ventral nerve cord Foci - VNC Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in head Small Foci in ectoderm Small Foci in CNS Small Foci in Brain

Larva

Area	Images	Terms
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Distal tip region-TE Not Expressed in distal tip region -TE Testis Cell type and stage - TE Terminal Epithelium - TE Cytoplasmic in terminal epithelium - TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Individualizing Spermatids - TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Sperm Tail Decoration-TCC associated Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Low - TE Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Expression level-SV Expressed Low - SV Cell type - SV Sperm Tail Decor - SV Cytoplasmic-SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Membrane enriched - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Basal Enrichment - MC

Comments

Good probe on gel. Allover with small foci.

Small foci (puncta).

Probe RE53348 Links: Permalink

Genes: CR43470

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Exclusionary patterns Pole plasm excluded
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Dorsal blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Cytoplasmic foci Degraded Degraded partially stage 6 Degradation maternal transcripts
stage 10-17		Stage 10-17 Expressed Zygotic Midgut Head Ectoderm Ubiquitous Localized Subcellular localization patterns Foci Small Foci Small Foci in midgut Small Foci in head Small Foci in fat body Small Foci in ectoderm Small Foci in amnioserosa

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Not expressed in fat body
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Specific testis expression localization Larval testis foci
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Not expressed in lymph glands

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatocytes -TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Individualizing Spermatids - TE Waste Bag - TE Cytoplasmic in individualizing spermatids - TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Lower expression in tip region Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV Expression level-SV Expressed Low - SV Cell type - SV Sperm Tail Decor - SV Cytoplasmic-SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Anterior ejaculatory duct papilla-ED Cytoplasmic in anterior ejaculatory papilla-ED Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Accessory Gland Subcellularly localized - AG Lumenal - AG ECM like pattern-AG Expression level-AG Expressed Low - AG Cytoplasmic Cell type - AG Main cells -AG Cytoplasmic-MC Basal Enrichment - MC

Comments

Good probe on gel.

Nice foci.

Probe LD06565 Links: Permalink

Genes: CR44898 robo2

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Membrane associated Exclusionary patterns Pole cell exclusion Apical exclusion Apical localization Apical enrichment Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Dorsal trunk Dorsal trunk foci Head CNS Ventral nerve cord Foci - VNC Brain Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in head Small Foci in CNS

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Cytoplasmic foci-fat body Expressed in fat body
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Optic lobe Brain lobes - Foci or dots Borderline between 'not expressed' and 'low expression'

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatocytes -TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Individualizing Spermatids - TE Waste Bag - TE Subcellularly localized - TE Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Higher expression in tip region Cytoplasmic Seminal Vesicle Expression level-SV Not Expressed - SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Inner lumen - ED Expression level-ED Expressed Low - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Diffuse localization-ED Basal Enrichment- ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Low - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Cytoplasmic foci-SC Main cells -AG Cytoplasmic-MC Cytoplasmic Foci - AG

Comments

Good probe on gel

Re-annotated

Probe LD19584 Links: Permalink

Genes: Dl

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Posterior blastoderm nuclei Dorsal blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in mesoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in ectoderm
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Dorsal trunk Dorsal trunk foci Segmented pattern Salivary glands Salivary glands lumen Proventriculus PNS Neuroblasts Polarized Neuroblasts Midgut Midgut subset Hindgut Head Fat body Ectoderm Polarized Ectoderm CNS Ventral nerve cord Blastoderm nuclei Atrium Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around ectoderm nuclei Foci Small Foci Small Foci in tracheal cells Small foci in Proventriculus Small Foci in PNS Small Foci in hindgut Small Foci in head Small Foci in ectoderm Small Foci in CNS Cell junction associated

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing discs particular pattern Wing disc few cells - dots Expressed in eye disc Eye disc few cells - dots
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Ventral nerve cord - allover Localized to axons Expressed in brain lobes Brain lobes allover
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' Lymph Glands Expressed in lymph glands Lymph glands all over

Adult

Area	Images	Terms

Comments

Good probe on gel.

