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Result Summary

Number of genes	27
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Probe LP03571 Links: Permalink

Genes: CG13397

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Exclusionary patterns Apical exclusion Basal localization Basal enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Perinuclear around yolk nuclei Inside Yolk nuclei Tracheal system Specific tracheal branches Salivary glands Midgut Dorsal vessel Dorsal vessel specific pattern Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Foci Small Foci Small Foci in ectoderm Small Foci in dorsal vessel Small Foci in Brain

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel.

Nice yolk!

Probe RE20371 Links: Permalink

Genes: CG13912

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Weak expression (>41ms exp)
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in API Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Nuclei-associated localization Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Perinuclear around yolk nuclei Tracheal system Specific tracheal branches Hindgut Ectoderm Dorsal vessel Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around ectoderm nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in dorsal vessel Basal enrichment Basal in hindgut

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Hindgut allover Hindgut - Midgut border Expressed in gastric caeca Gastric caeca allover
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover

Adult

Area	Images	Terms

Comments

Good probe on gel.

We did not catch the nascent transcript on these images.

Probe LD10058 Links: Permalink

Genes: CG18431

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Non-Expressed
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Expressed Zygotic Zygotic starting stage 9 Blastoderm nuclei Subset blastoderm nuclei Expression in API Anterior blastoderm nuclei
stage 10-17		Stage 10-17 Expressed Zygotic Salivary glands Posterior spiracles Specific posterior spiracle pattern Head Ectoderm Dorsal vessel Dorsal vessel specific pattern Blastoderm nuclei Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in head Small Foci in dorsal vessel

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Re-annotated by Ronit for stage 10-up.

Probe RE29404 Links: Permalink

Genes: Nlg1

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Exclusionary patterns Apical exclusion Cytoplasmic foci Basal localization Basal enrichment
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Segmented pattern Posterior spiracles Muscles-embryo Hindgut Specific hindgut area or cells Ectoderm Dorsal vessel Dorsal vessel specific pattern Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in muscle cells Small Foci in ectoderm Small Foci in dorsal vessel Basal enrichment Basal in hindgut

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Nascent transcript in fat body Expressed in fat body Fat body allover - Ubiquitous
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs particular pattern Expressed in eye disc Morphogenetic furrow Expressed in antennal disc Antennal disc few cells - dots
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Foci or dots
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Nuclear Localization in larval muscles Sub-nuclear localization larval muscles Larval Muscles - Cytoplasmic foci Foci in larval muscles edge Nascent in larval muscle cells -Larva
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms

Comments

Good probe on gel

Nice patterns

Probe LP08452 Links: Permalink

Genes: CG32046

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Cytoplasmic foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern PNS Dorsal vessel CNS Ventral nerve cord Polarized VNC Foci - VNC Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around PNS nuclei Perinuclear around dorsal vessel nuclei Foci Small Foci Small Foci in PNS Small Foci in dorsal vessel Small Foci in CNS

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Interesting.

Low low probe on gel. Nice small foci.

Small foci

Small foci.

Probe RE56803 Links: Permalink

Genes: CG32714

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Non-Expressed Degraded Degraded completely stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Non-Expressed
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'podia' Yolk nuclei 'foci' Perinuclear around yolk nuclei Dorsal vessel Localized Subcellular localization patterns Perinuclear localization Foci Small Foci Small Foci in dorsal vessel Cell extensions or 'podia'

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe. Gene withdrawn in fly base... only a few photos taken.

Probe AT18914 Links: Permalink

Genes: CG33130

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal
stage 4-5		Stage 4-5 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Membrane associated Exclusionary patterns Pole cell exclusion Cytoplasmic foci Cell junction associated Cell division apparatus Basal localization Basal enrichment Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Membrane associated Exclusionary patterns Cytoplasmic foci Cell junction associated Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Localized Subcellular localization patterns Membrane associated Cytoplasmic foci Cell junction associated
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Inside Yolk nuclei Segmented pattern Salivary glands Salivary glands lumen Muscles-embryo Muscles - subregion Head Ectoderm Dorsal vessel Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in other tissues Small Foci in ectoderm Small Foci in dorsal vessel

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel.

Salivary glands, probably non-specific. Small Foci

Probe GH23906 Links: Permalink

Genes: CG3960

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Pharynx Muscles-embryo Visceral mesoderm Hindgut Dorsal vessel Blastoderm nuclei Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in Pharynx Small Foci in muscle cells Small Foci in dorsal vessel Bigger Foci Basal enrichment Basal in hindgut

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Low probe... Still, nice insitu. All over with some small foci.

Small foci.

