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Number of genes | 27 | |
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Note: need to redo this experiment to confirm patterns ! |
Good probe on gel. |
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Expression is maintained in germ cells following gastrulation. |
RNA localization is very dynamic, ranging from perinuclear, to apical, and finally pole cell enrichment. |
RNA exhibits perinuclear localization syggesting association with microtubules. |
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Good probe on gel. |
Small foci. |
Nice CNS. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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Good probe on gel. |
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36 ms |
36 ms |
36 ms |
36 ms |
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Re-annotated. insitu done in 2006 by Eric's group. |
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Cytoplasmic foci around the pole cells. |
Good probe on gel (a bit low) |
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Salivary glands and tracheal lumen, probably non-specific |
Good probe, good plate. |
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Re-annotated |
Need more images to get the full story. |
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Re-annotated |
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Low probe on gel. Nice insitu. Interesting patterns of localization. |
Asymmetric perinuclear localization in pole cells. |
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Allover, enriched in pole cells. Includes apical diffuse, and small 'foci' or 'puncta'. |
Good probe on gel. |
Salivary glands, probably non-specific. |
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Tracheal lumen, probably non-specific. |
Good probe. |
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Cytoplasmic foci around pole cells. |
Pole plasm and pole buds enriched. |
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Re-annotated. Original insitu done by Eric's group. |
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Re-annotated |
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insitu done in 2006 by Eric's group. |
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The probe is good but low' Good insitu ------ Intersting expression. |
Partly nascent, partly sub-nuclear. |
Partly nascent, partly sub-nuclear. |
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Salivary glands, probably non-specific. |
Good probe on gel ovo transcript on X. |
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Again, perinuclear pole cell asymmetric expression. |
Pole cell asymmetric expression. I see small 'dots' around the pole cells, stronger in one 1/2 of the pole nuclei. |
Good probe on gel |
Posterior localization, even in unfertilized eggs. RNA accumulates asymmetrically around the pole buds with a 'fiber-like' texture. When nuclei divide, the RNA will stay in the nuclei that are closer to the posterior end. Very interesting! |
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Good probe Very interesting localization! |
Nice! |
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Low, low probe. |