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions'

Probe SD09407 Links: Permalink

Genes: Dscam

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Gap-gene pattern Random blastoderm nuclei Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern PNS Oenocytes Midgut Head Ectoderm CNS Ventral nerve cord Foci - VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in PNS Small Foci in head Small Foci in ectoderm Small Foci in CNS Small Foci in Brain Basal enrichment Basal in midgut

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel. All over plus small foci.

Probe RE16656 Links: Permalink

Genes: EcR

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Degraded Degraded partially stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Salivary glands Salivary glands lumen Muscles-embryo Visceral mesoderm Midgut Ectoderm Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in other tissues Small Foci in midgut Small Foci in head Small Foci in ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Fat body - Few and small foci Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Expressed in midgut Subcellular localization in midgut Midgut - foci Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Hindgut allover Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - allover
salivary gland		3rd Instar Larval Tissues Salivary gland Expressed in salivary glands Salivary glands allover
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover

Adult

Area	Images	Terms

Comments

Good probe.

Probe LD11109 Links: Permalink

Genes: Ephrin

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Ubiquitous
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Pharynx Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Midgut Hindgut Head Fat body Ectoderm Brain Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Foci Small Foci Small Foci in Pharynx Small Foci in muscle cells Small Foci in midgut Small Foci in head Small Foci in ectoderm Small Foci in Brain

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Hindgut allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc few cells - dots Expressed in antennal disc Antennal disc few cells - dots Antennal disc - center round cells
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Nascent transcript in salivary gland Expressed in salivary glands Salivary glands allover
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms

Comments

Maybe; partly nascent, partly nuclear subregion.

Maybe, Partly nascent, partly nuclear subregion.

Good probe on gel. Not every embryo shows expression... maybe gender specific (?).

Not every embryo shows the same expression... maybe gender specific (?).

Probe Fili-RE Links: Permalink

Genes: Fili

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Zygotic Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Ubiquitous Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Ubiquitous Degraded Degraded partially stage 7
stage 8-9		Stage 8-9 Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Salivary glands Pharynx Muscles-embryo Maxillary complex Hindgut Specific hindgut area or cells Head Head epidermis Ectoderm Anal pad Ring around anal pad Ubiquitous Localized Subcellular localization patterns Perinuclear localization Perinuclear around salivary gland nuclei Perinuclear around muscle nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in head Small Foci in ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

-performed via HCR-FISH using 3 probe sets targeting the RE transcript isoform

-very neat germ band stage and late stage patterns; salivary gland pattern may be real

-images are from two separate experiments under identical conditions for better coverage

Probe CR34335 Links: Permalink

Genes: MRE16

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around all nuclei
stage 4-5		Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei All blastoderm nuclei Persistent widespread Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation in all nuclei Exclusionary patterns Apical exclusion Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Ventral yolk nuclei Anterior yolk nuclei Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern All blastoderm nuclei Persistent widespread Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Nuclei-associated localization Accumulation in subset nuclei
stage 10-17		Stage 10-17 Expressed Zygotic Midgut Hindgut Germ cell nuclei Gonads Foregut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Foci Small Foci Small Foci in head

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe SD03917 Links: Permalink

Genes: Neu3

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Gap-gene pattern Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Gap-gene pattern Expression in ectoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Apical exclusion Cytoplasmic foci Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts Expression in ectoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Head Ectoderm CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in head Small Foci in ectoderm

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules few foci Malpighian tubules allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms

Comments

Not all Nascent... Some nuclei have clearly one big dot (not two), sometimes right at the edge of the DAPI staining. Also, some cytoplasmic foci.

Good probe on gel.

Not all Nascent... Some nuclei have clearly one big dot (not two), sometimes right at the edge of the DAPI staining. Also, some cytoplasmic foci. Salivary glands, probably non-specific.

