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Result Summary
| Number of genes | 28 | |
| Export | ||
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Yolk nuclei Subset yolk nuclei Random yolk nuclei Cortical nuclei Subset cortical nuclei Random cortical nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus Chromatin associated metaphase |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Cell division apparatus Chromatin associated metaphase |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Degraded Degraded partially stage 6 |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Salivary glands Posterior spiracles |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Low probe on gel, good in situ. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus Other cell division pattern Embryonic localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Yolk cortex exclusion Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei All blastoderm nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Posterior localization Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Cell division apparatus Other cell division pattern Basal localization Basal enrichment Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Yolk plasm localization Yolk plasm enrichment Degraded Degraded partially stage 7 |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Basal localization Basal enrichment Embryonic localization patterns |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic CNS |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in wing disc Wing disc pouch Wing disc few cells - dots Expressed in leg discs Leg discs particular pattern Leg discs few cells - dots Expressed in eye disc Eye disc photo-receptors Eye disc few cells - dots Eye disc apical Expressed in antennal disc Antennal disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Larval Muscles - Cytoplasmic foci Muscles allover - Larva Expressed in heart |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe Amazing pattern! |
| Neighboring nuclei, after cell division. Also, anterior to posterior gardient of the nuclear pattern (very nice). |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Random cortical nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Proventriculus Muscles-embryo Midgut Germ cell nuclei Gonads Localized |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Borderline between 'not expressed' and 'low expression' |
Adult
| Area | Images | Terms |
|---|
Comments
| Not sure if this is zygotic expression or sub-nuclear localization Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Random cortical nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
Stage 4-5 Non-Expressed |
|
| stage 6-7 |
Stage 6-7 Non-Expressed |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
Stage 10-17 Non-Expressed |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area Ventral nerve cord - nuclear localization Ventral nerve cord - subnuclear localization Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Ring gland - Few and small foci Expressed in ring gland |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel' Transcript on 2R, partly nascent, partly nuclear subregion. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Random blastoderm nuclei Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Posterior spiracles Hindgut Fat body Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in hindgut Small Foci in fat body Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands Borderline between 'not expressed' and 'low expression' |
Adult
| Area | Images | Terms |
|---|
Comments
| Possible crosscontamination, I see this early nuclear pattern in other probes from the same plate. |
| Good probe on gel. Possible crosscontamination, I see this early nuclear pattern in other probes from the same plate. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Yolk nuclei Subset yolk nuclei Random yolk nuclei Cortical nuclei All cortical nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Strong expression (1-20ms exp) Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Apical localization Apical enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Localized Subcellular localization patterns Exclusionary patterns Apical exclusion Basal localization Basal enrichment Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Inside Yolk nuclei Salivary glands Proventriculus Foregut Ectoderm Atrium Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Foci Bigger Foci Apical enrichment Apical ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. Nice data. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Random cortical nuclei Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Cytoplasmic foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Posterior spiracles Pharynx Muscles-embryo Visceral mesoderm Esophagus Dorsal vessel Blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Nuclear in pharynx Nuclear in other cells Nuclear in muscle cells nuclei Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine Borderline between 'not expressed' and 'low expression' |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Partly nascent, partly nuclear subregion. |
