CR45138

Area	Images	Terms
stage 1-3		Stage 1-3  Expressed   Weak expression (>41ms exp)   Ubiquitous   Maternal   Localized    Subcellular localization patterns     Nuclei associated localization      Perinuclear localization       Perinuclear around all nuclei     Cytoplasmic foci
stage 4-5		Stage 4-5  Expressed   Zygotic   Ubiquitous   Medium expression (21-40ms exp)   Maternal   Localized    Subcellular localization patterns     Nuclei-associated localization      Perinuclear localization       Perinuclear around all nuclei     Exclusionary patterns      Apical exclusion
stage 6-7		Stage 6-7  Expressed   Zygotic    Pole cell nuclei     All pole cell nuclei   Ubiquitous   Medium expression (21-40ms exp)   Localized    Subcellular localization patterns     Nuclei-associated localization      Perinuclear localization       Perinuclear subset of nuclei        Perinuclear around yolk nuclei        Perinuclear around blastoderm nuclei
stage 8-9		Stage 8-9  Expressed   Zygotic    Yolk nuclei     All yolk nuclei    Germ cell nuclei     All pole cell nuclei    Blastoderm nuclei     All blastoderm nuclei      Persistent widespread   Ubiquitous   Strong expression (1-20ms exp)   Medium expression (21-40ms exp)
stage 10-17		Stage 10-17  Expressed   Zygotic    Midgut    Hindgut   Ubiquitous   Strong expression (1-20ms exp)   Medium expression (21-40ms exp)   Localized    Subcellular localization patterns     Nuclei-associated localization      Nuclear in midgut nuclei      Nuclear in hindgut nuclei     Foci      Small Foci       Small Foci in midgut       Small Foci in foregut      Bigger Foci

Area	Images	Terms

Area	Images	Terms

-sample background seems to high and does not match current data - candidate for retest (2025)

Area	Images	Terms
stage 1-3		Stage 1-3  Non-Expressed
stage 4-5		Stage 4-5  Expressed   Zygotic    Zygotic starting stage 5   Localized    Subcellular localization patterns     Yolk plasm localization     Yolk cortex localization     Exclusionary patterns      Pole cell exclusion     Cytoplasmic foci
stage 6-7		Stage 6-7  Expressed   Zygotic    Yolk Nuclei     All yolk nuclei    Blastoderm nuclei     Subset blastoderm nuclei      Ventral blastoderm nuclei   Localized    Yolk plasm localization     Yolk plasm enrichment    Subcellular localization patterns     Nuclei-associated localization      Perinuclear localization       Perinuclear subset of nuclei        Perinuclear around yolk nuclei     Cytoplasmic foci
stage 8-9		Stage 8-9  Expressed   Zygotic    Yolk nuclei     Subset yolk nuclei    Blastoderm nuclei     Subset blastoderm nuclei      Expression in mesoderm      Expression in ectoderm      Expression in API   Localized    Subcellular localization patterns     Cytoplasmic foci     Basal localization     Apical localization    Embryonic localization patterns     Ventral localization     Anterior localization  Degraded   Degraded partially stage 8
stage 10-17		Stage 10-17  Expressed   Zygotic    Yolk nuclei    Muscles-embryo    Midgut     Midgut subset    Head   Localized

Area	Images	Terms

Area	Images	Terms

-performed via HCR-FISH using 3 probe sets targeting the RA isoform transcript

-seems to agree with modENCODE RNA-seq data

-interesting yolk and mesoderm patterns in trunk and head