Probe RE24423 Links: Permalink

Genes: CG4576

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Embryonic localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Yolk cortex exclusion
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Cytoplasmic foci Cell junction associated Apical localization Apical enrichment Discrete apical foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Yolk plasm localization Yolk plasm enrichment Subcellular localization patterns Exclusionary patterns Apical exclusion Basal localization Basal enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Expression in amnioserosa Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Sensory system Segmented pattern Muscles-embryo Hindgut Specific hindgut area or cells Ectoderm Polarized Ectoderm Dorsal vessel Brain Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around muscle nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in muscle cells Small Foci in hindgut Small Foci in ectoderm Small Foci in dorsal vessel Small Foci in Brain Apical enrichment Apical enriched hindgut

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in hindgut Hindgut allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc few cells - dots Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc basal Expressed in antennal disc Antennal disc few cells - dots
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - Cytoplasmic foci Expressed in salivary glands Salivary glands allover
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression'

Adult

Area	Images	Terms

Comments

Good probe.

It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions'

Probe LP05865 Links: Permalink

Genes: CG5656

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Maternal
stage 4-5		Stage 4-5 Non-Expressed
stage 6-7		Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Segmented pattern Salivary glands PNS Head Ectoderm Dorsal vessel Dorsal vessel specific pattern Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around ectoderm nuclei Perinuclear around dorsal vessel nuclei Nuclei-associated localization Nuclear in ectoderm Accumulation nuclear subregion Foci Small Foci Small Foci in ectoderm Small Foci in dorsal vessel Cell junction associated

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Very low expression.

Good probe on gel. Very low expression.

Partly nascent, partly sub-nuclear.

Probe LP06937 Links: Permalink

Genes: CG7702

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Non-Expressed Degraded Degraded completely stage 4 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Ubiquitous
stage 8-9		Stage 8-9 Expressed Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Perinuclear around yolk nuclei Tracheal system Tracheal lumen Specific tracheal branches Sensory system Salivary glands Salivary glands lumen Proventriculus PNS Muscles-embryo Muscles - subregion Midgut Midgut subset Midgut ICPs Malpighian tubules Hindgut Dorsal vessel Dorsal vessel specific pattern Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Foci Small Foci Small Foci in dorsal vessel Cell extensions or 'podia'

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe. Low expression.

Probe AT29333 Links: Permalink

Genes: CG8486

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Gap-gene pattern Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Single stripe Segmented pattern Gap-gene pattern Expression in ectoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Expression in neuroblasts Expression in ectoderm Expression in amnioserosa
stage 10-17		Stage 10-17 Expressed Zygotic Posterior spiracles Midgut Head Ectoderm Polarized Ectoderm Dorsal vessel Dorsal vessel specific pattern Brain Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus Foci Small Foci Small Foci in dorsal vessel

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel.

Brain, optic lobe, ventral nerve cord, head epidermis.

Epidermis, dorsal vessel.

Not at all stages it is 'Apical excluded' Nice Gap expression!

Probe SD02996 Links: Permalink

Genes: CG8557

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Exclusionary patterns Apical exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Expression in neuroblasts Weak expression (>41ms exp)
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Segmented pattern Salivary glands Salivary glands lumen Proventriculus Pharynx Midgut Hindgut Specific hindgut area or cells Ectoderm Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Brain Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in Pharynx Small Foci in dorsal vessel Small Foci in CNS Basal enrichment Basal in hindgut

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel. All over with small foci.

Probe LD19219 Links: Permalink

Genes: Sep4

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization RNA islands Pole plasm Pole buds Embryonic localization patterns
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Exclusionary patterns Apical exclusion Cytoplasmic foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci Pole cell localization Pole cell enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Salivary glands Salivary glands lumen Posterior spiracles Muscles-embryo Visceral mesoderm Hindgut Specific hindgut area or cells Ectoderm Dorsal vessel CNS Blastoderm nuclei Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in hindgut Small Foci in ectoderm Small Foci in dorsal vessel Small Foci in CNS Apical enrichment Apical enriched hindgut

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Particular proventriculus area Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules few foci Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca foci Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area Ventral nerve cord - foci or 'dots'
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands Borderline between 'not expressed' and 'low expression'
ovary and testis		Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Testis allover - Larva Specific testis expression localization Larval testis foci Testis foci outside-Larva
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands

Adult

Area	Images	Terms

Comments

Good, strong probe on gel.

allover with some 'foci' or 'puncta'

Probe LD25556 Links: Permalink

Genes: Fas1

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Expression in ectoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Sensory system Segmented pattern PNS Head Ectoderm Dorsal vessel CNS Ventral nerve cord Polarized VNC Axons Brain Blastoderm nuclei Atrium Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around PNS nuclei Perinuclear around dorsal vessel nuclei Perinuclear around CNS nuclei Nuclei-associated localization Nuclear in CNS Accumulation nuclear subregion Foci Small Foci Small Foci in PNS Small Foci in dorsal vessel Small Foci in CNS Cell extensions or 'podia'

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel Reannotated.

