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| Number of genes | 47 | |
| Export | ||
| Good probe on gel. |
| Salivary glands, probably non-specific. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
| Interesting adult testis localization pattern. |
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| Tracheal placodes. |
| Re-annotated. |
| Good probe on gel. |
| Small foci |
| Salivary glands, probably non-specific. |
| Very mild enrichment in secondary cells - Adult Accessory Glands |
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| Nice in situ! |
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| Re-annotated by Ronit for stage 10-up. |
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| Good probe on gel. Clear, small foci. |
| One midgut chamber. |
| Some small foci. |
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| Good probe on gel. |
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| Salivary glands, probably non-specific. |
| Lots of specific expression, dynamic and strong. |
| Good probe in our gel. |
| Good probe on gel. |
| Good probe on gel. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -possibly the labral sense organ in the head |
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| Good probe on gel. |
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| Low probe on gel. |
| Nice patterns! |
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| -probes were designed against RA isoform |
| -tested using HCR-FISH probes (3 probe sets) |
| -appears ubiquitous with enrichment in ectoderm, CNS, and posterior spiracles |
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| Good probe on gel. |
| Good probe on gel. Allover with small foci. |
| Small foci (puncta). |
| Good probe on gel. |
| Nice foci. |
| Good probe on gel |
| Re-annotated |
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| Good probe on gel. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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| Good probe on gel. All over plus small foci. |
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| Good probe. |
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| Maybe; partly nascent, partly nuclear subregion. |
| Maybe; partly nascent, partly nuclear subregion. |
| Maybe, Partly nascent, partly nuclear subregion. |
| Good probe on gel. Not every embryo shows expression... maybe gender specific (?). |
| Not every embryo shows the same expression... maybe gender specific (?). |
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| -performed via HCR-FISH using 3 probe sets targeting the RE transcript isoform |
| -very neat germ band stage and late stage patterns; salivary gland pattern may be real |
| -images are from two separate experiments under identical conditions for better coverage |
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| -performed via HCR-FISH using 3 probe sets targeting the RC isoform transcript |
| -BDGP reported pericardial cell which was not observed in this experiment |
| -Expression seen in garland cells or proventriculus or both, difficult to distinguish between one or the other |
| -Faint expression seen in posterior and lateral epidermis |
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| -performed via HCR-FISH using 3 probe sets targeting the RI isoform transcript; difficult to pick best isoform to design probes to due to vast number of them, hard to say if these probes captured the complete expression profile for this gene |
| -Our previous experiments using TSA and independent experiments by BDGP observed maternal expression in stages 1-3 and stages 4-5, this was not observed in this experiment |
| -Interesting early mesoderm marker |
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| Not all Nascent... Some nuclei have clearly one big dot (not two), sometimes right at the edge of the DAPI staining. Also, some cytoplasmic foci. |
| Good probe on gel. |
| Not all Nascent... Some nuclei have clearly one big dot (not two), sometimes right at the edge of the DAPI staining. Also, some cytoplasmic foci. Salivary glands, probably non-specific. |
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| Good probe. |
| Plasmatocytes |
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| Sgs4 |
| Good probe on gel. |
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| Home-made probe to exon 3. Good probe on gel. |
| Interesting patterns! |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions |
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| Interesting nervous system expression, including the ventral midline. Salivary glands, probably non-specific. Partly nascent, partly sub-nuclear. |
| Low probe on gel. Enough for a nice in situ... |
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| Probe on gel - low. Nice in situ data! |
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| -performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
| -gorgeous epidermal patterns, also expressed in CNS, certain muscles, and gonads |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -expression becomes weak in later stages but enriched foci are seen in several structures for a brief moment after dorsal retraction |
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| I wish we had more detailed photos... insitu done many years ago... re-annotated 2014 (rw) |
| Inside some nuclei. |
| Re-annotated 2014. |
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| Allover with foci. |
| Good probe on gel. |
| Salivary glands, probably non-specific. Interesting patterns. |
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| Clear patches of cells close to the invaginating tracheal placodes. |
| Good probe on gel. RNA is allover, with some small but clear foci. |
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| Good probe. Very little expression. Borderline between 'not expressed' and 'low expression'. |
| Small foci. |
| Small foci. |
| Salivary glands, probably non-specific. |
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| Not every embryo has a pole cell excluded pattern. |
| Head, specific. |
| Good probe on gel. Small, localized foci. |
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| Very low probe on gel. |
| -missing some images? - stages missing are probably expressing ubiquitously and possibly in the early brain primordium expression (2024) |
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| Very, very low probe. Potential crosscontamination |
| -expression should be in the VNC but not seen here - contamination may be an issue - candidate for retesting (2025) |
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| -performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
| -neat and diverse expression patterns throughout development |
| -images are from two separate experiments under identical conditions for better coverage |
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| Very interesting. |
| Salivary glands, probably non-specific. |
| Hemocytes-perinuclear, and visceral mesoderm |
| Good probe on gel. The RNA is localizaed outside - Amazing! It also has other particular paterns anterior and posterior. |
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| Very low probe on gel. |
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| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -very diverse and neat patterns at all stages |
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| Re-annoated by Ronit. Images from Eric's team. |