CG6885

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Zygotic    Zygotic starting stage 3    Cortical nuclei     Subset cortical nuclei      Random cortical nuclei   Weak expression (>41ms exp)   Localized    Subcellular localization patterns     Cytoplasmic foci
stage 4-5		Stage 4-5  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Random blastoderm nuclei   Weak expression (>41ms exp)   Localized    Subcellular localization patterns     Nuclei-associated localization      Intranuclear accumulation       Accumulation nuclear subregion
stage 6-7		Stage 6-7  Expressed   Weak expression (>41ms exp)   Ubiquitous
stage 8-9		Stage 8-9  Expressed   Zygotic    Zygotic starting stage 9    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei   Weak expression (>41ms exp)   Ubiquitous  Degraded   Degraded partially stage 9   Degradation maternal transcripts
stage 10-17		Stage 10-17  Expressed   Zygotic    CNS     Ventral nerve cord      Foci - VNC    Brain    Blastoderm nuclei   Localized    Subcellular localization patterns     Foci      Small Foci       Small Foci in CNS       Small Foci in Brain

Area	Images	Terms
fat body		3rd Instar Larval Tissues  Fat body   Not expressed in fat body
intestine		3rd Instar Larval Tissues  Intestine   Not expressed in the intestine
imaginal discs		3rd Instar Larval Tissues  Imaginal discs   Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues  Nervous system   Not expressed in nervous system
salivary gland		3rd Instar Larval Tissues  Salivary gland   Not expressed in salivary glands
lymph glands and ring glands		3rd Instar Larval Tissues  Lymph Glands and Ring Glands   Lymph Glands    Not expressed in lymph glands

Area	Images	Terms
Male reproductive tissues		Adult Tissues  Male reproductive tissues   Testis    Testis Cell type and stage - TE     Spermatogonium-TE      Cytoplasmic in spermatogonium-TE       Cytoplasmic foci in spermatogonium - TE     Spermatocytes -TE      Late Spermatocytes- TE       Cytoplasmic in late spermatocytes - TE      Cytoplasmic in Spermatocytes - TE     Spermatids -TE      Round spermatids-TE       Cytoplasmic in round spermatids-TE      Elongating Spermatids - TE       Cytoplasmic in elongating spermatids-TE      Early elongating spermatids-TE       Cytoplasmic in early elongating spermatids     Muscle cells - TE      Cytoplasmic in muscle cells - TE     Cyst cells -TE      Tail cyst cell - TE       Spermatocyte Tail Cyst Cell - TE        Spermatocyte Tail Cyst Cell Cytoplasm - TE       Elongating Tail Cyst Cell - TE        Elongating Tail Cyst Cell Cytoplasm - TE    Subcellularly localized - TE    Expression level-TE     Expressed Low - TE    Distal tip region-TE     Expressed in distal tip region - TE      Lower expression in tip region   Seminal Vesicle    Subcellularly localized - SV    Lumenal-SV     Non-particulate - SV    Expression level-SV     Expressed Low - SV    Cell type - SV     Cytoplasmic-SV      Membrane enriched - SV      Cytoplasmic Foci - SV   Ejaculatory Duct    Subcellularly localized - ED    Lumenal-ED     Anterior ejaculatory duct papilla-ED      Entry ring-ED       Cytoplasmic in entry ring-ED      Cytoplasmic in anterior ejaculatory papilla-ED    Expression level-ED     Expressed Low - ED    Cell type - ED     Cytoplasmic-ED      Membrane enriched - ED      Cytoplasmic Foci - ED   Accessory Gland    Subcellularly localized - AG    Lumenal - AG     Non-particulate - AG    Expression level-AG     Expressed Low - AG    Cell type - AG     Secondary cells - AG      Cytoplasmic-SC     Main cells -AG      Cytoplasmic-MC       Clusters - AG    Basal Enrichment - MC

Good probe on gel.

Salivary glands, probably non-specific.

Nuclear subregion.

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Zygotic    Zygotic starting stage 3    Cortical nuclei     All cortical nuclei   Ubiquitous   Localized    Subcellular localization patterns     Nuclei associated localization     Cytoplasmic foci
stage 4-5		Stage 4-5  Expressed   Zygotic    Yolk nuclei     Subset yolk nuclei      Random yolk nuclei    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei      Dorsal blastoderm nuclei     All blastoderm nuclei      Transiently widespread   Ubiquitous   Localized    Subcellular localization patterns     Yolk plasm localization     Ventral localization     Nuclei-associated localization      Perinuclear localization       Perinuclear subset of nuclei        Perinuclear around yolk nuclei        Perinuclear around blastoderm nuclei      Intranuclear accumulation       Accumulation in subset nuclei     Exclusionary patterns      Pole cell exclusion     Dorsal localization     Cytoplasmic foci
stage 6-7		Stage 6-7  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Expression in mesoderm      Expression in ectoderm      Expression in API     All blastoderm nuclei   Ubiquitous   Localized    Subcellular localization patterns     Nuclei-associated localization     Cytoplasmic foci
stage 8-9		Stage 8-9  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Expression in mesoderm      Expression in ectoderm      Expression in API     All blastoderm nuclei      Transiently widespread   Ubiquitous   Localized    Subcellular localization patterns     Cytoplasmic foci    Germ cell localization     Germ cell enrichment  Degraded   Degraded partially stage 9
stage 10-17		Stage 10-17  Non-Expressed

Area	Images	Terms

Area	Images	Terms

-probes were designed against RA isoform

-tested using HCR-FISH probes (3 probe sets)

-expression pattern aligns well with modENCODE RNA-seq data -observed possible early lateral or dorsal-ventral localization of early ectoderm

-bright foci is mostly background