Probe SD05212 Links: Permalink

Genes: RhoL

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Exclusionary patterns Apical exclusion Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Cytoplasmic foci Embryonic localization patterns Anterior localization
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Segmented pattern Hemocytes Head Ectoderm Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in hemocytes Small Foci in head Small Foci in ectoderm Small Foci in amnioserosa

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe.

Plasmatocytes

Probe GM04578 Links: Permalink

Genes: Sgs4

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Salivary glands Salivary glands - specific region Muscles-embryo Muscles - subregion Head Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in salivary glands Small Foci in muscle cells Small Foci in head Small Foci in ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Sgs4

Good probe on gel.

Probe tll-E2 Links: Permalink

Genes: tll

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Segmented pattern Terminal-gene pattern Right flanking nuclei Left flanking nuclei
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Terminal-gene pattern Right flanking nuclei Left flanking nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal localization Basal enrichment Apical localization Apical enrichment Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Posterior yolk nuclei Pole cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Terminal-gene pattern Right flanking nuclei Left flanking nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Germ cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Anterior blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Nuclei-associated localization Cytoplasmic foci Germ cell localization Germ cell enrichment Embryonic localization patterns Anterior localization
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Tracheal system Tracheal lumen Salivary glands Salivary glands lumen Ring gland Midgut Hindgut Head Germ cell nuclei Gonads Brain Localized Subcellular localization patterns Nuclei-associated localization Nuclear in other cells Accumulation nuclear subregion Foci Small Foci Small Foci in head

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Fat body - Few and small foci Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus - Few foci Proventriculus allover Particular proventriculus area Expressed in midgut Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Hindgut allover Expressed in gastric caeca Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Wing disc few cells - dots Wing disc allover Expressed in leg discs Leg discs few cells - dots Leg discs allover Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc few cells - dots Eye disc allover Expressed in antennal disc
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Localized to axons Expressed in brain lobes Optic lobe Brain lobes - Significant area Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Perinuclear Salivary gland - Half moon perinuclear Salivary gland - Cytoplasmic foci Salivary gland - Small foci Expressed in salivary glands Salivary glands allover
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva Ovary allover Expressed in Ovary
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms

Comments

Home-made probe to exon 3. Good probe on gel.

Interesting patterns!

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions

Probe AT13267 Links: Permalink

Genes: W

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Posterior yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei All blastoderm nuclei Transiently widespread Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded completely stage 5
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Posterior blastoderm nuclei Expression in mesoderm Expression in ectoderm Expression in API Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in mesoderm Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Salivary glands Salivary glands lumen Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Hindgut Head Ectoderm CNS Ventral nerve cord Blastoderm nuclei Atrium Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Foci Small Foci Small Foci in head Small Foci in ectoderm Small Foci in CNS

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Interesting nervous system expression, including the ventral midline. Salivary glands, probably non-specific. Partly nascent, partly sub-nuclear.

Low probe on gel. Enough for a nice in situ...

Probe RE25924 Links: Permalink

Genes: ab

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Ubiquitous Localized Local terms for zygotic transcripts Embryonic localization patterns Ventral localization
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Posterior spiracles Specific posterior spiracle pattern PNS Muscles-embryo Head Ectoderm Polarized Ectoderm CNS Ventral nerve cord Foci - VNC Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in PNS Small Foci in other tissues Small Foci in muscle cells Small Foci in head Small Foci in ectoderm Small Foci in CNS

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe on gel - low. Nice in situ data!