Leading edge, ectoderm.

Probe RE24732 Links: Permalink

Genes: GluRIID

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci Embryonic localization patterns Exclusionary patterns Pole plasm excluded
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Cytoplasmic foci Apical localization Apical enrichment Discrete apical foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Yolk plasm localization Yolk plasm enrichment Subcellular localization patterns Exclusionary patterns Apical exclusion Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Pharynx Muscles-embryo Muscles - subregion Hindgut Specific hindgut area or cells Foregut Ectoderm Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around dorsal vessel nuclei Foci Small Foci Small Foci in Pharynx Small Foci in hindgut Small Foci in ectoderm Small Foci in dorsal vessel Small Foci in CNS Basal enrichment Basal in hindgut

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
imaginal discs		3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Wing disc few cells - dots Expressed in leg discs Leg discs few cells - dots
nervous system		3rd Instar Larval Tissues Nervous system Not expressed in nervous system
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Localized to larval muscle band Larval Muscles - Cytoplasmic foci Expressed in heart
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands Lymph glands all over

Adult

Area	Images	Terms

Comments

Foci outside, or inside, or peri-nuclear.

Good probe on gel, a bit weak. Nice in situ.

Probe LD23292 Links: Permalink

Genes: Mcr

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Specific tracheal branches Posterior spiracles Pharynx Hindgut Ectoderm Dorsal vessel CNS Ventral nerve cord Perinuclear VNC Foci - VNC Axons Brain Blastoderm nuclei Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in hindgut Small Foci in ectoderm Small Foci in dorsal vessel Small Foci in CNS Cell extensions or 'podia' Basal enrichment Basal (outside) in brain

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Partly nascent, partly sub-nuclear.

Small foci.

Very low probe on gel (?). Nice in situ

Probe RE21674 Links: Permalink

Genes: Nhe2

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Cytoplasmic foci Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Ubiquitous
stage 8-9		Stage 8-9 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Muscles-embryo Muscles - subregion Midgut Head Fat body Fat body- specific region Ectoderm Dorsal vessel Dorsal vessel specific pattern Blastoderm nuclei Atrium Localized Subcellular localization patterns Foci Small Foci Small Foci in muscle cells Small Foci in ectoderm Small Foci in dorsal vessel

Larva

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Expressed in hindgut Subcellular localization in hindgut Nuclear in hindgut Hindgut - subnuclear localization Hindgut perinuclear Hindgut membranes Particular hindgut area Nascent transcript in hindgut Hindgut allover
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area Brain lobes - Foci or dots
salivary gland		3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - Perinuclear Salivary gland - nuclear dots Salivary gland - Cytoplasmic foci Salivary gland - Small foci Nascent transcript in salivary gland Expressed in salivary glands Salivary glands allover
muscles		3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Nascent transcript in ring gland Expressed in ring gland Ring gland allover Lymph Glands Expressed in lymph glands Lymph glands all over

Adult

Area	Images	Terms

Comments

Small foci.

Good probe on gel.

Probe LD22004 Links: Permalink

Genes: Nrt

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Ventral localization Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Dorsal localization Cell division apparatus Other cell division pattern Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Other subcellular pattern Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Cell division apparatus Apical localization Apical enrichment Discrete apical foci Apical clusters Embryonic localization patterns Ventral localization Exclusionary patterns Pole cell exclusion Dorsal localization
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in neuroblasts Expression in amnioserosa Weak expression (>41ms exp) Localized Subcellular localization patterns Perinuclear localization Nuclei-associated localization Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Apical Localization Neuroblasts Embryonic localization patterns Ventral localization Dorsal localization
stage 10-17		Stage 10-17 Expressed Zygotic PNS Fat body Fat body- specific region Fat body polarized Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Polarized VNC Perinuclear VNC Polarized CNS Brain Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around fat body nuclei Perinuclear around CNS nuclei Membrane associated Foci Small Foci Small Foci in fat body Small Foci in dorsal vessel

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe LD08230 Links: Permalink

Genes: Sdc

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Intranuclear accumulation Accumulation nuclear subregion Membrane associated Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Ventral localization
stage 8-9		Stage 8-9 Expressed Zygotic Weak expression (>41ms exp) Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal pits Tracheal lumen Specific tracheal branches Segmented pattern Salivary glands Posterior spiracles Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Hindgut Specific hindgut area or cells Ectoderm Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Blastoderm nuclei Amnioserosa Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in ectoderm Small Foci in dorsal vessel Small Foci in amnioserosa Apical enrichment Apical enriched hindgut

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Salivary glands, probably non-specific. High in yolk.

Important note: these images are from a home made probe for the Sdc gene.

