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Number of genes | 47 | |
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Good probe. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions |
Sometimes one larval brain lobe reacts to the in situ hybridization and the other one does not - it may be that one of the reagents was not exposed properly - This in situ may have to be redone in larval brains for absolute confirmation. |
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Good probe on gel. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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Low probe on gel. Beautiful neuronal pattern in embryos! |
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Bright probe on gel. Small foci. |
Small foci. |
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Re-annotated in 2014. Insitu done originally in 2006. |
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Probe looks good on gel. Maybe some small foci... |
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Good probe. |
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Good probe on gel. |
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Salivary glands, probably non-specific. Perinuclear. |
Good probe on gel. |
Not excluded apically in all embryos. |
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Good probe |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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Good probe, a bit weak. |
Borderline between 'not expressed' and 'low expression' |
Salivary glands, probably non-specific. |
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Not sure if this is 'specific' expression... |
Nice nervous system. Salivary glands, probably non-specific. |
Low probe on gel (the late stage expression shows that the probe works fine) |
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Interesting. |
Low low probe on gel. Nice small foci. |
Small foci |
Small foci. |
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Good probe. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
Good probe on gel. |
Comet localization in adult testes. |
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Good probe. |
Perinuclear. |
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Lowish probe on gel. |
It degrades and at the same time the nascent starts. |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-expression appears quite ubiquitous, but closer examination reveals enrichment in CNS and head sensory (maybe antennal-maxillary complex?) and trunk PNS |
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Good probe on gel. |
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Good probe on gel. |
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Good probe on gel |
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-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript |
-quite unique expression pattern that appear as highly localized and nuclear small foci along a strip of the early blastoderm; and as small highly localized foci in the early anterior-dorsal head, API, ventral cephalic furrow; and lastly in ectodermal regions of late stages representing PNS patterns |
-images are from two separate experiments using different hairpin-fluorophores for signal amplification but otherwise identical conditions |
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-performed via HCR-FISH using 3 probe sets targeting the RC isoform transcript |
-strongly ubiquitous at late stages with enrichment in the posterior anal pads and an anterior-lateral sub-region of near the brain |
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Good probe on gel. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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Good probe on gel. All over plus small foci. |
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Tracheal lumen, probably non-specific. |
Good probe. |
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Good probe on gel Reannotated. |
Leading edge, ectoderm. |
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Borderline between 'not expressed' and 'low expression' |
Borderline between 'not expressed' and 'low expression' |
Good probe. |
Specific tracheal areas and branches. |
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Salivary glands, probably non-specific. |
Low probe on gel. Good in situ. |
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Tracheal lumen, probably non-specific. |
Good strong probe. |
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Re-annotated |
Atrium membrane |
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Salivary glands, probably non-specific. |
Very interesting! Enriched posterior, not in the pole cells and with some basal sub-nuclear acumulation in posterior epithelia and in budding pole cells. |
Good probe on gel. Clear localization to the outside of the embryo with interesting patterns. |
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Interesting patterns! |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
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Probe on gel - low. Nice in situ data! |
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Good probe on gel. |
Salivary glands, probably non-specific. |
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Good probe. We use it as one positive control for the screen. |
Salivary glands, probably non-specific. |
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Good probe on gel. |
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-detected using HCR-FISH protocol (3 probe sets) |
-probes were designed against dap-RA isoform transcript |
-really neat expression patterns |
-images are from two separate experiments under identical conditions for better coverage |
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Nuclear localization and other patterns. |
Good probe on gel. |
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Interesting |
Low probe on gel. |
Interesting patterns. Salivary glands, maybe non-specific. |
Interesting |
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Not every embryo has a pole cell excluded pattern. |
Head, specific. |
Good probe on gel. Small, localized foci. |
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Good probe on gel. Small clear foci. |
Few- but clear, foci. |
Interesting. |
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-performed via HCR-FISH using 3 probe sets targeting the RS isoform transcript (this particular gene has 60 recorded isoforms on FlyBase - further optimization of transcript probe targeting and possibly more probe sets should be considered if a stronger signal is desired) |
-weak but highly dynamic PNS expression patterns, possibly can be better captured by increasing the probe sets |
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-performed via HCR-FISH using 3 probe sets targeting the RB transcript isoform |
-neat and diverse expression patterns throughout development |
-images are from two separate experiments under identical conditions for better coverage |
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Good probe on gel. |