| Good probe on gel The transcript comes from chromosome 2L. It may be partly nascent, partly nuclear subregion. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Posterior localization RNA islands Pole plasm Pole buds Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus Other cell division pattern Embryonic localization patterns Yolk cortex localization Yolk cortex enrichment |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment Posterior localization Pole cell localization Pole cell enrichment Other subcellular pattern Cytoplasmic foci Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns Degraded Degraded partially stage 5 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Maternal Localized Posterior localization Pole cell localization Pole cell enrichment Embryonic localization patterns Degraded Degraded partially stage 6 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in amnioserosa Degraded Degraded completely stage 8 Degradation maternal transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Dorsal trunk foci Salivary glands Proventriculus Muscles-embryo Muscles - subregion Midgut Midgut subset Ectoderm CNS Ventral nerve cord Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around midgut nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in muscle cells Small Foci in midgut Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| RNA exhibits cortical enrichment and posterior pole plasm localization. |
| RNA exhibits pole cell enrichment and is enriched apically in the posterior blastoderm nuclei. |
| Salivary glands, my be non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Random cortical nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
|
Stage 4-5 Expressed Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Membrane associated Degraded Degraded partially stage 7 |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Localized Subcellular localization patterns Membrane associated Foci Small Foci |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. Data from probe 'RE04126'. |
| Borderline between 'not expressed' and 'low expression' |
| Salivary glands and tracheal lumen, probably non-specific. |
| small foci. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Other subcellular pattern Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
|
Stage 4-5 Expressed Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Apical localization Apical enrichment Apical clusters Embryonic localization patterns |
| stage 6-7 |
Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 |
|
| stage 8-9 |
Stage 8-9 Non-Expressed |
|
| stage 10-17 |
Stage 10-17 Non-Expressed |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Not expressed in the intestine |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Not expressed in nervous system |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Expressed in lymph glands Lymph glands all over |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel Amazing localization, 'nuclear donuts'. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Yolk nuclei Subset yolk nuclei Random yolk nuclei Cortical nuclei Subset cortical nuclei Random cortical nuclei Anterior cortical nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 7 |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Ubiquitous |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Proventriculus allover Expressed in midgut Subcellular localization in midgut Midgut - foci Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in gastric caeca Gastric caeca allover Expressed in esophagus |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in eye disc Eye disc few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Expressed in brain lobes Brain lobes - Foci or dots |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. Not sure if the nuclear/ zygotic expression/ localization is real... |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns Degraded Degraded partially stage 4 |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Proventriculus Posterior spiracles Midgut Hindgut Specific hindgut area or cells Germ cell nuclei Gonads |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in gastric caeca Gastric caeca allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel Not sure if the sub-nuclear localization is real (I see other probes like this in the same plate...?). Maybe cross-contamination. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei Subset cortical nuclei Random cortical nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Non-Expressed Degraded Degraded completely stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Head Fat body Brain Atrium Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Foci. Nothing during mitosis at the level of the chromatin... the foci are found below - closer to the yolk. |
| Good probe on gel. Partly nascent, partly nuclear subregion |
| Allover, enriched in some tissues. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Zygotic starting stage 6 Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 6 |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous Localized Subcellular localization patterns Cytoplasmic foci |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Yolk nuclei 'foci' Tracheal system Muscles-embryo Muscles - subregion Muscles - 'podia' Localized Subcellular localization patterns Nuclei-associated localization Nuclear in muscle cells nuclei Foci Small Foci Small Foci in tracheal cells Small Foci in muscle cells Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Not expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Subcellular localization in proventriculus Proventriculus foci Particular proventriculus area |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - nuclear dots Salivary gland - Cytoplasmic foci Nascent transcript in salivary gland |
