GEFmeso CG30115

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Ubiquitous   Medium expression (21-40ms exp)   Maternal
stage 4-5		Stage 4-5  Expressed   Zygotic    Zygotic starting stage 4    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei   Medium expression (21-40ms exp)   Maternal   Localized    Subcellular localization patterns     Exclusionary patterns      Pole cell exclusion      Apical exclusion     Apical localization      Apical enrichment    Embryonic localization patterns  Degraded   Degraded partially stage 4   Degradation maternal transcripts
stage 6-7		Stage 6-7  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei      Segmented pattern      Expression in ectoderm   Medium expression (21-40ms exp)
stage 8-9		Stage 8-9  Expressed   Zygotic   Medium expression (21-40ms exp)
stage 10-17		Stage 10-17  Expressed   Zygotic    Tracheal system     Tracheal pits    Segmented pattern    Ectoderm

Area	Images	Terms

Area	Images	Terms

-missing late stage coverage; candidate for retesting (2025)

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Weak expression (>41ms exp)   Ubiquitous   Maternal
stage 4-5		Stage 4-5  Expressed   Zygotic    Zygotic starting stage 4    Yolk nuclei     Subset yolk nuclei      Random yolk nuclei    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei
stage 6-7		Stage 6-7  Expressed   Zygotic    Yolk Nuclei     Subset yolk nuclei      Random yolk nuclei    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei      Random blastoderm nuclei
stage 8-9		Stage 8-9  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Segmented pattern      Expression in mesoderm

Area	Images	Terms

Area	Images	Terms

Re-annotated

-missing late stage coverage; candidate for retesting (2025)

Area	Images	Terms
stage 1-3		Stage 1-3  Non-Expressed
stage 4-5		Stage 4-5  Expressed   Zygotic    Zygotic starting stage 4    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei      Posterior blastoderm nuclei   Localized    Subcellular localization patterns     Nuclei-associated localization      Perinuclear localization       Perinuclear subset of nuclei
stage 6-7		Stage 6-7  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Segmented pattern       Pair-rule pattern      Expression in mesoderm   Localized    Subcellular localization patterns     Cytoplasmic foci    Embryonic localization patterns     Ventral localization
stage 8-9		Stage 8-9  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Segmented pattern       Segment polarity pattern       Pair-rule pattern      Expression in mesoderm   Localized    Embryonic localization patterns     Ventral localization     Posterior localization     Germ band localization
stage 10-17		Stage 10-17  Expressed   Zygotic    Segmented pattern    PNS    Muscles-embryo     Visceral mesoderm    Fat body     Fat body- specific region    Ectoderm    Dorsal vessel   Localized    Subcellular localization patterns     Foci      Small Foci       Small Foci in ectoderm

Area	Images	Terms

Area	Images	Terms

-performed via HCR-FISH using 3 probe sets targeting common exons