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| Number of genes | 27 | |
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| Note: need to redo this experiment to confirm patterns ! |
| Good probe on gel. |
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| Expression is maintained in germ cells following gastrulation. |
| RNA localization is very dynamic, ranging from perinuclear, to apical, and finally pole cell enrichment. |
| RNA exhibits perinuclear localization syggesting association with microtubules. |
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| Good probe on gel. |
| Small foci. |
| Nice CNS. |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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| Good probe on gel. |
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| 36 ms |
| 36 ms |
| 36 ms |
| 36 ms |
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| Re-annotated. insitu done in 2006 by Eric's group. |
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| Cytoplasmic foci around the pole cells. |
| Good probe on gel (a bit low) |
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| Salivary glands and tracheal lumen, probably non-specific |
| Good probe, good plate. |
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| Re-annotated |
| Need more images to get the full story. |
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| Re-annotated |
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| Low probe on gel. Nice insitu. Interesting patterns of localization. |
| Asymmetric perinuclear localization in pole cells. |
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| Allover, enriched in pole cells. Includes apical diffuse, and small 'foci' or 'puncta'. |
| Good probe on gel. |
| Salivary glands, probably non-specific. |
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| Tracheal lumen, probably non-specific. |
| Good probe. |
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| Cytoplasmic foci around pole cells. |
| Pole plasm and pole buds enriched. |
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| Re-annotated. Original insitu done by Eric's group. |
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| Re-annotated |
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| insitu done in 2006 by Eric's group. |
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| The probe is good but low' Good insitu ------ Intersting expression. |
| Partly nascent, partly sub-nuclear. |
| Partly nascent, partly sub-nuclear. |
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| Salivary glands, probably non-specific. |
| Good probe on gel ovo transcript on X. |
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| Again, perinuclear pole cell asymmetric expression. |
| Pole cell asymmetric expression. I see small 'dots' around the pole cells, stronger in one 1/2 of the pole nuclei. |
| Good probe on gel |
| Posterior localization, even in unfertilized eggs. RNA accumulates asymmetrically around the pole buds with a 'fiber-like' texture. When nuclei divide, the RNA will stay in the nuclei that are closer to the posterior end. Very interesting! |
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| Good probe Very interesting localization! |
| Nice! |
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| Low, low probe. |