Probe cid-RA Links: Permalink

Genes: cid

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk cortex localization Yolk cortex enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Degraded Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Expression in ectoderm Expression in API Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Germ band localization
stage 10-17		Stage 10-17 Expressed Zygotic Midgut Hindgut Head Head epidermis Ectoderm CNS Ventral nerve cord Axons Anal pad Localized Subcellular localization patterns Foci Small Foci Small Foci in hindgut Small Foci in head Small Foci in ectoderm Small Foci in CNS Small Foci in Brain

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

-performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform

-expression becomes weak in later stages but enriched foci are seen in several structures for a brief moment after dorsal retraction

Probe LD47466 Links: Permalink

Genes: dlp

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in ectoderm
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Segmented pattern Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Head Ectoderm Blastoderm nuclei Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in head Small Foci in ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

I wish we had more detailed photos... insitu done many years ago... re-annotated 2014 (rw)

Inside some nuclei.

Re-annotated 2014.

Probe RE04051 Links: Permalink

Genes: dpa

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Segmented pattern PNS Neuroblasts Midgut Hindgut Head Ectoderm Polarized Ectoderm CNS Ventral nerve cord Perinuclear VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around CNS nuclei Perinuclear 'rings' Foci Small Foci Small Foci in tracheal cells Small Foci in midgut Small Foci in hindgut Small Foci in head Small Foci in CNS Small Foci in Brain Apical enrichment Apical ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Allover with foci.

Good probe on gel.

Salivary glands, probably non-specific. Interesting patterns.

Probe RE20611 Links: Permalink

Genes: dpp

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical localization Apical enrichment Apical clusters Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Apical localization Apical enrichment Apical clusters Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Apical localization Apical enrichment Embryonic localization patterns
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Tracheal lumen Specific tracheal branches Dorsal trunk Neuroblasts Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Midgut Hindgut Head Ectoderm Blastoderm nuclei Atrium Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in other tissues Small Foci in midgut Small Foci in hindgut Small Foci in head Small Foci in ectoderm Small Foci in amnioserosa Apical enrichment Apical enriched hindgut

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Perinuclear localization Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Nascent transcript in proventriculus Expressed in midgut Subcellular localization in midgut Midgut - Nuclear Midgut - Subnuclear Nascent transcript in midgut Midgut allover Expressed in hindgut Hindgut - Midgut border Expressed in gastric caeca Gastric caeca allover
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area
ovary and testis		3rd Instar Larval Tissues Larval Ovary and Testis-Larva Ovary allover Expressed in Ovary
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva

Adult

Area	Images	Terms

Comments

Clear patches of cells close to the invaginating tracheal placodes.

Good probe on gel. RNA is allover, with some small but clear foci.

Probe GH20973 Links: Permalink

Genes: gol

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Non-Expressed Degraded Degraded completely stage 8 Degradation maternal transcripts
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands lumen Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Head Ectoderm CNS Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in head Small Foci in ectoderm Bigger Foci

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous Borderline between 'not expressed' and 'low expression'
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues Nervous system Not expressed in nervous system
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Muscles allover - Larva
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover

Adult

Area	Images	Terms

Comments

Good probe. Very little expression. Borderline between 'not expressed' and 'low expression'.

Small foci.

Salivary glands, probably non-specific.

Probe RE03728 Links: Permalink

Genes: kn

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Single stripe Expression in ectoderm Anterior blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Degraded Degraded partially stage 7 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Single stripe Expression in ectoderm Anterior blastoderm nuclei
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands lumen PNS Head Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Axons Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in PNS Nuclear in CNS Foci Small Foci Small Foci in PNS Small Foci in head Small Foci in CNS Small Foci in Brain Cell extensions or 'podia'

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Not every embryo has a pole cell excluded pattern.

Head, specific.

Good probe on gel. Small, localized foci.

Probe RE18093 Links: Permalink

Genes: msl-1

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous
stage 8-9		Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Hindgut Head Ectoderm Brain Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in hindgut Small Foci in head Small Foci in ectoderm Small Foci in Brain

Larva

Area	Images	Terms

Adult

Area	Images	Terms
Male reproductive tissues		Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatids -TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Early elongating spermatids-TE Cytoplasmic in early elongating spermatids Pigment cells -TE Nuclear in pigment cells -TE Muscle cells - TE Nuclear in muscle cells -TE Subcellularly localized - TE Distal tip region-TE Expressed in distal tip region - TE Lower expression in tip region Seminal Vesicle Subcellularly localized - SV Cell type - SV Cytoplasmic-SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Main cells -AG Cytoplasmic-MC Cytoplasmic Foci - AG

Comments

Very low probe on gel.