| ovary and testis |
|
Adult Tissues Male reproductive tissues Testis 3rd Instar Larval Tissues Larval Ovary and Testis-Larva Specific testis expression localization Larval spermatocytes |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Expressed in lymph glands |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatogonium-TE Cytoplasmic in spermatogonium-TE Spermatocytes -TE Late Spermatocytes- TE Cytoplasmic in late spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Cytoplasmic in round spermatids-TE Cytoplasmic foci in round spermatids - TE Cyst cells -TE Tail cyst cell - TE Individualizing Tail Cyst Cell - TE Individualizing Tail Cyst Cell Cytoplasm - TE Elongating Tail Cyst Cell - TE Elongating Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Early cyst cell Cytoplasmic in early cyst cell Cytoplasmic foci in early cyst cell - TE Lower expression in tip region Cytoplasmic Seminal Vesicle Expression level-SV Expressed Medium - SV Cell type - SV Cytoplasmic-SV Membrane enriched - SV Cytoplasmic Foci - SV Basal Enrichment-SV Ejaculatory Duct Subcellularly localized - ED Lumenal-ED Sleeve - ED Non-particulate - ED Outer lumen - ED Anterior ejaculatory duct papilla-ED Cytoplasmic in anterior ejaculatory papilla-ED Expression level-ED Expressed Medium - ED Cell type - ED Cytoplasmic-ED Cytoplasmic Foci - ED Diffuse localization-ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG ECM like pattern-AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Cytoplasmic-SC Multivesicular bodies - SC Main cells -AG Cytoplasmic-MC |
Comments
Probe GM08252 Links: Permalink
Genes: flam flam-intron
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 1 Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Pronucleus-associated Perinuclear localization Perinuclear around all nuclei Intranuclear accumulation Accumulation in all nuclei Cell division apparatus Chromatin associated metaphase Local terms for zygotic transcripts |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei All yolk nuclei Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Pole cell localization Pole cell enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci Cell division apparatus Chromatin associated metaphase Local terms for zygotic transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei All blastoderm nuclei Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus Chromatin associated metaphase Local terms for zygotic transcripts |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei All blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear around yolk nuclei Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Apical localization Local terms for zygotic transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Tracheal system Specific tracheal branches Segmented pattern Salivary glands Posterior spiracles Specific posterior spiracle pattern Midgut Head Head epidermis Ectoderm Atrium Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion Foci Small Foci Small Foci in tracheal cells Small Foci in other tissues Small Foci in ectoderm |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Fat body - Nuclear localization Fat body - Subnuclear localization Nascent transcript in fat body Expressed in fat body |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in proventriculus Proventriculus allover Particular proventriculus area Expressed in midgut Subcellular localization in midgut Midgut - foci Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - Nuclear in Malpighian tubules - Subnuclear in Nascent transcript in malpighian tubules Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Nuclear in hindgut Hindgut - subnuclear localization Hindgut foci Nascent transcript in hindgut Hindgut allover Expressed in gastric caeca Subcellular localization in gastric caeca Gastric caeca - nuclear localization Gastric caeca - subnuclear localization Nascent transcript in gastric caeca Gastric caeca allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs particular pattern Leg discs few cells - dots Expressed in eye disc Morphogenetic furrow Eye disc photo-receptors Eye disc few cells - dots Eye disc - center round cells Expressed in antennal disc |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area Ventral nerve cord - foci or 'dots' Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Subcellular localization in salivary gland Salivary gland - subnuclear Salivary gland - nuclear dots Salivary gland - Cytoplasmic foci Cytoplasmic foci in Salivary Gland Lumen Salivary gland - apical Salivary gland - lumen Nascent transcript in salivary gland Expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Nuclear Ring gland - Subnuclear Nascent transcript in ring gland Expressed in ring gland |
Adult
| Area | Images | Terms |
|---|---|---|
| Male reproductive tissues |
|
