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Number of genes	4
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Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 6-7		Stage 6-7 Non-Expressed Degraded Degraded completely stage 6 Degradation maternal transcripts
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Specific tracheal branches Dorsal trunk Salivary glands Salivary glands lumen Ring gland Esophagus Ectoderm Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around tracheal nuclei Perinuclear around salivary gland nuclei Perinuclear around esophagus nuclei Perinuclear 'rings'

Area	Images	Terms

Area	Images	Terms

Good probe on gel, a bit low Low expression.

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Nuclei-associated localization Perinuclear localization Perinuclear subset of nuclei Perinuclear around yolk nuclei Embryonic localization patterns
stage 8-9		Stage 8-9 Non-Expressed
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Salivary glands lumen Proventriculus Posterior spiracles Specific posterior spiracle pattern PNS Pharynx Hindgut Germ cell nuclei Gonads Foregut Esophagus Ectoderm Polarized Ectoderm Blastoderm nuclei Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around pharynx nuclei Perinuclear around esophagus nuclei Perinuclear around ectoderm nuclei Cell junction associated

Area	Images	Terms

Area	Images	Terms

Good probe on gel.

Perinuclear esophagus localization, and others. Salivary glands, probably non-specific.

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Ubiquitous Medium expression (21-40ms exp) Maternal
stage 4-5		Stage 4-5 Expressed Weak expression (>41ms exp) Maternal Localized Subcellular localization patterns Exclusionary patterns Pole cell exclusion Basal exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 7 Degradation maternal transcripts
stage 8-9		Stage 8-9 Expressed Localized Subcellular localization patterns Cytoplasmic foci Degraded Degraded partially stage 8 Degradation maternal transcripts
stage 10-17		Stage 10-17 Expressed Zygotic Segmented pattern Salivary glands Proventriculus Posterior spiracles Pharynx Hindgut Specific hindgut area or cells Foregut Esophagus Ectoderm Anal pad Localized Subcellular localization patterns Perinuclear localization Perinuclear around hindgut nuclei Perinuclear around esophagus nuclei Cell junction associated

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Not expressed in fat body Borderline between 'not expressed' and 'low expression'
intestine		3rd Instar Larval Tissues Intestine Not expressed in the intestine
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues Nervous system Not expressed in nervous system
salivary gland		3rd Instar Larval Tissues Salivary gland Not expressed in salivary glands

Area	Images	Terms

Good probe.

Need better resolution images to determine exact sub-cellular localization.

Area	Images	Terms
stage 1-3		Stage 1-3 Expressed Weak expression (>41ms exp) Ubiquitous Maternal
stage 4-5		Stage 4-5 Expressed Maternal Localized Subcellular localization patterns Yolk plasm localization Yolk plasm enrichment Exclusionary patterns Pole cell exclusion Apical exclusion Embryonic localization patterns
stage 6-7		Stage 6-7 Expressed Ubiquitous Maternal
stage 8-9		Stage 8-9 Expressed Ubiquitous
stage 10-17		Stage 10-17 Expressed Zygotic Tracheal system Tracheal lumen Specific tracheal branches Polarized tracheal localization Segmented pattern Salivary glands Salivary glands - specific region Proventriculus Posterior spiracles Specific posterior spiracle pattern Pharynx Hindgut Specific hindgut area or cells Head Foregut Esophagus Ectoderm Polarized Ectoderm CNS Ventral nerve cord Clypeo-labral bud Blastoderm nuclei Localized Subcellular localization patterns Perinuclear localization Perinuclear polarized Perinuclear around tracheal nuclei Perinuclear around proventriculus nuclei Perinuclear around esophagus nuclei Nuclei-associated localization Nuclear in pharynx Nuclear in other cells Nuclear in ectoderm Accumulation nuclear subregion Accumulation entire nucleus Cell junction associated Apical enrichment Apical ectoderm

Area	Images	Terms
fat body		3rd Instar Larval Tissues Fat body Subcellular Localization - Fat body Cytoplasmic foci-fat body Expressed in fat body Fat body allover - Ubiquitous
intestine		3rd Instar Larval Tissues Intestine Expressed in midgut Midgut allover Expressed in malpighian tubules Subcellular localization in malpighian tubules Malpighian tubules - foci Malpighian tubules few foci Malpighian tubules allover
imaginal discs		3rd Instar Larval Tissues Imaginal discs Not expressed in imaginal discs
nervous system		3rd Instar Larval Tissues Nervous system Expressed in brain lobes Brain lobes allover Borderline between 'not expressed' and 'low expression'
salivary gland		3rd Instar Larval Tissues Salivary gland Expressed in salivary glands Salivary glands allover Borderline between 'not expressed' and 'low expression'
lymph glands and ring glands		3rd Instar Larval Tissues Lymph Glands and Ring Glands Ring gland Expressed in ring gland Ring gland allover Lymph Glands Subcellular localization in lymph glands Lymph Glands - Cytoplasmic foci Lymph Glands - Few cytoplasmic foci Expressed in lymph glands