-missing some images? - stages missing are probably expressing ubiquitously and possibly in the early brain primordium expression (2024)

Probe SD07967 Links: Permalink

Genes: rhea

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in ectoderm Expression in amnioserosa Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Perinuclear around yolk nuclei Tracheal system Tracheal pits Segmented pattern Posterior spiracles Oenocytes Muscles-embryo Visceral mesoderm Midgut Ectoderm Dorsal vessel Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Foci Small Foci Small Foci in midgut Small Foci in head Small Foci in ectoderm Apical enrichment Apical ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Very, very low probe. Potential crosscontamination

-expression should be in the VNC but not seen here - contamination may be an issue - candidate for retesting (2025)

Probe rho-RB Links: Permalink

Genes: rho

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal Degraded Degraded partially stage 3 Degradation maternal transcripts
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Basal localization Apical localization
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in mesoderm Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Ventral localization Dorsal localization
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Single stripe Expression in ectoderm Anterior blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization Anterior localization
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Sensory system Posterior spiracles Muscles-embryo Visceral mesoderm Hindgut Head Head epidermis Dorsal vessel CNS Ventral nerve cord Foci - VNC Axons Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Foci Small Foci Small Foci in PNS Small Foci in head Small Foci in ectoderm Small Foci in CNS

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

-performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform

-neat and diverse expression patterns throughout development

-images are from two separate experiments under identical conditions for better coverage

Probe LD43989 Links: Permalink

Genes: scrib

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Posterior localization Other subcellular pattern Membrane associated Cytoplasmic foci Cell junction associated Anterior localization Embryonic localization patterns Yolk cortex localization Yolk cortex enrichment
stage 4-5		Stage 4-5 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Membrane associated Exclusionary patterns Pole cell exclusion Cell junction associated Basal localization Basal enrichment Apical localization Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Cell junction associated Basal localization Basal enrichment Apical localization Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Localized Subcellular localization patterns Perinuclear localization
stage 10-17		Stage 10-17 Expressed Zygotic Proventriculus Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Midgut Hindgut Hemocytes Ectoderm Polarized Ectoderm Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear around hemocytes Membrane associated Foci Small Foci Small Foci in head Small Foci in ectoderm Cell junction associated Apical enrichment Apical enriched hindgut Apical ectoderm

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Very interesting.

Salivary glands, probably non-specific.

Hemocytes-perinuclear, and visceral mesoderm

Good probe on gel. The RNA is localizaed outside - Amazing! It also has other particular paterns anterior and posterior.

Probe LP02726 Links: Permalink

Genes: sesB

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Non-Expressed Degraded Degraded completely stage 5
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Midgut Head CNS Ventral nerve cord Foci - VNC Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in midgut Small Foci in head Small Foci in CNS

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Very low probe on gel.

Probe stg-RA Links: Permalink

Genes: stg

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Ubiquitous Localized Subcellular localization patterns Yolk plasm localization Yolk cortex localization Nuclei-associated localization Intranuclear accumulation Exclusionary patterns Cytoplasmic foci Basal localization Basal enrichment Apical localization Apical enrichment Discrete apical foci Degraded Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Pair-rule pattern Expression in mesoderm Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei All blastoderm nuclei Transiently widespread Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Dorsal localization
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Expression in neuroblasts Expression in ectoderm Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Nuclei-associated localization Accumulation in subset nuclei Cytoplasmic foci Embryonic localization patterns Ventral localization Posterior localization Germ band localization Dorsal localization Anterior localization
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Optic lobes primordium Neuroblasts Maxillary complex Head Head epidermis Ectoderm Dorsal vessel CNS Ventral nerve cord Perinuclear VNC Nuclear - VNC Foci - VNC Brain Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in ectoderm Nuclear in CNS Foci Small Foci Small Foci in head Small Foci in ectoderm Small Foci in CNS Small Foci in Brain

