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Number of genes | 10 | |
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It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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Posterior enriched |
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All over |
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Good probe on gel. |
Sub and peri nuclear with apical enrichment. Anterior cells only. Very specific and interesting. |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions' |
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Anterior midgut, stronger at the posterior midgut. |
Probe looks good on gel |
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Maternal RNA is posteriorly localized in yolk plasm and then in pole cells. |
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Good probe on gel In unfertilized eggs, and 2 nuclei embryos: I see osk very strongly expressed posteriorly (as expected) AND anterior expression as well (see 2 nuclei anterior 40x photo). The RNA probe shows anterior 'structures' (interesting and probably novel). |
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Again, perinuclear pole cell asymmetric expression. |
Pole cell asymmetric expression. I see small 'dots' around the pole cells, stronger in one 1/2 of the pole nuclei. |
Good probe on gel |
Posterior localization, even in unfertilized eggs. RNA accumulates asymmetrically around the pole buds with a 'fiber-like' texture. When nuclei divide, the RNA will stay in the nuclei that are closer to the posterior end. Very interesting! |