Adult Tissues Male reproductive tissues Testis Testis Cell type and stage - TE Spermatocytes -TE Y-Loop pattern - TE Nuclear in Spermatocytes - TE Cytoplasmic in Spermatocytes - TE Spermatids -TE Round spermatids-TE Nuclear in round spermatids-TE Cytoplasmic in round spermatids-TE Elongating Spermatids - TE Cytoplasmic in elongating spermatids-TE Cyst cells -TE Tail cyst cell - TE Sperm Tail Decoration-TCC associated Retracting/Coiling sperm tail-TCC associated Retracting/Coiling Tail Cyst Cell - TE Retracting/Coiling Tail Cyst Cell Cytoplasm - TE Subcellularly localized - TE Nuclear Expression level-TE Expressed Medium - TE Distal tip region-TE Expressed in distal tip region - TE Specific tip region cells-TE Early cyst cell Cytoplasmic in early cyst cell Lower expression in tip region Cytoplasmic Seminal Vesicle Subcellularly localized - SV Lumenal-SV Scattered Foci - SV ECM like pattern-SV Expression level-SV Expressed Medium - SV Cell type - SV Sperm Tail Decor - SV Cytoplasmic-SV Cytoplasmic Foci - SV Ejaculatory Duct Subcellularly localized - ED Expression level-ED Expressed Low - ED Cell type - ED Nuclear-ED Nuclear associated - Ejaculatory Duct Subnuclear - Ejaculatory Duct Nuclear Foci - Ejaculatory Duct Cytoplasmic-ED Membrane enriched - ED Cytoplasmic Foci - ED Accessory Gland Subcellularly localized - AG Lumenal - AG Non-particulate - AG Expression level-AG Expressed Medium - AG Cytoplasmic Cell type - AG Secondary cells - AG Nuclear-SC Nuclear Foci-SC Cytoplasmic-SC Multivesicular bodies - SC Main cells -AG Nuclear- MC Nuclear foci-mc Cytoplasmic-MC Cytoplasmic Foci - AG |
Comments
| The probe may be recognizing 16 transcripts - Including flamenco. The probe may recognize some other areas in the genome that contain a highly homologous sequence. |
| Amazing patterns! |
| Very interesting patterns in adult testes and associated tissues. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Basal localization |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Localized Yolk plasm localization Yolk plasm enrichment Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion Basal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Yolk nuclei All yolk nuclei Germ cell nuclei All pole cell nuclei Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion Basal localization Germ cell localization Germ cell enrichment |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Foregut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Basal enrichment |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Perinuclear localization Perinuclear around all nuclei Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Ubiquitous Medium expression (21-40ms exp) Maternal Localized Subcellular localization patterns Pole cell localization Nuclei-associated localization Perinuclear localization Perinuclear around all nuclei Exclusionary patterns Apical exclusion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei All pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Ubiquitous Medium expression (21-40ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Apical exclusion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei All pole cell nuclei Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around all nuclei |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Midgut Hindgut Foregut Ubiquitous Strong expression (1-20ms exp) Localized Subcellular localization patterns Perinuclear localization Perinuclear around midgut nuclei Perinuclear around hindgut nuclei Foci Small Foci Small Foci in midgut Small Foci in hindgut |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Maternal Degraded Degraded partially stage 7 Degradation maternal transcripts |
| stage 8-9 |
|
Stage 8-9 Non-Expressed |
| stage 10-17 |
|
Stage 10-17 Non-Expressed |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs Leg discs particular pattern Leg discs few cells - dots |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes - Foci or dots |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Not 100% pole cell excluded. |
| Good probe in gel. Nuclear subregion. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Embryonic localization patterns |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Embryonic localization patterns Degraded Degraded partially stage 4 Degradation maternal transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Exclusionary patterns Embryonic localization patterns Exclusionary patterns Pole cell exclusion |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Salivary glands Salivary glands lumen Posterior spiracles Hindgut Localized |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. Potential crosscontamination, I see the same intra-nuclear pattern in other probes of the same plate. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Yolk nuclei Cortical nuclei Ubiquitous Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Yolk nuclei All yolk nuclei Blastoderm nuclei All blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei All yolk nuclei Blastoderm nuclei All blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei All blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Blastoderm nuclei Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -also known as scaRNA:MeU5-C46 |
| -tested using HCR-FISH using a 5 probe sets |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Cortical nuclei Subset cortical nuclei Random cortical nuclei Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
