twi CG2956

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Ubiquitous   Medium expression (21-40ms exp)   Maternal  Degraded   Degraded partially stage 2   Degradation maternal transcripts
stage 4-5		Stage 4-5  Expressed   Zygotic    Zygotic starting stage 4    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei   Strong expression (1-20ms exp)   Localized    Subcellular localization patterns     Nuclei-associated localization      Perinuclear localization       Perinuclear subset of nuclei        Perinuclear around blastoderm nuclei      Intranuclear accumulation       Accumulation in subset nuclei     Membrane associated     Exclusionary patterns      Pole cell exclusion     Cytoplasmic foci     Apical localization      Apical enrichment       Diffuse apical localization    Local terms for zygotic transcripts    Embryonic localization patterns  Degraded   Degraded partially stage 4   Degradation maternal transcripts
stage 6-7		Stage 6-7  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Expression in mesoderm   Strong expression (1-20ms exp)
stage 8-9		Stage 8-9  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Expression in mesoderm   Strong expression (1-20ms exp)
stage 10-17		Stage 10-17  Expressed   Zygotic    Tracheal system     Tracheal pits    Segmented pattern    Ectoderm   Localized

Area	Images	Terms

Area	Images	Terms
Male reproductive tissues

Need more images to get the full story. In situ done before we started screening late stage embryos.

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Ubiquitous   Medium expression (21-40ms exp)   Maternal
stage 4-5		Stage 4-5  Expressed   Zygotic    Zygotic starting stage 4    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei   Medium expression (21-40ms exp)   Localized    Subcellular localization patterns     Exclusionary patterns      Pole cell exclusion     Apical localization      Apical enrichment       Diffuse apical localization    Embryonic localization patterns  Degraded   Degraded partially stage 4   Degradation maternal transcripts
stage 6-7		Stage 6-7  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei      Expression in mesoderm   Medium expression (21-40ms exp)
stage 8-9		Stage 8-9  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Expression in mesoderm   Medium expression (21-40ms exp)
stage 10-17		Stage 10-17  Expressed   Zygotic

Area	Images	Terms
fat body		3rd Instar Larval Tissues  Fat body   Not expressed in fat body
intestine		3rd Instar Larval Tissues  Intestine   Not expressed in the intestine
imaginal discs		3rd Instar Larval Tissues  Imaginal discs   Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues  Nervous system   Not expressed in nervous system
salivary gland		3rd Instar Larval Tissues  Salivary gland   Not expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues  Lymph Glands and Ring Glands   Ring gland    Not expressed in ring gland

Area	Images	Terms

Area	Images	Terms
stage 1-3		Stage 1-3  Non-Expressed
stage 4-5		Stage 4-5  Expressed   Zygotic    Zygotic starting stage 4    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei      Posterior blastoderm nuclei      Anterior blastoderm nuclei   Localized    Subcellular localization patterns     Ventral localization     Posterior localization     Nuclei-associated localization      Perinuclear localization       Perinuclear subset of nuclei        Perinuclear around blastoderm nuclei     Exclusionary patterns      Yolk plasm exclusion      Pole cell exclusion     Cytoplasmic foci     Basal localization      Basal enrichment     Apical localization      Apical enrichment       Diffuse apical localization     Anterior localization
stage 6-7		Stage 6-7  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei      Expression in mesoderm      Expression in ectoderm      Anterior blastoderm nuclei   Localized    Yolk cortex localization     Yolk cortex enrichment    Subcellular localization patterns     Nuclei-associated localization      Perinuclear localization       Perinuclear subset of nuclei        Perinuclear around blastoderm nuclei     Exclusionary patterns      Yolk cortex exclusion     Basal localization      Basal enrichment     Apical localization      Apical enrichment       Diffuse apical localization    Posterior localization    Embryonic localization patterns     Anterior localization
stage 8-9		Stage 8-9  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Segmented pattern       Pair-rule pattern      Expression in mesoderm      Expression in ectoderm      Expression in API   Localized    Subcellular localization patterns     Perinuclear localization      Perinuclear around blastoderm nuclei     Cytoplasmic foci     Basal localization      Basal enrichment    Embryonic localization patterns     Ventral localization     Germ band localization     Dorsal localization     Anterior localization  Degraded   Degraded partially stage 9
stage 10-17		Stage 10-17  Expressed   Zygotic    Muscles-embryo     Visceral mesoderm      Specific visceral mesoderm pattern     Muscles - subregion    Hindgut    Head    Foregut    Esophagus    Ectoderm   Localized    Subcellular localization patterns     Perinuclear localization      Perinuclear around muscle nuclei

Area	Images	Terms

Area	Images	Terms

-retested using HCR-FISH 2025 for full coverage of late stage expression patterns

-performed using 3 probe pairs (HCR-FISH) against the RB isoform

-really interesting patterns!