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

-performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform

-very diverse and neat patterns at all stages

Probe LP02021 Links: Permalink

Genes: tun

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Right flanking nuclei Left flanking nuclei
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Right flanking nuclei Left flanking nuclei
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in API Expression in amnioserosa
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Midgut Head CNS Ventral nerve cord Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in head Small Foci in CNS Small Foci in amnioserosa

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Re-annoated by Ronit. Images from Eric's team.

Result Summary

Probe RE12073 Links: Permalink 3rd Instar Larval Tissues Annotated by Indrani Annotated by Ronit

Genes: CG46280

Embryo

Larva

Adult

Comments

Probe SD03066 Links: Permalink Annotated by Hideki Annotated by Ronit

Genes: CG13868

Embryo

Larva

Adult

Comments

Probe RE09732 Links: Permalink 3rd Instar Larval Tissues Annotated by Indrani Annotated by Ronit

Genes: CG14670

Embryo

Larva

Adult

Comments

Probe LD24702 Links: Permalink Annotated by Ronit

Genes: CG17286

Embryo

Larva

Adult

Comments

Probe LD47433 Links: Permalink Annotated by Hideki Annotated by Ronit

Genes: CG17724 Kdm4B

Embryo

Larva

Adult

Comments

Probe LD10058 Links: Permalink Annotated by Eric Annotated by Ronit

Genes: CG18431

Embryo

Larva

Adult

Comments

Probe RE06501 Links: Permalink Annotated by Ronit

Genes: CG31100

Embryo

Larva

Adult

Comments

Probe LD12677 Links: Permalink 3rd Instar Larval Tissues Annotated by Ronit

Genes: CG3136

Embryo

Larva

Adult

Comments

Probe AT21186 Links: Permalink Annotated by Ronit

Genes: CG32081

Embryo

Larva

Adult

Comments

Probe RE12054 Links: Permalink 3rd Instar Larval Tissues Annotated by Indrani Annotated by Ronit

Genes: koi

Embryo

Larva

Adult

Comments

Probe RE10255 Links: Permalink 3rd Instar Larval Tissues Annotated by Indrani Annotated by Ronit Annotated by Zhantao

Genes: CG33129

Embryo

Larva

Adult

Comments

Probe RE21703 Links: Permalink Annotated by Ronit

Genes: CG40198

Embryo

Larva

Adult

Comments

Probe RE27439 Links: Permalink Annotated by Ronit

Genes: CG6634

Embryo

Larva

Adult

Comments

Probe CG8289-RA Links: Permalink Annotated by Steven

Probe RE12073 Links: Permalink

Probe SD03066 Links: Permalink

Probe RE09732 Links: Permalink

Probe LD24702 Links: Permalink

Probe LD47433 Links: Permalink

Probe LD10058 Links: Permalink

Probe RE06501 Links: Permalink

Probe LD12677 Links: Permalink

Probe AT21186 Links: Permalink

Probe RE12054 Links: Permalink

Probe RE10255 Links: Permalink

Probe RE21703 Links: Permalink

Probe RE27439 Links: Permalink

Probe CG8289-RA Links: Permalink

Probe GM04053 Links: Permalink

Probe RE06388 Links: Permalink

Probe RE53348 Links: Permalink

Probe LD06565 Links: Permalink

Probe LD19584 Links: Permalink

Probe SD09407 Links: Permalink

Probe RE16656 Links: Permalink

Probe LD11109 Links: Permalink

Probe Fili-RE Links: Permalink

Probe CR34335 Links: Permalink

Probe SD03917 Links: Permalink