|
Stage 4-5 Expressed Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 6 |
| stage 8-9 |
|
Stage 8-9 Non-Expressed Degraded Degraded completely stage 9 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Posterior spiracles Muscles-embryo Midgut Hindgut Specific hindgut area or cells Foregut Fat body Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Foci Small Foci Small Foci in muscle cells Small Foci in midgut Small Foci in hindgut Small Foci in foregut |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in midgut Subcellular localization in midgut Midgut - foci Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in gastric caeca Gastric caeca allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - foci or 'dots' Localized to axons Expressed in brain lobes Brain lobes allover |
| salivary gland |
|
3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Ring gland - Subcellular localization Ring gland - Cytoplasmic foci Expressed in ring gland Ring gland allover |
Adult
| Area | Images | Terms |
|---|
Comments
| Tracheal lumen probably non-specific. |
| Good probe on gel. Not sure if the sub-nuclear localization is real, I see a similar pattern in other wells of the same 96 well plate (?) |
| It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus Other cell division pattern Embryonic localization patterns Exclusionary patterns Pole plasm excluded |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Zygotic starting stage 4 Yolk nuclei Subset yolk nuclei Random yolk nuclei Blastoderm nuclei All blastoderm nuclei Transiently widespread Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Exclusionary patterns Pole cell exclusion Cell division apparatus Spindle midzone localization Chromatin associated metaphase Apical localization Apical enrichment |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Yolk Nuclei Subset yolk nuclei Random yolk nuclei Weak expression (>41ms exp) Ubiquitous Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous Degraded Degraded partially stage 8 Degradation maternal transcripts |
| stage 10-17 |
|
Stage 10-17 Expressed Weak expression (>41ms exp) Ubiquitous |
Larva
| Area | Images | Terms |
|---|---|---|
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Nascent transcript in hindgut Hindgut allover |
| imaginal discs |
|
3rd Instar Larval Tissues Imaginal discs Expressed in leg discs |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in ventral nerve cord Ventral nerve cord - particular area Expressed in brain lobes Optic lobe Brain lobes - Significant area |
| lymph glands and ring glands |
|
3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Expressed in lymph glands Lymph glands all over |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. Nice in situ. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Ubiquitous Strong expression (1-20ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 4-5 |
|
Stage 4-5 Expressed Ubiquitous Strong expression (1-20ms exp) |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous |
| stage 8-9 |
|
Stage 8-9 Expressed Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic CNS Ventral nerve cord Polarized VNC Brain Ubiquitous |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting common exons |
| -BDGP does not observe staining, however, modENCODE RNAseq data on FlyBase shows an expression profile that more closely aligns with our observations |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Yolk nuclei Subset yolk nuclei Random yolk nuclei Ubiquitous Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Pole cell exclusion Apical exclusion Cytoplasmic foci Basal localization Basal enrichment Degraded Degraded partially stage 4 Degradation zygotic transcripts |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Posterior blastoderm nuclei Expression in ectoderm Expression in API Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci Basal localization Basal enrichment Posterior localization Embryonic localization patterns Ventral localization Dorsal localization Anterior localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Ventral blastoderm nuclei Segmented pattern Segment polarity pattern Expression in neuroblasts Expression in ectoderm Expression in API Localized Subcellular localization patterns Perinuclear localization Perinuclear around blastoderm nuclei Cytoplasmic foci Basal localization Basal enrichment Embryonic localization patterns Anterior localization |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Sensory system Segmented pattern PNS Optic lobes primordium Neuroblasts Polarized Neuroblasts Midgut Maxillary complex Head Head epidermis CNS Ventral nerve cord Polarized VNC Perinuclear VNC Foci - VNC |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -expression seen in the stomatogastric sensory primordium, and the anterior and posterior midgut primordium; forms a complex along with ac (CG3796) so should colocalize in neuronal regions |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Yolk nuclei Subset yolk nuclei Random yolk nuclei Cortical nuclei All cortical nuclei Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cell division apparatus Other cell division pattern |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Segment polarity pattern Gap-gene pattern Weak expression (>41ms exp) Localized Subcellular localization patterns Nuclei-associated localization Intranuclear accumulation Accumulation nuclear subregion Accumulation in subset nuclei Accumulation entire nucleus Exclusionary patterns Pole cell exclusion Basal exclusion Apical localization Apical enrichment Diffuse apical localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Segmented pattern Terminal-gene pattern |