Probe LD45234 Links: Permalink

Genes: Spn

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Other subcellular pattern Nuclei associated localization Perinuclear localization Perinuclear around cortical nuclei Membrane associated Cell division apparatus Other cell division pattern Embryonic localization patterns Yolk cortex localization Yolk cortex enrichment
stage 4-5		Stage 4-5 Expressed Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Posterior localization Pole cell localization Pole cell enrichment Other subcellular pattern Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Membrane associated Exclusionary patterns Pole cell exclusion Cytoplasmic foci Cell junction associated Basal localization Basal enrichment Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Membrane associated Cytoplasmic foci Cell junction associated Basal localization Basal enrichment Embryonic localization patterns
stage 8-9		Stage 8-9 Expressed Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Inside Yolk nuclei Tracheal system Tracheal lumen Posterior spiracles Specific posterior spiracle pattern PNS Pharynx Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Ectoderm Dorsal vessel Dorsal vessel specific pattern CNS Brain Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in tracheal cells Small Foci in PNS Small Foci in dorsal vessel Bigger Foci

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Salivary glands, probably non-specific.

Very interesting! Enriched posterior, not in the pole cells and with some basal sub-nuclear acumulation in posterior epithelia and in budding pole cells.

Good probe on gel. Clear localization to the outside of the embryo with interesting patterns.

Probe RE17794 Links: Permalink

Genes: beat-IIa

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 6-7		Stage 6-7 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 8-9		Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Polarized tracheal localization Sensory system Salivary glands Salivary glands lumen PNS Pharynx Muscles-embryo Visceral mesoderm Specific visceral mesoderm pattern Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Polarized VNC Foci - VNC Axons Blastoderm nuclei Amnioserosa Amnioserosa edges Localized Subcellular localization patterns Perinuclear localization Perinuclear around amnioserosa nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in PNS Small Foci in dorsal vessel Small Foci in CNS Small Foci in amnioserosa Cell junction associated Cell extensions or 'podia'

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel.

Salivary glands, probably non-specific.

Probe eve-RA Links: Permalink

Genes: eve

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Non-Expressed
stage 4-5		Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Pair-rule pattern Localized Subcellular localization patterns Degraded Degraded partially stage 5 Degraded partially stage 4
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Degraded Degraded partially stage 7 Degraded partially stage 6
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Dorsal blastoderm nuclei Localized Embryonic localization patterns Degraded Degraded partially stage 8
stage 10-17		Stage 10-17 Expressed Zygotic Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Foci - VNC Anal pad Ring around anal pad Localized Subcellular localization patterns Foci Small Foci Small Foci in dorsal vessel

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

-probes were designed against eve-RA isoform transcript

-tested using HCR-FISH probes (3 probe sets)

-very neat stripe patterns

Probe LD24308 Links: Permalink

Genes: l(1)G0193

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Basal localization Basal enrichment Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 7 Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment
stage 8-9		Stage 8-9 Expressed Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Hindgut Ectoderm Dorsal vessel Dorsal vessel specific pattern Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in dorsal vessel

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Good probe on gel.

Probe GH08636 Links: Permalink

Genes: phtf

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment Pole cell localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Ectoderm Dorsal vessel Dorsal vessel specific pattern Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in ectoderm Small Foci in dorsal vessel

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

Probe RE01329 Links: Permalink

Genes: tin

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Degraded Degraded partially stage 4 Degraded completely stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Weak expression (>41ms exp) Localized
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Ectoderm Polarized Ectoderm Dorsal vessel Dorsal vessel specific pattern Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around ectoderm nuclei Perinuclear around dorsal vessel nuclei Foci Small Foci Small Foci in dorsal vessel

Larva

Area	Images	Terms

Adult

Area	Images	Terms

Comments

These images are from a home-made probe and not from probe 'RE01329'. Same CG number (reports for tin)' More images added from probe 'RE01329' (same data).

Borderline between 'not expressed' and 'low expression'.

Expressed at the dorsal mesoderm.

Probe GH12341 Links: Permalink

Genes: tup

Embryo

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Cytoplasmic foci
stage 4-5		Stage 4-5 Expressed Zygotic Zygotic starting stage 5 Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Dorsal blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts
stage 6-7		Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Dorsal blastoderm nuclei Weak expression (>41ms exp) Localized
stage 8-9		Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in API Expression in amnioserosa Dorsal blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Sensory system Salivary glands Proventriculus Muscles-embryo Muscles - subregion Head Foregut Esophagus Ectoderm Dorsal vessel Dorsal vessel specific pattern CNS Ventral nerve cord Axons Brain Blastoderm nuclei Atrium Amnioserosa Localized Subcellular localization patterns Perinuclear localization Perinuclear around CNS nuclei Other subcellular pattern Foci Small Foci Small Foci in Pharynx Small Foci in muscle cells Small Foci in ectoderm Small Foci in dorsal vessel Small Foci in CNS Cell extensions or 'podia'