shd CG13478

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Weak expression (>41ms exp)   Maternal   Localized    Embryonic localization patterns     Exclusionary patterns      Yolk cortex exclusion      Pole plasm excluded
stage 4-5		Stage 4-5  Expressed   Weak expression (>41ms exp)   Maternal   Localized    Subcellular localization patterns     Yolk plasm localization      Yolk plasm enrichment     Exclusionary patterns      Pole cell exclusion      Basal exclusion      Apical exclusion    Embryonic localization patterns

Area	Images	Terms

Area	Images	Terms

Re-annotated. Need more data.

Area	Images	Terms
stage 1-3		Stage 1-3  Non-Expressed
stage 4-5		Stage 4-5  Non-Expressed
stage 6-7		Stage 6-7  Non-Expressed
stage 8-9		Stage 8-9  Expressed   Zygotic    Zygotic starting stage 8    Blastoderm nuclei     Subset blastoderm nuclei      Segmented pattern       Pair-rule pattern      Expression in ectoderm   Localized    Subcellular localization patterns     Nuclei-associated localization      Accumulation in subset nuclei     Cytoplasmic foci    Embryonic localization patterns     Ventral localization     Dorsal localization
stage 10-17		Stage 10-17  Expressed   Zygotic    Tracheal system     Tracheal pits    Segmented pattern    Posterior spiracles    Head     Head epidermis    Ectoderm     Polarized Ectoderm   Ubiquitous   Localized    Subcellular localization patterns     Nuclei-associated localization      Nuclear in ectoderm     Foci      Small Foci       Small Foci in ectoderm      Bigger Foci     Apical enrichment      Apical ectoderm

Area	Images	Terms

Area	Images	Terms

-performed via HCR-FISH using 6 probe sets targeting the RD isoform transcript (previously tested with 3 and was not detected)

-probes are from two different plates (3 sets from each plate); SH-HCR-Plate-1 and SH-HCR-Plate-4

-this gene is very weakly expressed and more probe sets (if using HCR-FISH) should be considered for future retests