| stage 8-9 |
|
Stage 8-9 Non-Expressed Degraded Degraded completely stage 8 |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Yolk nuclei Inside Yolk nuclei Localized Subcellular localization patterns Nuclei-associated localization Accumulation nuclear subregion |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. Partly nascent, partly nuclear subregion |
| Salivary glands and tracheal lumen, probably non-specific. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion Cytoplasmic foci Cell division apparatus Centrosomal localization |
| stage 4-5 |
|
Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Pole cell localization Cytoplasmic foci Cell division apparatus Centrosomal localization Apical localization Apical enrichment Discrete apical foci Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Ubiquitous Maternal |
| stage 8-9 |
|
Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 8 Degradation maternal transcripts |
| stage 10-17 |
Stage 10-17 Expressed |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| Small foci. |
| Re-annotated. Very interesting in situ patterns. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Cortical nuclei All cortical nuclei Weak expression (>41ms exp) Ubiquitous Maternal Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation nuclear subregion |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Blastoderm nuclei Subset blastoderm nuclei Expression in mesoderm Localized Subcellular localization patterns Basal localization Basal enrichment |
| stage 8-9 |
|
Stage 8-9 Expressed Weak expression (>41ms exp) Ubiquitous |
| stage 10-17 |
|
Stage 10-17 Expressed Zygotic Salivary glands CNS Ventral nerve cord Axons Localized Subcellular localization patterns Membrane associated Cell extensions or 'podia' |
Larva
| Area | Images | Terms |
|---|---|---|
| fat body |
|
3rd Instar Larval Tissues Fat body Expressed in fat body Fat body allover - Ubiquitous |
| intestine |
|
3rd Instar Larval Tissues Intestine Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules allover Expressed in hindgut Subcellular localization in hindgut Hindgut foci Few hindgut foci Hindgut allover |
| nervous system |
|
3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes allover Brain lobes - Foci or dots |
| muscles |
|
3rd Instar Larval Tissues Larval Muscles - larva Expressed in larval muscles-Larva Sub-cellular localization in muscles-Larva Localized to larval muscle band Larval Muscles - Cytoplasmic foci Muscles allover - Larva |
Adult
| Area | Images | Terms |
|---|
Comments
| Good probe on gel. |
Embryo
| Area | Images | Terms |
|---|---|---|
| stage 1-3 |
|
Stage 1-3 Expressed Zygotic Zygotic starting stage 3 Ubiquitous Localized Subcellular localization patterns Nuclei associated localization Intranuclear accumulation Accumulation in subset nuclei Cytoplasmic foci |
| stage 4-5 |
|
Stage 4-5 Expressed Zygotic Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Posterior blastoderm nuclei Dorsal blastoderm nuclei Anterior blastoderm nuclei Localized Subcellular localization patterns Yolk cortex localization Yolk cortex enrichment Ventral localization Posterior localization Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around blastoderm nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Basal localization Apical localization Apical enrichment Anterior localization Embryonic localization patterns |
| stage 6-7 |
|
Stage 6-7 Expressed Zygotic Pole cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Expression in API Dorsal blastoderm nuclei Localized Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around pole cell nuclei Perinuclear around blastoderm nuclei Intranuclear accumulation Accumulation in subset nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Cytoplasmic foci Pole cell localization Pole cell enrichment Embryonic localization patterns Dorsal localization |
| stage 8-9 |
|
Stage 8-9 Expressed Zygotic Germ cell nuclei Subset pole cell nuclei Blastoderm nuclei Subset blastoderm nuclei Expression in ectoderm Expression in API Expression in amnioserosa Dorsal blastoderm nuclei Localized Yolk cortex localization Yolk cortex enrichment Subcellular localization patterns Perinuclear localization Perinuclear around pole cell nuclei Exclusionary patterns Yolk plasm exclusion Yolk cortex exclusion Cytoplasmic foci Basal localization Germ cell localization Germ cell enrichment Embryonic localization patterns Dorsal localization Degraded Degraded partially stage 9 |
| stage 10-17 |
Stage 10-17 Non-Expressed |
Larva
| Area | Images | Terms |
|---|
Adult
| Area | Images | Terms |
|---|
Comments
| -performed via HCR-FISH using 3 probe sets targeting the RA transcript isoform |
| -neat expression patterns in early embryos and early germ band elongation stages; expression is no longer detectable after stage 9-10 (fits modENCODE RNA-seq data well) |