Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Zygotic    Zygotic starting stage 1   Weak expression (>41ms exp)   Maternal   Localized    Subcellular localization patterns     Nuclei associated localization      Pronucleus-associated      Perinuclear localization       Perinuclear around all nuclei      Intranuclear accumulation       Accumulation in all nuclei     Cell division apparatus      Chromatin associated metaphase    Local terms for zygotic transcripts
stage 4-5		Stage 4-5  Expressed   Zygotic    Zygotic starting stage 4    Yolk nuclei     All yolk nuclei    Pole cell nuclei     Subset pole cell nuclei    Blastoderm nuclei     All blastoderm nuclei   Medium expression (21-40ms exp)   Localized    Subcellular localization patterns     Pole cell localization      Pole cell enrichment     Nuclei-associated localization      Perinuclear localization       Perinuclear subset of nuclei        Perinuclear around pole cell nuclei      Intranuclear accumulation       Accumulation nuclear subregion     Cytoplasmic foci     Cell division apparatus      Chromatin associated metaphase    Local terms for zygotic transcripts
stage 6-7		Stage 6-7  Expressed   Zygotic    Yolk Nuclei     All yolk nuclei    Pole cell nuclei     Subset pole cell nuclei    Blastoderm nuclei     All blastoderm nuclei   Medium expression (21-40ms exp)   Localized    Subcellular localization patterns     Nuclei-associated localization      Perinuclear localization       Perinuclear subset of nuclei        Perinuclear around yolk nuclei      Intranuclear accumulation       Accumulation nuclear subregion     Cell division apparatus      Chromatin associated metaphase    Local terms for zygotic transcripts
stage 8-9		Stage 8-9  Expressed   Zygotic    Yolk nuclei     All yolk nuclei    Blastoderm nuclei     All blastoderm nuclei   Localized    Subcellular localization patterns     Perinuclear localization      Perinuclear around yolk nuclei     Nuclei-associated localization      Intranuclear accumulation      Accumulation nuclear subregion     Apical localization    Local terms for zygotic transcripts
stage 10-17		Stage 10-17  Expressed   Zygotic    Yolk nuclei     Inside Yolk nuclei    Tracheal system     Specific tracheal branches    Segmented pattern    Salivary glands    Posterior spiracles     Specific posterior spiracle pattern    Midgut    Head     Head epidermis    Ectoderm    Atrium   Localized    Subcellular localization patterns     Nuclei-associated localization      Accumulation nuclear subregion     Foci      Small Foci       Small Foci in tracheal cells       Small Foci in other tissues       Small Foci in ectoderm

Area	Images	Terms
fat body		3rd Instar Larval Tissues  Fat body   Subcellular Localization - Fat body    Fat body - Nuclear localization     Fat body - Subnuclear localization   Nascent transcript in fat body   Expressed in fat body
intestine		3rd Instar Larval Tissues  Intestine   Expressed in proventriculus    Proventriculus allover    Particular proventriculus area   Expressed in midgut    Subcellular localization in midgut     Midgut - foci   Expressed in malpighian tubules    Subcellular localization in malpighian tubules     Malpighian tubules - Nuclear in      Malpighian tubules - Subnuclear in    Nascent transcript in malpighian tubules    Malpighian tubules allover   Expressed in hindgut    Subcellular localization in hindgut     Nuclear in hindgut      Hindgut - subnuclear localization     Hindgut foci    Nascent transcript in hindgut    Hindgut allover   Expressed in gastric caeca    Subcellular localization in gastric caeca     Gastric caeca - nuclear localization      Gastric caeca - subnuclear localization    Nascent transcript in gastric caeca    Gastric caeca allover
imaginal discs		3rd Instar Larval Tissues  Imaginal discs   Expressed in leg discs    Leg discs particular pattern    Leg discs few cells - dots   Expressed in eye disc    Morphogenetic furrow    Eye disc photo-receptors    Eye disc few cells - dots    Eye disc - center round cells    Expressed in antennal disc
nervous system		3rd Instar Larval Tissues  Nervous system   Expressed in ventral nerve cord    Ventral nerve cord - particular area    Ventral nerve cord - foci or 'dots'   Expressed in brain lobes    Optic lobe    Brain lobes - Significant area
salivary gland		3rd Instar Larval Tissues  Salivary gland   Subcellular localization in salivary gland    Salivary gland - subnuclear    Salivary gland - nuclear dots    Salivary gland - Cytoplasmic foci     Cytoplasmic foci in Salivary Gland Lumen    Salivary gland - apical     Salivary gland - lumen   Nascent transcript in salivary gland   Expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues  Lymph Glands and Ring Glands   Ring gland    Ring gland - Subcellular localization     Ring gland - Nuclear      Ring gland - Subnuclear    Nascent transcript in ring gland    Expressed in ring gland

