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Number of genes | 26 | |
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Nuclear and sub-nuclear localization. |
Nuclear. |
Nuclear and sub-nuclear localization. |
Very nice pattern!! |
Very low probe on gel. Great in situ... No transcript associated with this probe, it may be a copia transposon element. |
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Good probe on gel. |
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Partly nascent, partly nuclear subregion. |
Partly nascent, partly nuclear subregion. |
Good probe on gel. |
Partly nascent, partly nuclear subregion. |
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Good probe on gel |
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Maybe a few small foci. |
Subnuclear localization. 2R - two dots, not one. |
Good probe on gel. |
Maybe a few foci. |
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Good probe on gel. |
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Partly nascent, partly nuclear subregion. |
Good probe on gel The transcript comes from chromosome 2L. It may be partly nascent, partly nuclear subregion. |
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Nuclear |
Good probe. |
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Very nice probe. |
Nuclear Pharynx, interesting expression and localization. |
The most anterior cell in the head are expressing. Interesting. |
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I see 'one dot' per nuclei in many cells... Partly nascent, partly nuclear subregion. |
Partly nascent, partly nuclear subregion. |
Good probe on gel. |
Partly nascent, partly nuclear subregion. |
Interesting patterns. |
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Male reproductive tissues |
Adult Tissues Male reproductive tissues Testis Expression level-TE Not expressed - TE Distal tip region-TE Not Expressed in distal tip region -TE Seminal Vesicle Expression level-SV Not Expressed - SV Ejaculatory Duct Expression level-ED Not Expressed - ED Accessory Gland Expression level-AG Not Expressed - AG |
Good probe on gel. No signal/not detected in adult male reproductive tissues. |
Good example to show sensitivity of the in situ protocol. |
Home-made probe. |
Salivary glands and tracheal lumen, probably non-specific. |
Good probe on gel. This insitu was done twice... similar reslutls obtained. |
Important note: The probe LP11739 overlaps with the 5' UTR of the 2mit gene (same location). |
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Home made probe to last exon. Covers all isoforms. |
Salivary glands and tracheal lumen, probably non-specific. |
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In many nuclei there appear to be several nuclear foci in addition to sites of nascent transcript synthesis. |
RNA seems to accumulate around most yolk nuclei and expressing blastoderm nuclei. |
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Good probe on gel No gene associated. |
Partly nascent, partly nuclear subregion |
Partly nascent, partly nuclear subregion |
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Nucelar Hemocytes |
Good probe on gel. |
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Home-made probe to exon 3. Good probe on gel. |
Interesting patterns! |
It is hard to distinguish if the axons in the larval ventral nerve cord are 'axons' or 'tracheal extensions |
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Male reproductive tissues |
Good probe. Very nice insitu patterns! |
Data from RE10437 probe. |
Intra-nuclear in balstoderm and yolk nuclei. |
Intranuclear localization. Amnioserosa specific pattern. etc. Salivary glands and tracheal lumen, probably non-specific. |
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Maybe some 'foci'. |
Nuclear - posterior spiracles Salivary glands, probably non-specific. |
Lowish probe on gel. Good in situ! |
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Salivary glands and tracheal lumen, probably non-specific. |
Good probe on gel. |
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Good probe on gel. Very low expression. |
Degraded a very low amount of transcript. |
Interesting! |
Nuclear |
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Allover with small foci. |
Sub-nuclear. |
Good probe on gel. Allover with small foci. |
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On chromosome 4... few cells with mild zygotic expression (anterior and dorsal). |
Good probe. |