Mef2 CG1429

Area	Images	Terms

Area	Images	Terms

Area	Images	Terms

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Weak expression (>41ms exp)   Ubiquitous   Maternal
stage 4-5		Stage 4-5  Expressed   Zygotic    Zygotic starting stage 5    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei  Degraded   Degraded completely stage 4   Degradation maternal transcripts
stage 6-7		Stage 6-7  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei      Expression in mesoderm
stage 8-9		Stage 8-9  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Expression in mesoderm
stage 10-17		Stage 10-17  Expressed   Zygotic    CNS     Ventral nerve cord    Blastoderm nuclei   Localized

Area	Images	Terms

Area	Images	Terms

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Weak expression (>41ms exp)   Ubiquitous   Maternal
stage 4-5		Stage 4-5  Expressed   Weak expression (>41ms exp)   Ubiquitous   Maternal
stage 6-7		Stage 6-7  Expressed   Weak expression (>41ms exp)   Ubiquitous   Maternal
stage 8-9		Stage 8-9  Expressed   Ubiquitous
stage 10-17		Stage 10-17  Expressed   Zygotic    Hemocytes     Hemocytes specific area    Blastoderm nuclei   Localized    Subcellular localization patterns     Perinuclear localization      Perinuclear around hemocytes     Nuclei-associated localization      Nuclear in hemocytes

Area	Images	Terms

Area	Images	Terms

Salivary glands, probably non-specific.

Area	Images	Terms
stage 1-3		Stage 1-3  Non-Expressed
stage 4-5		Stage 4-5  Non-Expressed
stage 6-7		Stage 6-7  Expressed   Zygotic    Zygotic starting stage 6    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei      Expression in mesoderm   Weak expression (>41ms exp)   Localized    Subcellular localization patterns     Nuclei-associated localization      Intranuclear accumulation       Accumulation in subset nuclei    Embryonic localization patterns     Ventral localization
stage 8-9		Stage 8-9  Expressed   Zygotic    Blastoderm nuclei     Subset blastoderm nuclei      Expression in mesoderm   Localized    Subcellular localization patterns     Nuclei-associated localization      Accumulation in subset nuclei     Cytoplasmic foci    Embryonic localization patterns     Germ band localization
stage 10-17		Stage 10-17  Expressed   Zygotic    Muscles-embryo     Visceral mesoderm    Head   Localized    Subcellular localization patterns     Nuclei-associated localization      Nuclear in muscle cells nuclei     Foci      Small Foci       Small Foci in muscle cells       Small Foci in head

Area	Images	Terms

Area	Images	Terms

-performed via HCR-FISH using 3 probe sets targeting the RI isoform transcript; difficult to pick best isoform to design probes to due to vast number of them, hard to say if these probes captured the complete expression profile for this gene

-Our previous experiments using TSA and independent experiments by BDGP observed maternal expression in stages 1-3 and stages 4-5, this was not observed in this experiment

-Interesting early mesoderm marker