Area	Images	Terms
Male reproductive tissues		Adult Tissues  Male reproductive tissues   Testis    Testis Cell type and stage - TE     Spermatocytes -TE      Y-Loop pattern - TE      Nuclear in Spermatocytes - TE      Cytoplasmic in Spermatocytes - TE     Spermatids -TE      Round spermatids-TE       Nuclear in round spermatids-TE       Cytoplasmic in round spermatids-TE      Elongating Spermatids - TE       Cytoplasmic in elongating spermatids-TE     Cyst cells -TE      Tail cyst cell - TE       Sperm Tail Decoration-TCC associated        Retracting/Coiling sperm tail-TCC associated       Retracting/Coiling Tail Cyst Cell - TE        Retracting/Coiling Tail Cyst Cell Cytoplasm - TE    Subcellularly localized - TE    Nuclear    Expression level-TE     Expressed Medium - TE    Distal tip region-TE     Expressed in distal tip region - TE      Specific tip region cells-TE       Early cyst cell        Cytoplasmic in early cyst cell      Lower expression in tip region    Cytoplasmic   Seminal Vesicle    Subcellularly localized - SV    Lumenal-SV     Scattered Foci - SV     ECM like pattern-SV    Expression level-SV     Expressed Medium - SV    Cell type - SV     Sperm Tail Decor - SV     Cytoplasmic-SV      Cytoplasmic Foci - SV   Ejaculatory Duct    Subcellularly localized - ED    Expression level-ED     Expressed Low - ED    Cell type - ED     Nuclear-ED      Nuclear associated - Ejaculatory Duct       Subnuclear - Ejaculatory Duct        Nuclear Foci - Ejaculatory Duct     Cytoplasmic-ED      Membrane enriched - ED      Cytoplasmic Foci - ED   Accessory Gland    Subcellularly localized - AG    Lumenal - AG     Non-particulate - AG    Expression level-AG     Expressed Medium - AG    Cytoplasmic    Cell type - AG     Secondary cells - AG      Nuclear-SC       Nuclear Foci-SC      Cytoplasmic-SC       Multivesicular bodies - SC     Main cells -AG      Nuclear- MC       Nuclear foci-mc      Cytoplasmic-MC       Cytoplasmic Foci - AG

The probe may be recognizing 16 transcripts - Including flamenco. The probe may recognize some other areas in the genome that contain a highly homologous sequence.

Amazing patterns!

Very interesting patterns in adult testes and associated tissues.

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Zygotic    Zygotic starting stage 1    Yolk nuclei     All yolk nuclei    Cortical nuclei     All cortical nuclei   Weak expression (>41ms exp)   Localized    Local terms for zygotic transcripts
stage 4-5		Stage 4-5  Expressed   Zygotic    Yolk nuclei     All yolk nuclei    Pole cell nuclei     All pole cell nuclei    Blastoderm nuclei     All blastoderm nuclei   Weak expression (>41ms exp)   Localized    Subcellular localization patterns     Cell division apparatus      Chromatin associated metaphase    Local terms for zygotic transcripts
stage 6-7		Stage 6-7  Expressed   Zygotic    Yolk Nuclei     All yolk nuclei    Pole cell nuclei     All pole cell nuclei    Blastoderm nuclei     All blastoderm nuclei   Weak expression (>41ms exp)
stage 8-9		Stage 8-9  Expressed   Zygotic    Yolk nuclei     All yolk nuclei    Blastoderm nuclei     All blastoderm nuclei   Weak expression (>41ms exp)

Area	Images	Terms

Area	Images	Terms

84 ms I think it is all zygotic, nothing sub-cellular (?). I wish we had 40x images.

84 ms

84 ms

84 ms Re-annotated by Ronit- 2014.